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16 results • Page
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0
votes
11
replies
418
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How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq libraries?
STARSolo
scRNA-seq
STAR
snRNA-seq
MGI
1 hour ago by
atowns21
• 0
0
votes
5
replies
268
views
Is it possible to get a list of representative genomes from a past RefSeq release?
representative
ncbi
asembly
refseq
updated 12 hours ago by
GenoMax
141k • written 1 day ago by
Bertalan_Takacs
▴ 90
0
votes
4
replies
241
views
different FeatureCounts output for the same data
fpkm
Counts
Rsubread
rna-seq
17 hours ago by
sehriban.buyukkilic
▴ 10
1
vote
2
replies
190
views
alignment result
RNA-seq
samtools
hisat2
19 hours ago by
ahmad.sajad4541
• 0
0
votes
2
replies
143
views
Highest variable features in single cell data
single-cell
updated 5 hours ago by
Ram
43k • written 18 hours ago by
Kazo
• 0
0
votes
2
replies
177
views
Annovar using R package
Annovar
gnomAD
R
5 hours ago by
DKA
▴ 40
0
votes
1
reply
69
views
Normalize scRNAseq data to housekeeping genes to compare several datasets
RNA-sequencing
housekeeping
Single-cell
normalization
updated 5 hours ago by
ATpoint
82k • written 6 hours ago by
AaronJaime
• 0
0
votes
1
reply
138
views
PCA plot
DESeq2
PCAplot
updated 11 hours ago by
jkim
▴ 170 • written 22 hours ago by
Aaliya
▴ 10
0
votes
1
reply
380
views
GAPIT p-value significance threshold
GAPIT
p-value
GWAS
updated 10 hours ago by
ginellegrenier
• 0 • written 4 months ago by
Clayton
• 0
0
votes
0
replies
53
views
Is there a way to increase the automatic label text size in Cytoscape?
Cytoscape
8 hours ago by
avocado123
• 0
0
votes
0
replies
47
views
how to read graph_test output of monocle 3
monocle3
9 hours ago by
synat.keam
▴ 100
0
votes
0
replies
62
views
Why not use iBAQ for calculating differential abundance of proteins?
protein
maxquant
16 hours ago by
Aspire
▴ 300
0
votes
0
replies
37
views
Designing single-stable RNA molecules
structure
RNA
4 hours ago by
Edna
• 0
0
votes
0
replies
44
views
How to visualize/predict the final transcript from Delly output?
WGS
DELLY
4 hours ago by
simplitia
▴ 130
1
vote
0
replies
59
views
How to calculate reliable Ka/Ks or dN/dS ratio for genes of interest from VCF file
dnds
kaks
VCF
9 hours ago by
rohitsatyam102
▴ 850
0
votes
0
replies
44
views
How to calculate correlation coefficient for chipseq?
chipseq
bigwigsummary
correlation
8 hours ago by
Emily
▴ 10
16 results • Page
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Recent Votes
Answer: Seurat merge and batch correction
Comment: How to convert plink files to Hapmap Format
Comment: How to convert plink files to Hapmap Format
Comment: How to convert plink files to Hapmap Format
Comment: How to convert plink files to Hapmap Format
Comment: How to convert plink files to Hapmap Format
Answer: Missing protein (VEGF-A) in String db
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Recent Replies
Comment: Missing protein (VEGF-A) in String db
by
shalespringer
• 0
Thank you for replying here; this helped me figure out why MAPK10 was missing from my results. It was also marked as a pseudogene in the En…
Comment: How to convert plink files to Hapmap Format
by
Sofia
• 0
These are the first lines of the output: (Please is it normal to have NA in the P value column ?) CHR …
Comment: How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq librarie
by
atowns21
• 0
So I used the barcodes that I created (combos of positions 1-10 and 11-20) and I obtained similar alignment stats as the paper I pulled the…
Comment: How to convert plink files to Hapmap Format
by
Sofia
• 0
Thank you so much, it actually worked!
Answer: Rare Disease Variant Pathway Analysis
by
LauferVA
4.2k
Hi @efc1e545 , First a caveat. the information we most need in order to help guide you to a successful conclusion is not provided in thi…
Answer: Sequence read length shorter than flow cell specification
by
swbarnes2
14k
The company probably had you share your run with someone who needed the extra bases. So you get the extra bases free. Just use them unle…
Comment: how to combine multiple RNAseq count files into a single dataframe in R and unix
by
Ram
43k
Thank you, the `csvtk spread` is super useful. I usually import into R using `lapply` then `Reduce` using `merge` but this might be easier.
Comment: Annovar using R package
by
DKA
▴ 40
Thank you for your guidance. The thing is that I am unfamiliar with using such environments, unfortunately.
Comment: Treatment VS Control in Single Cell RNAseq analysis
by
ATpoint
82k
Open a new question, with details.
Comment: Normalize scRNAseq data to housekeeping genes to compare several datasets
by
ATpoint
82k
I recommend https://bioconductor.org/books/3.18/OSCA.basic/normalization.html as well as the "advanced" section in this book.
Comment: Why gatk VariantAnnotator required bam and coverage files
by
QX
• 0
thank you!
Comment: Treatment VS Control in Single Cell RNAseq analysis
by
kilcdincer
▴ 10
Hello, I have more or less same experimental setting and was wondering how you proceeded with your analysis? Can I reach its GitHub reposit…
Comment: BLAST using both nucleotides and taxonomic local databases
by
GenoMax
141k
> I ask this as some BLAST command fields as scinames or sblastnames do not give any output with a classic nt BLAST If that information is…
Answer: BLAST using both nucleotides and taxonomic local databases
by
5heikki
11k
This has been discussed [many times][1] [1]: https://www.biostars.org/p/76551/
Answer: why renaming Idents in Seurat object doesn't work?
by
Bioinfotec
▴ 10
I think when you give indent to seuObj : ```r Idents(seuObj) <- 'RNA_snn_res.0.1' ``` You may wrongly assign other value to it such as `…
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