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22 results • Page
1 of 1
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0
votes
10
replies
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Rockhoppper - Suspiciously low percentage of perfectly aligned reads
Rockhopper
RNA-seq
1 hour ago by
langziv
▴ 50
0
votes
9
replies
197
views
Deseq2 error
R
deseq2
2 hours ago by
sgadila
• 0
0
votes
4
replies
82
views
Understanding mother and father alleles in VCF file
snp
vcf
2 hours ago by
c.
• 0
0
votes
3
replies
285
views
QC of genetic data
PLINK
updated 6 hours ago by
bk11
★ 1.4k • written 4 days ago by
kl
▴ 10
0
votes
2
replies
166
views
How can I compute energy from a protein PDB file?
pdb
protein
updated 13 hours ago by
Matthias Zepper
4.1k • written 1 day ago by
4fzcgueyp5
• 0
0
votes
2
replies
269
views
Issues with featureCounts
featurecounts
rna-seq
differential-expression
updated 10 hours ago by
biofalconch
★ 1.0k • written 4 days ago by
Aime
• 0
0
votes
1
reply
107
views
Bam sort merge error?
bam
linux
sam
updated 16 hours ago by
Pierre Lindenbaum
157k • written 18 hours ago by
Athena
• 0
0
votes
1
reply
199
views
TEtranscripts Tool in Galaxy
RNAseq
Transposable_Elements
TE_Transcripts
updated 11 hours ago by
biofalconch
★ 1.0k • written 3 days ago by
gorizwango
▴ 30
0
votes
1
reply
63
views
WGCNA plotEigengeneNetworks error (coercion to logical)
WGCNA
plotEigengeneNetworks
RNA-seq
RStudio
updated 3 hours ago by
Ram
40k • written 5 hours ago by
Victor
• 0
0
votes
1
reply
449
views
Low percentage of 'Fraction Antibody Reads Usable' in Feature Barcode Cell Ranger output
scCITE-seq
feature-barcode
10x
scRNA-seq
updated 3 hours ago by
Ram
40k • written 5 months ago by
rocio.castellanos
• 0
0
votes
1
reply
220
views
Determine kit used for library preparation for whole exome sequencing from fastq files
Agilent
NGS
library-preparation
updated 3 hours ago by
Ram
40k • written 18 hours ago by
Harinder
• 0
0
votes
1
reply
83
views
Differentially Expressed Genes between two conditions (scRNA, single GEO dataset with multiple samples and no cell annotations)
scRNA-seq
RNA-Seq
Seurat
updated 2 hours ago by
bk11
★ 1.4k • written 3 hours ago by
prietto
• 0
0
votes
1
reply
28
views
Kimura% of calcDivergenceFromAlign output
repeatlandscape
repeatmasker
1 hour ago by
睿紘
• 0
0
votes
1
reply
417
views
FASTQ files for 30x 1KGP of Phase 3 data
phase3
fast
1kgp
45 minutes ago by
Qboy
• 0
0
votes
0
replies
67
views
Algorithmic Solutions for Resolving Overlapping Sequences in Sanger Sequencing of PCR Segments
Sanger
pcr
updated 8 hours ago by
Ram
40k • written 19 hours ago by
Amior
• 0
0
votes
0
replies
61
views
Detection of CNVs with copy number greater than 4 by PennCNV
copy-number
PennCNV
updated 8 hours ago by
Ram
40k • written 14 hours ago by
yamamoto.yasuyuki.n6
• 0
0
votes
0
replies
56
views
Adding means cluster from fviz_cluster on the PCA
fviz_cluster
updated 8 hours ago by
Ram
40k • written 10 hours ago by
ayaosama2111
▴ 10
0
votes
0
replies
44
views
Adding custom annotation to VEP output VCF
bcftools
VEP
vcf
4 hours ago by
avelarbio46
▴ 30
0
votes
0
replies
28
views
Help on dendrimer building
dendrimer
gromacs
molecular-dynamics
3 hours ago by
v.berriosfarias
▴ 130
0
votes
0
replies
57
views
DMRcate design
R
Epigenetics
EPIC
DMRcate
updated 8 hours ago by
Ram
40k • written 11 hours ago by
Bioinfonext
▴ 440
0
votes
0
replies
11
views
STAR Genome index Error
STAR
GenomeSAindex
44 minutes ago by
Prasanna
• 0
0
votes
0
replies
12
views
ATAC-Seq and RPKM
RPKM
ATAC-Seq
and
1 hour ago by
qudrat.nii
• 0
22 results • Page
1 of 1
Recent Votes
Answer: tensorQTL interaction issue
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Comment: How do you validate and verify your pipeline's software updates?
Answer: GSEA with GO and KEGG datasets
GSEA with GO and KEGG datasets
Answer: Exporting DEGs obtained from DESeq2
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hemantcnaik
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Answer: tensorQTL interaction issue
by
Solal
• 0
Writing a solution for anyone interested. The issue stems from a dosage error, some variant contains only heterozygous individuals and no h…
Answer: The Biostar Handbook. A bioinformatics e-book for beginners.
by
Qboy
• 0
Great book! I love using it to help my mentees to learn bioinformatics better. It would be also fantastic to compile all other necessary Pr…
Comment: Rockhoppper - Suspiciously low percentage of perfectly aligned reads
by
langziv
▴ 50
Thanks. I'll try.
Comment: How to get sample names and genotype for SNP in multi-sample VCF file
by
avelarbio46
▴ 30
Hi! I'm trying to parallelize your code (to run on a bunch of files), but it is not working because of multiple processes spawning. Do you …
Comment: Rockhoppper - Suspiciously low percentage of perfectly aligned reads
by
GenoMax
135k
> so I don't think the genome size is available. Perhaps not for your specific strain but plenty of other Kpn genomes are available and th…
Comment: Kimura% of calcDivergenceFromAlign output
by
睿紘
• 0
For additional information, "Coverage for each repeat class and divergence (Kimura)" is also the result from calcDivergenceFromAlign.pl
Comment: Rockhoppper - Suspiciously low percentage of perfectly aligned reads
by
langziv
▴ 50
By the way, I run Rockhopper on the untrimmed files, and the full match percentage grew to 20%-30%, and there are 1552 transcripts in the u…
Comment: Rockhoppper - Suspiciously low percentage of perfectly aligned reads
by
langziv
▴ 50
Here are the total bases in 4 fastq files (after adapter removal): 1,027,852,411, 1,041,895,083, 1,121,786,520, 1,299,721,929. I just rem…
Comment: Understanding mother and father alleles in VCF file
by
c.
• 0
actually they didn't tell me which relatives were from mother side and which from father side, you are right. But how can you find related …
Comment: Rockhoppper - Suspiciously low percentage of perfectly aligned reads
by
GenoMax
135k
Correct. Data that went into the assembly.
Comment: Rockhoppper - Suspiciously low percentage of perfectly aligned reads
by
langziv
▴ 50
Total bases (number of reads * read length) should be calculated after removal of adapters, right?
Comment: Getting BLAST to give output whilst running
by
Ken
• 0
If I understand correctly, Blast can tell you how many results it has gotten but it can't tell you how many it is going to get, therefore i…
Comment: Understanding mother and father alleles in VCF file
by
Ram
40k
I don't understand your logic. You don't know what phasing is but are confident it is not required, and your proof is that relatives were d…
Comment: Understanding mother and father alleles in VCF file
by
c.
• 0
It's from Nebula genomics. It must be possible to deduce parental alleles, since I converted the VCF file into a Myheritage VCF file and th…
Comment: Deseq2 error
by
sgadila
• 0
Sorry, I just edited my post.
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