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Recent Replies
Answer: Single Cell Rna Seq Using BD Rhapsody
by
Dr William Klubinski
▴
30
In general, the course of action should go as follows. Use FastQC to generate quality reports on your raw sequence data (Go to "NGS: QC and…
Comment: NarrowPeak format -Peak field
by
aaronlogsdon2001
•
0
Hi ATpoint - quick question. In my mind, 0-based means counting from 0. So if the peak value column shows 50, the summit is 52 (1 + 51), no…
Comment: Single Cell Rna Seq Using BD Rhapsody
by
dsull
★
4.2k
I think a good solution would be kallisto/bustools (or other solutions that work, like STARsolo or alevin-fry).
E.g. For kallisto/bustools…
Comment: DEGseq for multiple samples
by
Basti
★
1.5k
logFC and p-values are results of a differential analysis between the groups, it does not exist in the context of individual samples
Comment: Dot Plot using KEGG
by
smanzano250800
•
0
Thank you, I tried first the other answer (rpolicastro).
Answer: how to sort a fasta file
by
rpolicastro
12k
You can use seqkit.
```
seqkit sort -N in.fa
```
Alternatively if your sequences are indeed all just one line.
```
paste - - < in.fa | s…
Comment: how to run FacetsSuite wrapper scripts on command line
by
Dalal
•
0
Yes, the executable snp-pileup-wrapper.R is found in the path directory provided.
pwd
> /home/dalal/mut_analysis/variant-calling/face…
Comment: ggsave() bug in ggplot2?
by
barslmn
★
1.5k
Oh, I meant the shell command `file` which shows the resolution and dpi of the image file. ,:)
Comment: minimap's SAM file MAPQ value for the unique alignments
by
Mohd
•
0
Hi,
I am sequencing RNA of the GFP library, so I am mapping to GFP sequences (database having 5'UTR-CDS-barcodes_3'UTR of all the variants…
Comment: DEGseq for multiple samples
by
ALOUSH ALI
•
0
My question is about the output(differential gene expression analysis results) which contains results of logfoldchange and pvalues between …
Comment: ggsave() bug in ggplot2?
by
Medeea
•
0
>file("Figure1.jpg")
A connection with
description "Figure1.jpg"
class "file" …
Comment: DEGseq for multiple samples
by
Basti
★
1.5k
Your post is badly formatted, you should enhance the presentation. I do not understand your question because you created geneExpMatrix1 and…
Answer: Single Cell Rna Seq Using BD Rhapsody
by
GenoMax
129k
See answer here: https://www.biostars.org/p/9562237/#9562240
I don't think you can do this on Galaxy.
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