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Difference between RNA-sq and Transcriptome
Rat lung transcriptome
A: microarray, RNAseq, CEL, edgeR, etc. for DGE analysis
Converting SNP from chr:pos to rs number using PLINK?
How do I explain the difference between edgeR, LIMMA, DESeq etc. to experimental Biologist/non-bioinformatician
Answer: Cellranger sequencing
Answer: Is it possible to do DGE analysis using log 2 normalized data with EdgeR ?
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Maybe, I think the formal (1) is correct for most situations (for example the earlier version of GATK). However, there are some changes in …
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I don't think you can get that information specifically: https://kb.10xgenomics.com/hc/en-us/articles/360021902671-Can-we-detect-splice-va…
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Use `cut -d ' '`
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Hi, can you guide me how to compile the src folder? Many thanks!
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You can check [this][1] [1]: https://www.linkedomics.org/
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https://bioinformatics-core-shared-training.github.io/cruk-summer-school-2021/RNAseq/Markdowns/10_DE_analysis_with_DESeq2.Solutions.html …
Comment: Is it possible to do DGE analysis using log 2 normalized data with EdgeR ?
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Adaline.D
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I appreciate your help, LChart! I will use raw data in case of a substantial loss of power. (1) The publication did a PCA and then analy…
Answer: Is it possible to do DGE analysis using log 2 normalized data with EdgeR ?
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LChart
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Short answer: use LIMMA Long answer: edgeR uses a negative binomial model, and expects *counts* and not *values*. Please read section 2.8.…
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Wayne, both of these methods work beautifully. I really appreciate the detail you provided, thank you so much for your help!
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after alignment, it occurs in different sizes of gaps
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Not that I am aware of. Unlike methods such as salmon it does genome alignment and does not resolve the exact transcript expression composi…
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