Recent Locations •
All
France, just now
United Kingdom, just now
United Kingdom, just now
Israel, just now
Italy, just now
Serbia, 2 minutes ago
United Kingdom, 2 minutes ago
Recent Awards •
All
Popular Question to
Apex92
▴
200
Popular Question to
Gene_MMP8
▴
210
Popular Question to
Abhishek
•
0
Scholar to
Marco Pannone
▴
290
Recent Replies
Comment: Computing allele frequencies per individual for a set of SNPs in PLINK
by
pifferdavide
▴
100
Thanks and yes I had tried that but failed. I used the --const-fid (converts sample IDs to within-family IDs while setting all family IDs t…
Comment: Question regarding normalization in Seurat
by
ATpoint
62k
It is the sum of counts for that cell, so basically if a given cell has a given column in the count matrix it is the colSum of that column.
Comment: Clustered, annotated dotplot to scRNA-Seq data
by
fracarb8
▴
810
Have a look at `Clustered_DotPlot` from `scCustomize`.
Comment: Align short sequence against ONT reads
by
kirillkirilenko.tomsk
•
0
It won't work because minimap2 aligns against a large reference sequence. What I want to do is to align each read in .fastq files against s…
Comment: PCA plot for miRNA
by
struggler
•
0
Hi Abhishek,
For miRNA, first I predicted novel miRNA by miRDP2 and then aligning clean reads over it to get count file. 1>made database …
Answer: Align short sequence against ONT reads
by
colindaven
★
3.8k
Very weird question, but ....
- just use the gene/exon as a reference sequence
- use minimap2 to align the ONT reads against this
…
Comment: how to get a sequence from read fragments with ref sequence?
by
Aaron
•
0
Thank you. Let me try.
Comment: Determine sex/ gender of a mouse cell line via sequencing data
by
Amos Bairoch
▴
110
As I am trying to get as much information as possible on the sex of cell lines in the Cellosaurus, can you tell me which mouse cell line yo…
Comment: PCA plot for miRNA
by
Abhishek
•
0
What methods were used to quantify mRNA and miRNA from the raw fastq files ?
Comment: Sjdboverhang Option In Star
by
Thind amarinder
▴
260
This is what I found. https://biocorecrg.github.io/RNAseq_course_2019/alnpractical.html It usually equals to the minimum read size minus 1;…
Comment: Sjdboverhang Option In Star
by
Thind amarinder
▴
260
This is what I found. [https://biocorecrg.github.io/RNAseq_course_2019/alnpractical.html][1] It usually equals to the minimum read size min…
Comment: fineSTRUCTURE Resources
by
Juan MONROY-NIETO
•
0
try this https://www.biostars.org/p/221872/#391811
Comment: Error with TCGA package - TCGAbiolinks
by
Kevin Blighe
81k
Please post on Bioconductor Support: https://support.bioconductor.org/
Comment: Ribosomal Depletion
by
andres.firrincieli
★
2.5k
It is easier and do his job. But if you are planning to do differential expression then follow @grant.hovhannisyan suggestion
Comment: Ribosomal Depletion
by
grant.hovhannisyan
★
2.4k
then I recommend not bothering about removing the rRNA data during the mapping - just map all your data to the entire reference, do the cou…
Traffic: 1866 users visited in the last hour
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by the
version 2.3.6
version 2.3.6