myvariant Posts

C: Adaptor trimming for RNAseq by genomax ♦ 58k
What does the summary stats say about the trimming? Perhaps there are no adapters (always theoret...
C: Adaptor trimming for RNAseq by genomax ♦ 58k
Sure. As long as the leading sequence is identical for all adapters.
C: Why is 0/0 homozygous reference call in VCF useful information? by lillian.thistlethwaite • 0
Ah, so I think what both Pierre and Karl are saying is "0/0" help to rule OUT a disease if you kn...
C: Adaptor trimming for RNAseq by MAPK • 1.3k
No, they did not provide anymore information, except for that adaptor sequence. The fastq header ...
C: differently expressed genes by grant.hovhannisyan • 1.2k
You can check by qPCR.
C: Deletion and Duplication mutations alignment by jrj.healey ♦ 8.4k
I got a different alignment from clustal which broke it at a different place, one of the mismatch...
C: Adaptor trimming for RNAseq by MAPK • 1.3k
Thanks for your answer. So just to be clear, should I just have >adaptor1 AGATCGGAAG...
A: Run GSEA with custom gene sets? by h.mon ♦ 21k
For the command-line, you have to prepare the gene-set in [gmt format][1], and pass it with `-g g...
A: Adaptor trimming for RNAseq by genomax ♦ 58k
I would not worry about what `NNNNN` is, if the core sequence you are trying to remove is common....
C: Adaptor trimming for RNAseq by lieven.sterck • 3.1k
and they did not provide any additional information, eg. what the index might be then? long sho...
C: Deletion and Duplication mutations alignment by RamRS ♦ 19k
Please use the formatting bar (especially the `code` option) to present your post better. I've do...
C: Adaptor trimming for RNAseq by MAPK • 1.3k
Yes, it was provided by the sequencing facility.

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