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questions
1
vote
3
replies
737
views
How to check gene properties (eg GC, repetitiveness, average expression level)?
deseq2
kallisto
RNA-seq
updated 15 months ago by
Ram
43k • written 17 months ago by
bioinfo
▴ 150
0
votes
10
replies
1.3k
views
Can I demultiplex run based on i5 sequence?
demultiplexing
bcl2fastq
updated 17 months ago by
GenoMax
142k • written 17 months ago by
bioinfo
▴ 150
3
votes
13
replies
3.2k
views
How to choose parameters for kallisto single end mode?
kallisto
RNA-seq
updated 11 months ago by
Istvan Albert
100k • written 18 months ago by
bioinfo
▴ 150
7
votes
3
replies
2.0k
views
Should I merge my technical replicates for bulk RNA seq?
RNA-seq
DESeq2
updated 15 months ago by
Ram
43k • written 18 months ago by
bioinfo
▴ 150
4
votes
4
replies
1.0k
views
Can I perform DE analysis with Seurat if I only have one sample per condition?
single
RNA
Seq
cell
seurat
updated 18 months ago by
LChart
3.9k • written 18 months ago by
bioinfo
▴ 150
0
votes
0
replies
364
views
How do I get estimated gene counts for a reference that contains GFP using Kallisto?
gene
ensembl
tximport
kallisto
19 months ago by
bioinfo
▴ 150
4
votes
4
replies
1.3k
views
How can I confirm that I installed bcl2fastq correctly?
bcl2fastq
linux
ubuntu
20 months ago by
bioinfo
▴ 150
3
votes
2
replies
619
views
Why do I get a positive gene expression with qPCR for one my genes but in with when doing bulk RNA seq I get a count of 0?
gene
qpcr
kallisto
seq
expression
rna
updated 21 months ago by
dsull
★ 6.0k • written 21 months ago by
bioinfo
▴ 150
1
vote
3
replies
788
views
How to filter .fa file by gene_biotype
rna-seq
kallisto
updated 23 months ago by
Ram
43k • written 23 months ago by
bioinfo
▴ 150
0
votes
7
replies
1.2k
views
6 follow
How to create kallisto index that does not include ribosomal genes
enseml
kallisto
RNA-seq
updated 17 months ago by
Ram
43k • written 23 months ago by
bioinfo
▴ 150
0
votes
0
replies
372
views
Replicates in sc-RNA seq have different amount of cells-Can I still use them for the analysis?
Cell
seurat
Single
updated 23 months ago by
ATpoint
82k • written 23 months ago by
bioinfo
▴ 150
2
votes
4
replies
1.0k
views
Can I use the transcripts_to_genes.txt file from kallisto for bulk RNA seq data?
kallisto
updated 2.0 years ago by
Ram
43k • written 2.1 years ago by
bioinfo
▴ 150
2
votes
3
replies
1.1k
views
Ensembl version for mapping and annotating genes
rna-seq
kallisto
updated 23 months ago by
Ram
43k • written 2.1 years ago by
bioinfo
▴ 150
6
votes
2
replies
593
views
Ensembl version
ensembl
kallisto
updated 16 months ago by
Ram
43k • written 2.1 years ago by
bioinfo
▴ 150
2
votes
2
replies
2.4k
views
Cellranger command for RNA velocity
cellranger
Cell
velocity
RNA
Single
updated 2.3 years ago by
Friederike
8.9k • written 2.3 years ago by
bioinfo
▴ 150
4
votes
2
replies
935
views
Estimated gene counts with tximport
RNA-seq
ensembl
tximport
kallisto
updated 23 months ago by
Ram
43k • written 2.3 years ago by
bioinfo
▴ 150
3
votes
3
replies
1.3k
views
Will Kallisto show a decrease in the TPM value if I have knocked out 2 of the 8 exons from a gene?
RNA-seq
updated 23 months ago by
Ram
43k • written 2.4 years ago by
bioinfo
▴ 150
2
votes
2
replies
1.1k
views
How to demultiplex a single indexed library on a dual indexed flow cell?
Cell
Single
updated 2.5 years ago by
GenoMax
142k • written 2.5 years ago by
bioinfo
▴ 150
68 results • Page
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