I have a "complete" genome of a plant in a fasta file (16Go) I need to find some part of it, so I use blast. My first issue, when creating a database, it only accept 1Go… meaning most of my reads are not considered. How to proceed to get all my reads in a given database. Then, If I blast a sequence from another species in my db, how to get the output in a fasta file? By the way, is it possible to use a local blast through a browser, looking like NCBI, instead of a terminal? Eventually, is it possible to obtain a consensus sequence from those sequences? Or doing a local assembly.
Thanks in advance