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Take your STAR alignment and sum all the counts for each gene. You can either do this by providing an annotation to STAR, or by running fea…
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jesus its in the Genbank file and I was looking at the refseq! I m such a moron - thanks for pointing it out -sorry about that :(
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I wonder how the `Vlnplots` will look if you normalize the data using `NormalizeData()` function in `Seurat`. The flat line in your plot ar…
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> Does that mean that the reads that are not mapped to my trascriptome are not exons/coding genes?
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For doing this, should I just look at the log.out.file?
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The command lines I wrote out for you are the ones I actually used. My original reference to bedtools intersect was because I assumed the b…
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I just thought it was weird that considering each dot is a cell their expressions were all on a single line instead of being spread out lik…
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![enter image description here][1] I am already using Seurat V5 I think. I am using SCTransform as it was recommended by the seurat page. I…
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