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Forum: Site Use Guidelines
meta biostars Guidelines
updated 20 days ago by 43k • written 14.6 years ago by 100k
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Forum: Bioinformatician who was retaliated against after reporting discriminatory harassment - Please help me navigate getting out of the situation I'm in
Career advice
updated 28 days ago by ★ 2.5k • written 29 days ago by • 0
6
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Forum: Ideal PC configurations and operating system for bioinformatics laboratory
PC
11 days ago by Estevão ▴ 10
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Forum: VCF bulk handling
bcftools SnpEff WGS VEP VCF
26 days ago by Javier • 0
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Forum: Bioinformatics: Where do I start
Sequencing
updated 27 days ago by 43k • written 27 days ago by ▴ 20
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Forum: Improvement on automatic annotation of single-cell RNA-seq data from the literature using LLM
annotation scRNA llm
20 days ago by yxwucq • 0
6 results • Page 1 of 1
Recent Votes
Comment: How does gene length effect the number of reads mapped
Comment: How does gene length effect the number of reads mapped
Comment: How does gene length effect the number of reads mapped
Comment: How does gene length effect the number of reads mapped
A: Filtering of VCF, INFO DP or FORMAT DP
What is the difference between GRCh37 and hs37? And hg19?
Answer: Duplicated sequence samtools
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Recent Replies
Comment: Help with IGV abbreviation by GeneC • 0
Thank you for the link and details
Comment: Help with IGV abbreviation by GeneC • 0
Thank you very much for helping with the details.
Comment: How does gene length effect the number of reads mapped by Chen • 0
thank you :)
Answer: Filtering based on alternate allelic balance by Arton • 0
I found the answer when using bcftools. Is there is a way to do this with FilterVcf? bcftools filter --include '(FMT/AD[0:1])/(FMT/A…
Comment: Help with IGV abbreviation by GenoMax 142k
Those are SAM format fields. Check section 1.4 here: https://samtools.github.io/hts-specs/SAMv1.pdf > 1:2114:12111:13792 That is part of …
Answer: Help with IGV abbreviation by Mathew ▴ 120
Hi, here is a breakdown of each part that you asked about: **flag 99**: This indicates various properties of the read alignment. In this c…
Comment: Kraken2 database by Mathew ▴ 120
I don't see any databases with just pathogenic bacteria genomes from just a quick search, I would imagine that using the Standard-16 databa…
Comment: Galaxy StringTie error by Mathew ▴ 120
I am not sure if this is true for Galaxy, but when I code in Python, SyntaxWarning: invalid escape sequence '' occurs when you want to add …
Comment: Can diamond prepdb be used to make a taxonomically aware database? by GenoMax 142k
Should be possible: https://www.biostars.org/p/430366/ Are you using an older version of `diamond`? Latest versions of `diamond` can use N…
Comment: SNPEff database building error by Fungal genetics • 0
Hi, Below SNPEff database building error I am getting. "FATAL ERROR: Most Exons do not have sequences!" The headers are correct and sam…
Comment: Extracting named fasta sequences according to list with Biopython by Rubayetul • 0
the last line of SeqIO.write(record.....'fasta') in a for loop will input the the last record into new fasta file and it will only contain …
Comment: How to process Bulk WES data? by GenoMax 142k
You could use `sarek` (nf-core's exome pipeline) if you don't want to customize or reinvent : https://nf-co.re/sarek/3.4.2
Comment: Empty .best and .sing2 Files After Running Demuxlet by Ram 43k
Please use the formatting bar (especially the `code` option) to present your post better. You can use backticks for inline code (\`text\` b…
Answer: FINAL CALL: 8th Berlin Summer School in NGS Data Analysis - Only a few last plac by David Langenberger 11k
Two last seats have just become available. Apply now, if you want them.
Comment: featureCounts output summary assigned value higher than uniquely mapped reads fr by GenoMax 142k
You always need to add the following option when you are using `-p` to count paired-end reads. --countReadPairs If specified, fragm…
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