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What percent of reads should align to a reference gene to successfully validate it through RT-PCR?
gene rt-pcr rna-seq validation written 2 hours ago by Glory Basumata • 10 • updated 57 minutes ago by lshepard • 210
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Identify jumbled transcripts (scrambled exons) using HISAT2, stringtie, and GFFcompare?
stringtie rna-seq gffcompare hisat2 written 2 hours ago by wolfgang.rumpf • 0
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How to use limma for a categorial outcome variable? Where outcome is the cohort status and predictor is gene expression.
next-gen rna limma rna-seq written 21 days ago by halo22 • 120 • updated 2 hours ago by Biostar ♦♦ 20
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how to interpret hisat2 result
assembly alignment rna-seq written 10 hours ago by fatimarasool135 • 20
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Request for EnsDb (.Hsapiens.v95) download link
rna-seq written 7 hours ago by ShahiRB • 0 • updated 7 hours ago by jared.andrews07 • 2.2k
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LogFC calculation in multiple comparisons
rna-seq edger deseq written 1 day ago by elb • 160 • updated 23 hours ago by RamRS ♦ 21k
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How to normalize NCI-60 gene expression data
normalization nci rcellminer rna-seq R written 23 hours ago by hAjmal • 30
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90
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Error in RNA editing tool sprint
software error alignment rna-seq written 25 days ago by hirahameed222 • 0 • updated 1 day ago by cchmczhang • 0
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How can I use bedtools complement, sam mapping file, and my gtf file to get number of reads for intergenic regions?
rna-seq written 1 day ago by O.rka • 90
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5 follow
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Fold change cut off enrichment analysis
enrichment cutoff ontology rna-seq fold change written 1 day ago by Pietro • 50 • updated 1 day ago by shawn.w.foley • 400
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Job: Bioinformatician / Neuroscience / Heidelberg
neuroscience rna-seq data integration job written 22 days ago by slezak • 0
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What is the best way to rank genes for GSEA?
gene ranking gsea go rna-seq written 1 day ago by Gabriel • 10 • updated 1 day ago by Pietro • 50
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Tool: Arriba: Fast and accurate gene fusion detection from RNA-Seq data
variant calling tool cancer fusion rna-seq gene written 14 months ago by uhrigs • 70
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16S rRNA extraction of assembled genome bins
genome gene rna-seq written 9 weeks ago by luyang1005 • 20 • updated 1 day ago by Asaf ♦ 5.5k
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RNA Seq analysis of rice samples
next-gen rna-seq sequencing written 1 day ago by alim.hcu • 0
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Differential analysis show different results with edgeR and in box plot with t-test
differential analysis R boxplot rna-seq edger written 1 day ago by newbie • 20
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Cufflinks takes too long time on my PC
next-gen rna-seq written 1 day ago by scheme4193 • 0
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6 follow
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Kallisto-Sleuth or Kallisto-Deseq2?
rna-seq written 17 months ago by Mozart • 100
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How to evaluate the similarity between two different samples by using RNA-Seq?
gene rna-seq written 3 days ago by dz2353 • 70 • updated 1 day ago by Charles Warden ♦ 6.5k
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5 follow
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Infer experiment - Failed to determine strandin paired end stranded RNA-seq
hisat2 paired end star rna-seq stranded written 4 months ago by GiusiG • 0 • updated 1 day ago by zoegward • 60
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Biologic replicates with human samples
rna-seq written 2 days ago by AS • 0 • updated 1 day ago by jared.andrews07 • 2.2k
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HISAT2 question (hisat2_extract_splice_sites )
rna-seq bash written 2 days ago by zhu.ge • 0
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On the practical use of estimateDisp function
rna-seq edger written 2 days ago by elb • 160 • updated 2 days ago by RamRS ♦ 21k
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Bioinformatics grant application links
next-gen rna-seq written 3 days ago by B- for bioinformatics • 20 • updated 2 days ago by Jeremy Leipzig ♦ 18k
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GSEA for glioblastoma multiforme
rna-seq written 17 months ago by nelusia17 • 0 • updated 2 days ago by gabriel.rosser • 130
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5 follow
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Bcl2fastq conversion according to samples (demultiplexing)
next-gen bcl2fastq rna-seq demultiplexing written 2 days ago by 1234anjalianjali1234 • 30
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6 follow
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RNAseq: What's the accurate way to test whether a set of N genes is differentially expressed?
rna-seq written 2 days ago by francois • 10 • updated 2 days ago by Devon Ryan ♦ 89k
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162
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Gene and pseudogenes
rna-seq written 2 days ago by sabaghianamir70 • 0
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How to filter sequencing reads in a fastq file by size?
ribo-seq fastq size rna-seq filter written 4 days ago by Beginner • 0 • updated 2 days ago by emeline.a.favreau • 10
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Job: Bioinformatician - Genome Biologics - Accelerating Drug Development
next-gen python job machine learning R rna-seq written 2 days ago by Rahul Sharma • 560
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