Biostar Forum

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personal-genomics 23andMe

updated 26 days ago by Ram 38k • written 9.3 years ago by Istvan Albert 97k

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20k

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37 follow

Workshop Training Courses

updated 28 days ago by Lars ▴ 970 • written 7.9 years ago by Deepak Tanwar ★ 4.2k

105

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11k

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13 follow

epigenetics

updated 27 days ago by Ram 38k • written 9.6 years ago by ugly.betty77 ★ 1.1k

126

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27k

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16 follow

NGS adapter RNA-Seq

updated 18 days ago by Ram 38k • written 6.5 years ago by Lars ▴ 970

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14k

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8 follow

NGS alignment genome RNA-Seq sequence

updated 18 days ago by Ram 38k • written 4.2 years ago by David Langenberger 9.9k

137

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20k

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Popular Question to Alex S ▴ 20

Scholar to i.sudbery 16k

Teacher to AtenaLia ▴ 30

Recent Replies

Comment: split fasta file to train deep learning model by shenwei356 7.9k

Excluding sequences containing any letter not belonging to the 20 [amino acids letters](https://github.com/shenwei356/bio/blob/master/seq/a…

Comment: split fasta file to train deep learning model by pinheirofabiano ▴ 10

@shenwei356, thank you very much for your help, perfect! But now I realized that some fasta sequences contain the letter "B", which is ou…

Comment: Most efficient way to run Diamond against a very very large database (i.e., NCBI by Mensur Dlakic ★ 23k

I think it depends on the speed of your local disks and the memory amount. On a single node, breaking up the database doesn't sound like a …

Comment: Sample size for population genetics by Jeremy Leipzig 21k

Other populations just make the model harder to generalize, not easier.

Comment: Are We Rude/Do We Expect Too Much From People Asking Questions On This Forum? by Vincent Laufer ★ 2.5k

i think there is some of that - my most upvoted comment of all time was a dismissive comment i made while extremely tired. for context i ha…

Comment: Molecular biologist / clinical pharmacologist (f/m/d) by Jeremy ▴ 770

Is this job on site in Vienna or remote?

Comment: smoothing or binning bigWig file by rls_08 ▴ 40

if you use bigwigCompare , that would not create a sliding window, but instead, it will output the mean for each bin, according to the -bin…

Comment: Sample size for population genetics by zimmer.schweiz • 0

Thank you so much for your answer. For common polygenic diseases, would it make sense to increase the number of populations sampled, in ord…

Answer: Sample size for population genetics by Jeremy Leipzig 21k

For a rare penetrant monogenic disease? A few cases. For a common polygenic disease or trait? 500k-10M

Answer: using GRanges metadata to constrain overlap searches between objects by seidel 11k

Rather than do all overlaps all the time for all samples, why not restrict the data by sample when you can. The code below works about 4 ti…

Comment: How to get gene from PSIBLAST resuts by Tom • 0

Hello, thank you for your answer, I have a question, Why the information from the GFF file and from the feature table is different? I see …

Comment: SNP ID (rsID) to Chr no. and Position by Jewahir • 0

Yeah, thank you for that!!

Comment: 1000 genomes hg38 with dbSNP rsid by Ram 38k

It should be pretty straightforward. Just to save you some pain, run these on the 1000g VCF once you download it: 1. `vt decompose -s` to …

Comment: 1000 genomes hg38 with dbSNP rsid by Vince ▴ 150

Yeah, I had some hope that I wouldn't need to mess with doing this ...

Answer: counting the unmapped reads by chemkhi.ali13 • 0

> Hi all, > > I have a sam file, my supervisor asked me to count the number of > unmapped reads, which command I should use? > > sam…

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