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12 results • Page
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variant allelic fraction
filter
vep
analysis
output
columns
updated 14 hours ago by
Pierre Lindenbaum
154k • written 16 hours ago by
malm
• 0
0
votes
3
replies
288
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(ERR): "index/Trinity.fa" does not exist or is not a Bowtie 2 index Exiting now ...
bowtie2
align_stats
trinity
19 hours ago by
eimanpharmacist
▴ 20
1
vote
2
replies
158
views
Getting species names and taxa id from assembly accession number
efetch
biopython
entrez
updated 1 hour ago by
GenoMax
129k • written 21 hours ago by
kwhiggins27
▴ 10
0
votes
2
replies
67
views
CNNScoreVariants Error
CNNscore
1 hour ago by
bestone
▴ 10
1
vote
2
replies
197
views
Combination of ROC CURVE
roccurve
R
AUC
14 hours ago by
Maria17
▴ 20
0
votes
1
reply
144
views
Where to get the molecular subtype information of STAD sample in TCGA
TCGA
database
updated 20 hours ago by
Hamid Ghaedi
2.9k • written 1 day ago by
younglin113
▴ 50
0
votes
1
reply
121
views
Pairwise Alignment
Pairwise
Alignment
updated 16 hours ago by
WouterDeCoster
47k • written 17 hours ago by
Fatemeh
• 0
0
votes
1
reply
15
views
Difference between USCS exon coordinates and ensembl
exons
Ensembl
updated 3 minutes ago by
ATpoint
72k • written 46 minutes ago by
amy__
▴ 50
0
votes
0
replies
27
views
Prediction of altrered metabolites from altered gene expression
Gene
Metabolites
3 hours ago by
english.server
▴ 290
0
votes
0
replies
83
views
Is it possible to add a dendrogram as a top annotation using ComplexHeatmap ?
Dendrogram
R
Heatmap
ComplexHeatmap
19 hours ago by
Manuel
• 0
0
votes
0
replies
67
views
Perl. How to retrieve data until a keyword in GenBank?
fasta
sequence
GenBank
perl
1 hour ago by
Fungi-Beware!
▴ 10
0
votes
0
replies
20
views
Why sone miRNAs are not present in TCGA data
TCGA
miRNA
RNA-seq
2 hours ago by
nicolo.gualandi
• 0
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SeqKit: a cross-platform and ultrafast toolkit for FASTA/Q file manipulation in Golang
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Recent Replies
Comment: How to calculate TPM from featureCounts output
by
rpolicastro
12k
If you want to go that route, yes.
Comment: Difference between USCS exon coordinates and ensembl
by
ATpoint
72k
What did you download from UCSC? 1-off usually means one file is zero-based and the other 1-based. BED files from UCSC are 0-based, Ensembl…
Comment: Alignment of case vs. control from different origin
by
sativus
• 0
Thank you kindly for the explanation, it is highly appreciated. I suppose i was a bit confused as you quite often see people validating the…
Answer: Gene enrichment analysis
by
nux
▴ 10
Hello. For this you have to select 'hyper' as your method and then provide a complete list of genes which both include the candidate and no…
Comment: CNNScoreVariants Error
by
bestone
▴ 10
Thank you for replying I updated it worked but it gives another Error A USER ERROR has occurred: Invalid argument ' -V'.
Comment: Impute haplotypes (ImputePipelinePlugin) execution error - PHG
by
jrodrigu
• 0
Thank you so much. I made the suggested changes. However, I still have the same problem. Please check the new log file. https://github.c…
Comment: Getting same value for start and end position, "DNA methylation"
by
ATpoint
72k
That's a comment, not an answer, please use `ADD COMMENT`.
Comment: Getting same value for start and end position, "DNA methylation"
by
bioinfo_ga
▴ 50
Are you working on bisulphite kind of data or chipseq ????
Answer: How to calculate TPM from featureCounts output
by
bioinfo_ga
▴ 50
hi , You can use a python package rnanorm [https://pypi.org/project/rnanorm/]. The input required are your read count values from feature …
Comment: How to split a scRNA reads BAM or FASTQ file to a separate file for each cell by
by
biofalconch
▴ 580
Hey, if you arelady have the barcodes, you could use samtools `samtools view -h -b -f CB:Z:TAAGAGATCCTATGTT > TAAGAGATCCTATGTT.bam` Hope…
Answer: How to split a scRNA reads BAM or FASTQ file to a separate file for each cell by
by
biofalconch
▴ 580
Here is a code that should work, but just like everyone else in the comments I'm a little confused why would you need to separate them: …
Comment: Make a BedGraph file
by
kirillkirilenko
▴ 10
It works, thank you!
Comment: CNNScoreVariants Error
by
Pierre Lindenbaum
154k
check you have a complete environment for GATK , including the python module "keras". https://gatk.broadinstitute.org/hc/en-us/articles/360…
Comment: How to split a scRNA reads BAM or FASTQ file to a separate file for each cell by
by
Cathal
• 0
Did you have any luck in splitting the BAM file based on the 10x cell barcode? I would like to split a BAM file based to only include 5 spe…
Answer: Getting same value for start and end position, "DNA methylation"
by
ATpoint
72k
genomation is based on GenomicRanges which uses 1-based coordinates. https://www.biostars.org/p/84686/ Since a single CpG has a length o…
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