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Recent Replies
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Hello,
What approach could you recommend for a large genome (3Gb) with about 10k scaffolds?
I'm having problems with RAM when processing t…
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Very good question and analysis. I personally only tried SoupX so can't weigh in on the comparison. This might be not really the issue here…
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Try following the same logic as the guide which adds the labels about halfway through each pie slice value. Check column's name in script b…
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sorry for that, this is first time i use it.
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thank sir, i will check it again
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Ok, I will response to you here as soon as possible.
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How much memory do you have available? For `nt` database you are going to need 30-40G minimum. You may simply be running out of memory.
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Noted! Thanks very much for your help!
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i'm using linux. I was use 3 file.fasta to debug and it is nucleotide file, and my blast -h still work
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Thank you! I ended up using the transcripts_to_genes instead of biomart.
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Please be specific. Which TCGA subset, what did you try? Show code.
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A good tool for mitochondrial genome assembly is GetOrganelle (https://github.com/Kinggerm/GetOrganelle)
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Please do not use `bioinformatics` as a tag unless your post in about the field of bioinformatics itself.
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