19 days ago by
David Langenberger
11k
12
votes
2
replies
455
views
8
votes
2
replies
786
views
3
votes
0
replies
188
views
updated 2 days ago by
Ram
43k
•
written 3 days ago by
Claire Watson
▴
60
3
votes
0
replies
125
views
updated 6 days ago by
Ram
43k
•
written 6 days ago by
carlopecoraro2
★
2.5k
2
votes
0
replies
121
views
updated 20 days ago by
Ram
43k
•
written 20 days ago by
carlopecoraro2
★
2.5k
2
votes
0
replies
125
views
2
votes
0
replies
127
views
updated 18 days ago by
Ram
43k
•
written 18 days ago by
Transmitting Science communication
▴
70
2
votes
0
replies
116
views
19 days ago by
Transmitting Science communication
▴
70
1
vote
0
replies
132
views
1
vote
0
replies
177
views
updated 21 days ago by
Ram
43k
•
written 23 days ago by
carlopecoraro2
★
2.5k
0
votes
0
replies
134
views
updated 8 days ago by
Ram
43k
•
written 8 days ago by
carlopecoraro2
★
2.5k
0
votes
0
replies
194
views
updated 11 days ago by
Ram
43k
•
written 11 days ago by
carlopecoraro2
★
2.5k
0
votes
0
replies
178
views
0
votes
0
replies
188
views
updated 17 days ago by
Ram
43k
•
written 18 days ago by
carlopecoraro2
★
2.5k
0
votes
0
replies
148
views
updated 26 days ago by
Ram
43k
•
written 26 days ago by
carlopecoraro2
★
2.5k
0
votes
0
replies
183
views
15 days ago by
carlopecoraro2
★
2.5k
0
votes
0
replies
81
views
11 hours ago by
carlopecoraro2
★
2.5k
0
votes
0
replies
122
views
updated 21 days ago by
Ram
43k
•
written 21 days ago by
carlopecoraro2
★
2.5k
0
votes
0
replies
159
views
updated 3 days ago by
Ram
43k
•
written 4 days ago by
carlopecoraro2
★
2.5k
0
votes
0
replies
119
views
updated 3 days ago by
Ram
43k
•
written 5 days ago by
David Langenberger
11k
0
votes
0
replies
145
views
0
votes
0
replies
87
views
7 days ago by
carlopecoraro2
★
2.5k
0
votes
0
replies
178
views
updated 11 days ago by
Ram
43k
•
written 11 days ago by
carlopecoraro2
★
2.5k
0
votes
0
replies
117
views
updated 5 days ago by
Ram
43k
•
written 5 days ago by
carlopecoraro2
★
2.5k
0
votes
0
replies
149
views
28 days ago by
carlopecoraro2
★
2.5k
Recent Votes
Recent Locations •
All
United States, 1 minute ago
Bologna, 1 minute ago
United States, 2 minutes ago
United States, 3 minutes ago
United States, 3 minutes ago
United States, 5 minutes ago
United States, 5 minutes ago
Recent Awards •
All
Popular Question to
cwwong13
▴
40
Popular Question to
v.berriosfarias
▴
140
Popular Question to
scarey
•
0
Scholar to
e.r.zakiev
▴
200
Popular Question to
Bine
▴
60
Popular Question to
Deepak Tanwar
★
4.2k
Popular Question to
Chris
▴
260
Recent Replies
Comment: Batch effects : ComBat or removebatcheffects (limma package) ?
by
cwwong13
▴
40
@kevin May I know whether your comments and edits were mainly based on the fact that "Previously, [crazy] people had been using the origina…
Comment: Programmatically retrieving positions of protein active site residues
by
Wayne
★
2.0k
Wow, I was shocked at this AI answer that looks to be a good start for using UniProt. Sharing it in case it is useful. (It will work in ses…
Comment: NGS forensics: how to know if data is fabricated
by
noodle
▴
570
> Can you clarify what you mean by "100% of reads pass cutadapt, even
> though 70% of reads contain adapters and get trimmed.
The header o…
Comment: Software to separate reads from different individuals
by
GenoMax
141k
If you have independent information available (e.g. genotype data for both individuals) then you *may* be able to assign reads based on the…
Answer: Extract protein sequence
by
Matthias Zepper
4.5k
You didn't mention what kind of organism you are working with and what kind of protein you are looking for?
If it happens to be a microbia…
Comment: How to use limma to find differentially expressed genes in response to a continu
by
pairedttest
▴
10
Thank you Gordon for your expertise. One question that I have now that I have a list of DE genes is how to interpret logFC of this continuo…
Comment: FastQC Quality per tile and per sequence behaving strange after using Cutadapt
by
salias
•
0
Yes I plan to use QIIME2, but I'm Cutadapting outside QIIME so its easier to MultiQC without importing/unzipping QZAs (and QIIME2 importing…
Comment: FastQC Quality per tile and per sequence behaving strange after using Cutadapt
by
GenoMax
141k
> 3' adapters were removed aprox. 90 times each one, so there sohuld not be read-through at all here
Then you have data that has poor Q sc…
Comment: FastQC Quality per tile and per sequence behaving strange after using Cutadapt
by
salias
•
0
Following your advice, I checked the primer and adapters sequences and edited the question accordingly
Comment: How should I make kallisto indexes?
by
dsull
★
5.9k
We can't phase them out per se, because ``kallisto index`` is still the engine responsible for creating the index under the hood (and there…
Comment: When to use .vcf or .gvcf files from GATK HaplotypeCaller?
by
Medhat
9.7k
"./." in your gVCF files typically indicates a lack of information or uncertainty about the genotype at that particular position.
This co…
Comment: FastQC Quality per tile and per sequence behaving strange after using Cutadapt
by
salias
•
0
Fungal ITS2 region (length is really variable so read-through can be an issue)
Answer: Is it possible to bulk download files from GEO repository?
by
noodle
▴
570
Also try the NCBI AWS bucket transfer tool. It's extremely convenient, however it's not instantaneous and you need to have a (paid) AWS acc…
Comment: FastQC Quality per tile and per sequence behaving strange after using Cutadapt
by
GenoMax
141k
Check a random sampling of reads to make sure the adapters are being removed. You may also want to blast a few reads to ensure that there i…
Comment: FastQC Quality per tile and per sequence behaving strange after using Cutadapt
by
salias
•
0
Many thanks for your comment. If read-through is happening, shouldn't Cutadapt being taking care of that there? I provided to the Cutadapt …
Traffic: 2523 users visited in the last hour
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by the
version 2.3.6
version 2.3.6