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Showing :
questions
7
votes
5
replies
3.5k
views
What is chr EBV in human sam file?
SAM
Bowtie2
written 6.1 years ago by
salamandra
▴ 550
4
votes
9
replies
6.5k
views
ValueError: invalid literal for int() with base 10: 'start' computeMatrix of deeptools
ChIP-Seq
Deeptools
updated 2.0 years ago by
ronaldgevern
• 0 • written 7.2 years ago by
salamandra
▴ 550
2
votes
3
replies
6.3k
views
STAR alignment error: Genome_genomeGenerate.cpp:208:genomeGenerate: exiting because of *OUTPUT FILE* error: could not create output file ./GenomeDir/
RNA-Seq
STAR
updated 2.3 years ago by
xsmailstop-sub
• 0 • written 6.2 years ago by
salamandra
▴ 550
0
votes
1
reply
2.4k
views
findOverlapsOfPeaks in ChIPpeakAnno R package
R
ChIP-Seq
updated 2.4 years ago by
cvenkat95
▴ 10 • written 7.4 years ago by
salamandra
▴ 550
5
votes
6
replies
26k
views
Error: Encountered internal Bowtie 2 exception (#1) (ERR): bowtie2-align exited with value 1
ChIP-Seq
updated 3.3 years ago by
Levin
▴ 20 • written 7.3 years ago by
salamandra
▴ 550
0
votes
1
reply
2.6k
views
findOverlapsOfPeaks() of package ChIPpeakAnno in R
R
updated 3.5 years ago by
hukai916
▴ 10 • written 7.4 years ago by
salamandra
▴ 550
1
vote
3
replies
1.9k
views
Can we compare peaks of different transcription factors when they were obtained by different criteria (IDR/FDR)?
ChIP-Seq
IDR
RNA-Seq
updated 3.5 years ago by
Biostar
20 • written 6.1 years ago by
salamandra
▴ 550
5
votes
8
replies
2.6k
views
Affymetrix: probeset or transcript cluster?
Affymetrix
microarray
oligo
R
4.0 years ago by
salamandra
▴ 550
2
votes
1
reply
1.3k
views
Summarize by gene for agilent microarray data - one color
R
microarray
limma
updated 4.0 years ago by
Kevin Blighe
88k • written 4.0 years ago by
salamandra
▴ 550
0
votes
2
replies
2.0k
views
Error when reading microarray idat files from illumina
microarray
illumina
illuminaio
R
limma
updated 4.9 years ago by
h.mon
35k • written 4.9 years ago by
salamandra
▴ 550
1
vote
5
replies
3.4k
views
Biopython installation/set path fails in conda env
Biopython
5.0 years ago by
salamandra
▴ 550
1
vote
1
reply
1.7k
views
How to generate a scatter plot of duplication level vs log2reads per kilobase for RNA-seq? (seqmonk)
seqmonk
Duplication level
RNA-Seq
updated 5.2 years ago by
Andrei Poplaukhin
• 0 • written 6.2 years ago by
salamandra
▴ 550
9
votes
4
replies
3.3k
views
mRNA-seq quality report (fastQC): Does it mean samples have adapters and should remove duplicates?
RNA-Seq
fastqc
duplicates
adapters
updated 5.3 years ago by
Biostar
20 • written 6.2 years ago by
salamandra
▴ 550
0
votes
2
replies
3.2k
views
Error in match.arg in boxplot - oligo package
R
oligo
updated 5.3 years ago by
Satyajeet Khare
★ 1.6k • written 7.4 years ago by
salamandra
▴ 550
14
votes
4
replies
6.5k
views
BiomaRt: archive version of ensembl not working
biomart
r
updated 5.6 years ago by
Mike Smith
★ 2.1k • written 5.7 years ago by
salamandra
▴ 550
9
votes
2
replies
17k
views
CPM read count normalization: what does it mean between replicates of same group and within same replicate?
RNA-Seq
Read count normalization
updated 5.7 years ago by
ATpoint
84k • written 5.7 years ago by
salamandra
▴ 550
20
votes
8
replies
8.5k
views
DESeq2 design formula: how to use the opposite interaction term?
deseq2
RNA-Seq
updated 5.7 years ago by
Kevin Blighe
88k • written 5.7 years ago by
salamandra
▴ 550
16
votes
12
replies
14k
views
BioMart: connection fails
BioMart
5.8 years ago by
salamandra
▴ 550
8
votes
9
replies
5.9k
views
How does MACS algorithm work on ATAC-seq data if there is no control sample and no model estimation?
ChIP-Seq
MACS
updated 5.8 years ago by
jihed.chouaref
• 0 • written 6.8 years ago by
salamandra
▴ 550
5
votes
2
replies
2.1k
views
Difference between affy_hg_u133a and with_affy_hg_u133a
R
Biomart
updated 5.9 years ago by
Biostar
20 • written 7.4 years ago by
salamandra
▴ 550
4
votes
8
replies
2.8k
views
Tool that can split genes into groups with similar expression pattern overtime and plot them?
RNA-Seq
clustering
updated 6.0 years ago by
exin
▴ 60 • written 6.0 years ago by
salamandra
▴ 550
1
vote
1
reply
793
views
How to automatically retrieve genes of preselected enzymes in a pathway map?
gene
updated 6.0 years ago by
btsui
▴ 300 • written 6.0 years ago by
salamandra
▴ 550
9
votes
8
replies
3.2k
views
7 follow
Know if a pathway is being functionally activated or repressed by a group of up-regulated genes
RNA-Seq
text-mining
gene set enrichment
updated 6.0 years ago by
Kevin Blighe
88k • written 6.0 years ago by
salamandra
▴ 550
4
votes
14
replies
2.8k
views
Deseq2: What is the most correct experimental design for this experiment?
Deseq2
experimental design
RNA-seq
updated 6.0 years ago by
Devon Ryan
104k • written 6.0 years ago by
salamandra
▴ 550
3
votes
2
replies
6.3k
views
Homer error (findMotifs.pl) : are you sure you chose the right length for motif finding?
Chip-seq
motifs
homer
updated 6.1 years ago by
Kevin Blighe
88k • written 6.1 years ago by
salamandra
▴ 550
4
votes
4
replies
2.2k
views
How to mask motif in HOMER?
mask motif
chip-seq
homer
updated 6.1 years ago by
Kevin Blighe
88k • written 6.1 years ago by
salamandra
▴ 550
3
votes
14
replies
5.1k
views
How to use a specific promoter set on findMotifs.pl of HOMER?
ChIP-Seq
HOMER
promoter set
motifs
updated 6.1 years ago by
Kevin Blighe
88k • written 6.1 years ago by
salamandra
▴ 550
0
votes
0
replies
1.8k
views
ChIP-seq: To use IDR, samples have to pass both tests or is it enough to pass the pseudo-replicate test?
ChIP-Seq
IDR
6.1 years ago by
salamandra
▴ 550
4
votes
3
replies
1.3k
views
Why when splitting file in two we add 1 line before dividing the number of lines by 2?
split
commandline
bash
6.1 years ago by
salamandra
▴ 550
2
votes
4
replies
3.8k
views
RNA-seq: Should remove duplicates in all samples of same experiment although some do not have technical duplicates?
RNA-Seq
duplication
dupRadar
updated 6.1 years ago by
h.mon
35k • written 6.1 years ago by
salamandra
▴ 550
3
votes
1
reply
2.4k
views
How to calculate effective genome size using unique-kmers.py from khmer program?
effective genome size
khmer
ChIP-Seq
6.1 years ago by
salamandra
▴ 550
1
vote
1
reply
1.9k
views
What parameter values to change in peak calling with MACS2 when calculating IDR afterwards?
ChIP-Seq
MACS2
IDR
6.1 years ago by
salamandra
▴ 550
2
votes
4
replies
1.5k
views
Does Bowtie2 index use haplotypes and patches? and is the reference genome of Bowtie2 from UCSC or ensembl?
ChIP-Seq
haplotypes
scaffolds
Bowtie2
ensembl
updated 6.1 years ago by
Devon Ryan
104k • written 6.1 years ago by
salamandra
▴ 550
0
votes
0
replies
1.8k
views
What tool should be used to determine IDR or Np/Nt and N1/N2 between ChIP-seq samples?
ChIP-Seq
IDR
ENCODE
6.1 years ago by
salamandra
▴ 550
1
vote
1
reply
2.9k
views
How to select a gene annotation file in ensembl that contain un-localized scaffolds, but NO patches or haplotypes?
assembly
RNA-Seq
STAR
genome
6.1 years ago by
salamandra
▴ 550
3
votes
1
reply
1.7k
views
RNA-seq: Can we obtain distances between samples of different experiments?
RNA-Seq
sample clustering
updated 6.1 years ago by
h.mon
35k • written 6.1 years ago by
salamandra
▴ 550
11
votes
4
replies
5.1k
views
Questions regarding VST/rlog correction to plot sample distances between RNA-seq samples
RNA-Seq
R
DEseq
updated 6.1 years ago by
Kevin Blighe
88k • written 6.1 years ago by
salamandra
▴ 550
5
votes
2
replies
1.9k
views
Does importing HTseqcounts with txtimport corrects for gene length?
RNA-Seq
deseq
R
updated 6.1 years ago by
Devon Ryan
104k • written 6.1 years ago by
salamandra
▴ 550
3
votes
13
replies
9.1k
views
Mark duplicates PICARD error: Unable to access jarfile 'MarkDuplicates.jar' with commandline/with R dupRadar package
markduplicates
Picard
RNA-Seq
6.2 years ago by
salamandra
▴ 550
2
votes
1
reply
873
views
Seqmonk has two CRCh38 human genome versions, which one is the major release?
assembly
RNA-seq
genome
ensembl
6.2 years ago by
salamandra
▴ 550
4
votes
6
replies
6.3k
views
STAR ERROR: could not open genomeParameters.txt / How to increase RAM?
STAR
RNA-Seq
6.2 years ago by
salamandra
▴ 550
2
votes
4
replies
1.4k
views
Which gene annotation file in UCSC is equivalent to the gene annotation file that is NOT 'abinitio' or 'chromossome' in ensembl?
RNA-Seq
anotation file
6.2 years ago by
salamandra
▴ 550
16
votes
14
replies
18k
views
Which truseq trimmomatic adapters file to use when removing truseq adapters?
adapters
trimmomatic
RNA-seq
6.2 years ago by
salamandra
▴ 550
10
votes
11
replies
5.7k
views
Does adapter removal (with trimmomatic) also remove barcodes?
barcodes
adapters
RNA-Seq
updated 6.2 years ago by
swbarnes2
14k • written 6.2 years ago by
salamandra
▴ 550
1
vote
3
replies
2.4k
views
Error: could not find function "exptData"
R
updated 6.3 years ago by
mhan
• 0 • written 7.4 years ago by
salamandra
▴ 550
3
votes
6
replies
3.4k
views
Isn't fastq file header suppose to have barcode sequence?
ChIP-Seq
Quality control
updated 6.4 years ago by
Biostar
20 • written 6.5 years ago by
salamandra
▴ 550
20
votes
11
replies
9.4k
views
6 follow
If we use MACS2 do we need to remove duplicate sequences with samtools rmdup ?
ChIP-Seq
Duplicates
MACS2
updated 6.5 years ago by
Biostar
20 • written 6.5 years ago by
salamandra
▴ 550
0
votes
0
replies
1.3k
views
Beedtools intersect: more peaks intersected than intersected portions of those peaks?
beedtools intersect
sequencing
RNA-seq
ChIP-seq
6.5 years ago by
salamandra
▴ 550
3
votes
7
replies
5.4k
views
Why command gives the error: extra operand 23243?
commandline
Bash
comm
6.5 years ago by
salamandra
▴ 550
1
vote
4
replies
4.5k
views
Deeptools - ComputeMatrix: Why does 'referencePoint center' gives the same heatmap as 'referencePoint TSS'
ChIP-Seq
sequencing
deeptools
6.5 years ago by
salamandra
▴ 550
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