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Forum: Site Use Guidelines
meta biostars Guidelines
updated 17 days ago by 43k • written 14.6 years ago by 100k
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Forum: Bioinformatician who was retaliated against after reporting discriminatory harassment - Please help me navigate getting out of the situation I'm in
Career advice
updated 25 days ago by ★ 2.5k • written 26 days ago by • 0
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Forum: Ideal PC configurations and operating system for bioinformatics laboratory
PC
8 days ago by Estevão • 0
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Forum: VCF bulk handling
bcftools SnpEff WGS VEP VCF
23 days ago by Javier • 0
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Forum: Bioinformatics: Where do I start
Sequencing
updated 24 days ago by 43k • written 24 days ago by ▴ 10
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Forum: Improvement on automatic annotation of single-cell RNA-seq data from the literature using LLM
annotation scRNA llm
17 days ago by yxwucq • 0
6 results • Page 1 of 1
Recent Votes
Comment: Tassel 5 GBS: Is there something wrong with my fastqs?
Answer: what is another word for a BLAST "hit"?
Comment: what is another word for a BLAST "hit"?
Comment: what is another word for a BLAST "hit"?
Comment: How can I merge GFF files together to produce a file with gene functions from bo
Comment: Contig assembly task, errors
Comment: Where to get the following bed file?
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Recent Replies
Answer: Is it Possible to "Merge" data from Replicates within a VCF SNP File by Pierre Lindenbaum 161k
use bcftools merge with --force-samples and then use jvarkit https://jvarkit.readthedocs.io/en/latest/VcfFilterJdk/ to replace the no-call …
Answer: In IGV is this a heterogeneous mutation or false call? by swbarnes2 14k
Since all the reads that show the alternate allele have to be clipped to align there, I'd say those reads are misaligned. I would say that…
Comment: Tassel 5 GBS: Is there something wrong with my fastqs? by GenoMax 142k
> Here's a few lines from the head of the raw fastq If your files are actually compressed (as their names suggest) then you should not be …
Comment: Tassel 5 GBS: Is there something wrong with my fastqs? by GenoMax 142k
If you added the `\` before the `+` then there is no need for it. It makes it appear that the sequences are not in proper fastq format. Ple…
Comment: Tassel 5 GBS: Is there something wrong with my fastqs? by Mensur Dlakic ★ 27k
This seems to be the same unanswered question as before, except that its description is split over two threads. You are now forcing us to r…
Answer: what is another word for a BLAST "hit"? by dec986 ▴ 380
I'll probably go with "matches" even though it doesn't sound as good as just "hit"
Comment: what is another word for a BLAST "hit"? by bk11 ★ 2.4k
> hits from BLAST hits sequences
Comment: what is another word for a BLAST "hit"? by Pierre Lindenbaum 161k
> is there a synonym for hits from BLAST? alignment
Comment: How are score_weights calculated in this code? by Ram 43k
Is this ALL the context you can give us?
Comment: Genome Assembly task + Protein Translation, assignment advice on a question by Ram 43k
Where did you copy-paste this from? It looks like an assignment question.
Comment: Contig assembly task, errors by rackbersingh • 0
Hi Phillip, Thanks for sharing you knowledge and funnily enough this really was an amplitude test, but anyways your sharing of knowledge h…
Comment: Can I readmap short reads to rDNA references? by GenoMax 142k
If these are short reads and the organisms are related then you are going to have a difficult time classifying the reads.
Comment: extract viral protein of interest from 10k whole viral genomes by GenoMax 142k
With a large dataset it is always best to do the search locally (or in the cloud, if you don't have necessary hardware available locally).
Comment: Truncated metadata file report from ENA Portal API by Giulia • 0
Thank you so much for your hint! I'll look into this!
Comment: How to convert Haps file to vcf file? by curious ▴ 750
unzip haps then: bin/shapeit \ -convert \ --input-haps {haps_prefix} \ --thread {cpus} \ --output-…
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