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21 results • Page
1 of 1
Sort: Votes
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Votes
Replies
4
votes
7
replies
462
views
RNA seq analysis
DESeq
RNA-seq
8 hours ago by
Jacek
▴ 10
1
vote
4
replies
328
views
Getting the coding_sequence.fasta from the .gff file from the AUGUSTUS gene prediction.
augustus
annotation
assembly
genome
updated 16 hours ago by
Juke34
8.6k • written 2 days ago by
Vijith
▴ 30
1
vote
1
reply
127
views
sci-RNA-seq
Seurat
sci-RNA-seq
updated 23 hours ago by
Ram
43k • written 1 day ago by
kilcdincer
▴ 10
0
votes
0
replies
65
views
Why the shape of RCS is contradictory to the p value for nonlinearity.
RCS
ggplot2
ggplot
R
updated 14 hours ago by
GenoMax
142k • written 15 hours ago by
Dude
• 0
0
votes
1
reply
111
views
Polish a large genome with Pilon
Pilon
Pacbio
Polish
Illumina
13 hours ago by
sansan_96
▴ 90
0
votes
2
replies
153
views
Using Cutadapt to trim adapters from paired-end small RNA sequence data
trimming
adapters
cutadapt
sRNA-seq
smallRNA
9 hours ago by
melissa.joubert
• 0
0
votes
0
replies
50
views
ReactomeGSA
ReactomeGSA
9 hours ago by
Shaimaa Gamal
▴ 10
0
votes
3
replies
97
views
Problem with Calling Variants from RNA-Seq data
vcf
gatk
benchmark
giab
rna-seq
6 hours ago by
Esraa
• 0
0
votes
1
reply
88
views
bbmerge (bbmap) ~ error with insert size file output
bbtools
bbmerge
bbmap
updated 5 hours ago by
GenoMax
142k • written 11 hours ago by
chrisk
• 0
0
votes
1
reply
98
views
DEG analysis with limma and contrast matrix using multiple Parkinson's cohorts in whole blood: is it normal to always get NS?
rstudio
DEG
limma
updated 5 hours ago by
ATpoint
82k • written 6 hours ago by
egascon
• 0
0
votes
1
reply
92
views
perseus software (version 1.5.5)
preseus
mass
spectrometry
data
updated 3 hours ago by
Joe
21k • written 13 hours ago by
iqra
• 0
0
votes
0
replies
38
views
Difference in number of reads when using duplex and simplex base calling with Dorado
base
calling
nanopore
simplex
dorado
duplex
3 hours ago by
Lélé
• 0
0
votes
0
replies
46
views
Seurat v5 and how to correctly integrate across multiple experiments
Seurat
scRNA
R
updated 2 hours ago by
ATpoint
82k • written 3 hours ago by
yura.grabovska
▴ 70
0
votes
0
replies
23
views
Is it okay to assemble my 12 metatranscriptome samples with Trinity separately?
trinity
metatranscriptome
1 hour ago by
jway
• 0
0
votes
0
replies
17
views
In one PCA plot, can I calculate the percentage of different factors that contribute to the PCA?
PCA
R
VARIANCE
1 hour ago by
diqixiaoyaoer
▴ 10
0
votes
1
reply
27
views
Wrong ZYGOSITY by ensembl vep on DeepVariant vcf file
annotation
vcf
vep
zygosity
deepvariant
1 hour ago by
asalimih
▴ 60
0
votes
1
reply
29
views
Can 5' and 3' scRNAseq be processed with the same pipeline?
singlecell
seurat
10XGenomics
scRNAseq
updated 32 minutes ago by
yura.grabovska
▴ 70 • written 1 hour ago by
ev97
▴ 20
0
votes
2
replies
21
views
Blastn error : ncbi::CObject::ThrowNullPointerException() - Attempt to access NULL > pointer.
blast
meta
how-to
ncbi
forum
updated 3 minutes ago by
GenoMax
142k • written 57 minutes ago by
hophuquy0944
• 0
0
votes
0
replies
1
view
DOCK6 installation on MacOS M1
installation
troubleshooting
DOCK6
just now by
Rodolfo Adrián
• 0
0
votes
1
reply
137
views
Why does assigning genes with biomart give me different values than using a transcripts_to_genes.txt file?
biomart
RNAseq
kallisto
updated 22 hours ago by
GenoMax
142k • written 22 hours ago by
bioinfo
▴ 150
0
votes
0
replies
61
views
NEURON, Brian2, NEST, or PyNN
NEST
NEURON
PyNN
Brian2
15 hours ago by
brunofelicianodeomena
• 0
21 results • Page
1 of 1
Recent Votes
Answer: Genotyping sites with N in reference genome
A: how to combine RNA seq data from 4 lanes
Answer: Cannot install bwa-mem2 via conda
Answer: Sub-sampling a BAM to a fixed number of reads
Comment: Downsample BAM file to specific amount of reads
Answer: Long reads and fixing of mate-pair issues/marking duplicates with samtools
Answer: Long reads and fixing of mate-pair issues/marking duplicates with samtools
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Recent Replies
Comment: Blastn error : ncbi::CObject::ThrowNullPointerException() - Attempt to access NU
by
GenoMax
142k
Did you remeber to unzip/untar the `nt` preformatted files that you downloaded?
Comment: Genotyping sites with N in reference genome
by
shpak.max
▴ 50
Just to clarify, I have set additional sites in the reference genome to 'N', which means (based on your statement) that these will not be g…
Comment: Blastn error : ncbi::CObject::ThrowNullPointerException() - Attempt to access NU
by
Pierre Lindenbaum
161k
see https://www.biostars.org/p/9496674/ <!-- when i runed blastn(version 2.15) and nt database(latest version). I used aspera to download…
Answer: Genotyping sites with N in reference genome
by
Pierre Lindenbaum
161k
> Does UnifiedGenotyper skip/ignore sites where the reference genotype is N yes. The variant is in YOUR data, not in the reference where '…
Comment: Can 5' and 3' scRNAseq be processed with the same pipeline?
by
yura.grabovska
▴ 70
Do you have samples that should in theory have a good degree over overlap in terms of cell types across the two sequencing types? I would …
Comment: Wrong ZYGOSITY by ensembl vep on DeepVariant vcf file
by
asalimih
▴ 60
I found out that vep is only checking the GT field therefore assigning HOM to the Zygosity. Therefore the problem should be from deepvarian…
Comment: Add stats to boxplot in R
by
Lélé
• 0
To remove "Kruskall Wallis" you can try adding the argument label="p" or label = "p.signif" which displays the p-value or *** like this: …
Answer: Sequencing Depth (Read Depth) Calculations
by
GenoMax
142k
You could also use `pandepth` ([**LINK**][1]) or `mosdepth` ([**LINK**][2]) as well. These will allow you to be flexible about windowed cov…
Comment: Sequencing Depth (Read Depth) Calculations
by
Pierre Lindenbaum
161k
it looks looks ok to me. <!-- This cmd works and gives me 40.5235, I was wondering whether this cmd is right or wrong, and does anyone hav…
Comment: Add stats to boxplot in R
by
Ghada
• 0
Great. thats work. Do you know how can I remove the kruskall-Wallis name from the plot? Also I would like to draw a line under the kruskall…
Comment: Using ggplotly in R
by
jared.andrews07
★ 16k
Again, this is not particularly difficult to do with [plotly itself](https://plotly.com/r/line-and-scatter/#adding-color-and-size-mapping) …
Comment: Duplicated reads (IDs) from nanopore sequencing
by
GenoMax
142k
If you only have supplementary alignments then they may be caused by reasons mentioned here --> https://www.biostars.org/p/308853/ I did no…
Comment: Cannot install bwa-mem2 via conda
by
Pierre Lindenbaum
161k
> Please do not paste screenshots of plain text content, it is counterproductive. You can copy paste the content directly here (using the c…
Answer: Cannot install bwa-mem2 via conda
by
Istvan Albert
100k
The problem is caused by running the ARM-based conda. Bioinformatics software is compiled for Intel based CPUs only. Uninstall that versio…
Comment: Duplicated reads (IDs) from nanopore sequencing
by
marco.barr
▴ 110
[https://www.biostars.org/p/9559284/][1] you could try this [1]: https://www.biostars.org/p/9559284/
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