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27 results • Page
1 of 1
Sort: Views
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Views
Votes
Replies
0
votes
2
replies
2.7k
views
demultiplexing with guppy_basecaller and guppy_barcoder using --detect_mid_strand_adapter --detect_mid_strand_barcodes produce > 90% of unclassified …
demultiplexing
guppy
15 hours ago by
andres.firrincieli
3.2k
2
votes
1
reply
2.1k
views
Question about PLINK allelic scoring (PRS)
plink
PRS
updated 12 hours ago by
Ram
38k • written 4.6 years ago by
wonseok.cchoi
• 0
0
votes
2
replies
1.4k
views
Differences in RNAseq Variant Calling and Allele Specific Expression
RNA-Seq
variant calling
allele specific expression
updated 46 minutes ago by
afei
• 0 • written 5.1 years ago by
serpalma.v
▴ 70
1
vote
3
replies
456
views
Using Copy Number Alterations detected in other studies for the same tumor cell line
genomes
cancer
CNV
variants
CNA
updated 5 hours ago by
lethalfang
▴ 90 • written 4 months ago by
ashenflower
▴ 20
0
votes
6
replies
378
views
Kallisto bustools for scRNA-seq
Kallisto
updated 5 hours ago by
dsull
★ 4.0k • written 2 days ago by
t.ru
▴ 20
2
votes
4
replies
360
views
FeatureCounts >edgeR > GO
RNA-SEQ
11 hours ago by
Pegasus
▴ 80
0
votes
2
replies
230
views
How to limit the use of RAM for clustalo?
Linux
Clustalo
RAM
14 hours ago by
animeshizik
• 0
1
vote
4
replies
223
views
Artificial reads - remove multiple mapped reads against reference genome, and only keep reads that completely match without any mismatches - samtools
samtools
18 hours ago by
Agamemnon
▴ 60
1
vote
2
replies
169
views
Snakemake doesn't recognize output files even though they are created
python
Snakemake
9 hours ago by
DdogBoss
• 0
1
vote
4
replies
165
views
Suggestion for approaching multiple conditions scRNA-seq
scRNA-seq
12 hours ago by
Eisuan
▴ 20
1
vote
3
replies
149
views
mpileup to sync file
AF
samtools
selection
vcf
popoolation
updated 19 hours ago by
Pierre Lindenbaum
153k • written 20 hours ago by
qstefano
▴ 20
2
votes
2
replies
135
views
STAR solo set parameters for scrna-seq
solo
STAR
17 hours ago by
t.ru
▴ 20
0
votes
0
replies
115
views
How to export GWAS lists available in Open Targets database
GWAS
gene
openTargets
16 hours ago by
dzisis1986
▴ 60
0
votes
1
reply
113
views
Quality control of Chip-seq data (NRF and PCB)
control
library
quality
PCB
Chip-seq
NRF
updated 12 hours ago by
Darked89
4.2k • written 20 hours ago by
michelafrancesconi9
• 0
1
vote
2
replies
104
views
Analysing the Effect of a Drug on the Morphological Changes of a Cell Type
drug-effect
statistical-analysis
5 hours ago by
E-HR
• 0
0
votes
0
replies
100
views
Nucleotide substitution model and neighbor-joining tree
phylogeny
substitution
mega
neighbor-joining
modeltest
18 hours ago by
poecile.pal
▴ 50
0
votes
2
replies
98
views
using CITE-seq data to cluster single cells
clustering
cite-seq
k-means
10x
8 hours ago by
chi.delta
▴ 40
3
votes
1
reply
89
views
Why not use ONLY promoter-bound peaks when testing for enrichment in differentially-bound regions?
ChIP-seq
ChIPseeker
GO
updated 9 hours ago by
Ram
38k • written 11 hours ago by
e.r.zakiev
▴ 30
1
vote
1
reply
82
views
Best way to visualize .paf or .sam alignment to a fasta file
visualization
sam
fasta
paf
alignment
updated 6 hours ago by
GenoMax
127k • written 8 hours ago by
Mark
• 0
0
votes
0
replies
79
views
GO analysis using diamond blastp output
blastp
GO
analysis
Diamond
22 hours ago by
hellokwmin
• 0
1
vote
1
reply
76
views
Integration of RNA seq data aligned to different reference genome versions
scRNA-seq
RNA-seq
updated 11 hours ago by
Ram
38k • written 12 hours ago by
susibing
▴ 20
0
votes
0
replies
57
views
Clustering method for CT values of two group
PCA
Clustering
bioconductor
R
12 hours ago by
Maria17
▴ 10
0
votes
0
replies
56
views
Problem when downloading dataset from ArrayExpress
Bioconductor
ArrayExpress
8 hours ago by
cogen859
• 0
0
votes
0
replies
53
views
ABSOLUTE for tumour purity with WES
tumour-purity
ABSOLUTE
WES
updated 13 hours ago by
Ram
38k • written 13 hours ago by
Cvlind
• 0
0
votes
0
replies
52
views
Extract cluster genes
fasta
extract
cluster
9 hours ago by
BATMAN
• 0
0
votes
0
replies
52
views
UK BioBank HLA imputation
ukbb
hla
imputation
12 hours ago by
hla_help
• 0
0
votes
0
replies
10
views
topGO error :KS elim test
KS
topGO
enrichment
-elim
analysis
40 minutes ago by
Priya Rao
• 0
27 results • Page
1 of 1
Recent Votes
Answer: How can I use bcftools mpileup or an alternative to find ALL variants without an
Answer: What is the NCBI's definition of an "atypical genome"?
Comment: GO enrichment analysis
Comment: GO enrichment analysis
Answer: What is the NCBI's definition of an "atypical genome"?
A: Why is RNA-Seq or cDNA used to detect gene fusions instead of gDNA?
Answer: Somatic truth set
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Bogdan
★ 1.3k
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pavelasquezv
▴ 50
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▴ 550
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Recent Replies
Answer: Align miRNA library (small RNA-seq) without trimming
by
Ming Tommy Tang
★ 2.9k
bowtie 1 is good for short read < 50bp, how long is your read? bowtie2 is better for reads > 50 bp
Comment: Differences in RNAseq Variant Calling and Allele Specific Expression
by
afei
• 0
Hi! I'm also confused by this. And the site (https://gatkforums.broadinstitute.org/gatk/categories/gatk-support-forum) is closed. Have you …
Comment: Variant caller reports a homozygous variant genotype, but more reads are associa
by
rebeliscu
▴ 50
Indeed, I thought the quality might be a factor. Still, given the information, it seems odd to me that that call was made. Thanks for you…
Answer: How can I use bcftools mpileup or an alternative to find ALL variants without an
by
cfos4698
▴ 730
To generate a VCF file one would normally pipe the input of an `mpileup` command into an actual `call` command. For example: ``` bcftools …
Answer: PTM-aware protein folding / docking
by
Mensur Dlakic
★ 23k
Your assumption is correct - AlphaFold pTM has nothing to do with post-translational modifications. See [**here**][1]. Not to discourage…
Answer: Admixture cv error
by
Declan
• 0
I had this issue today and was able to resolve it by changing the random seed Admixture uses by adding `-s time` (which sets the random see…
Answer: Total No of Genes of GENCODE Release 43
by
ahmad
• 0
The following is the correct code gtf_43<-rtracklayer::import('gencode.v43.primary_assembly.annotation.gtf') dtgtf_43<-data.frame(gtf_4…
Comment: Total No of Genes of GENCODE Release 43
by
ahmad
• 0
Hello, thanks for replying, by mistake I paste the wrong code. the following is the corrected gtf_43<-rtracklayer::import('gencode.v43…
Comment: Kallisto bustools for scRNA-seq
by
dsull
★ 4.0k
I have no idea what that is besides a bunch of jumbled sequences. You'll need look up the structure of the reads / library from wherever yo…
Comment: Aberrant splicing in bulk RNAseq
by
dsull
★ 4.0k
First thing: kallisto cannot interface with UMI-tools (kallisto doesn't use read headers and therefore you're stuck with "the kallisto way"…
Comment: Using Copy Number Alterations detected in other studies for the same tumor cell
by
lethalfang
▴ 90
There is some somatic SV study on these HCC1395: https://doi.org/10.1186/s13059-022-02816-6. There is also a CNA preprint from years ago b…
Comment: Somatic truth set
by
lethalfang
▴ 90
Just in case things are hard to find, for the WGS/WES, these are the data (what the file names mean: https://sites.google.com/view/seqc2/ho…
Comment: How to get subset of a Seurat object based on metadata?
by
jv
★ 1.0k
Hmm, not sure why `subset` doesn't match to '0:CD8 T cell' but maybe it's the ":"? An alternative option is the following: ``` idx <- whi…
Comment: GO enrichment analysis
by
Jeremy
▴ 780
g:Profiler has an Arabidopsis option: [g:Profiler][1] [1]: https://biit.cs.ut.ee/gprofiler/gost
Answer: Any tips in landing a bioinformatic job?
by
Carambakaracho
★ 3.2k
The first job is a nightmare and you will need some luck. I understand you're looking for an industry offering. Unfortunately I've worked o…
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