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Comment: 4 Fastq files for a single run generated by 10X
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hkarakurt
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Thank you for your detailed answer. I will check the reads and compare them with chemistry manual as you suggested.
Thank you again for yo…
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Thank you @atpoint for your response. However, aim of analysing to generate a matrix with all dataset available after batch correction.
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Agreed. I would just force the cells to 5000 or so, and then proceed with the normal QC. If there's a lot of trash "cells" in the dataset t…
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you should use the `wild cards` to download all the given above GCF files. Moverover, you can use the commands of `curl` , `wget` and `slow…
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This has been asked so many times before. Analyse them separately, then make a statement on reproducibility of results using a meta-analysi…
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What is qcovhsp (high scoring segment pair) in BLAST output. Can we use this parameter to calculate the gene coverage?
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Scaling the data will give you Z-scores. This is a standard way of preparing data for PCA. I would also suggest trying a center-log-ratio…
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Thank you for your answer,but after verifying the protein file, it seems there is no problem. Even if there are missing codons, the program…
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cmdcolin
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i can't really tell you why your code doesn't work if you dont provide it. and, if you describe as much as possible what your goals are, th…
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MatthewP
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maybe try?:
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Looks like you have corrupt or improperly-formatted input. It's expecting to parse a floating-point number where the word "score" appears.…
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dsull
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The knee plot is just one type of QC. I'd say just proceed with your analysis and see if you observe anything funky (analysis itself is a Q…
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