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questions
0
votes
4
replies
1.9k
views
Ran dipSpades for a diploid fungus genome -- now what?
diploid
genome assembly
dipspades
updated 3.6 years ago by
Biostar
20 • written 6.6 years ago by
Lina F
▴ 200
3
votes
11
replies
16k
views
SPAdes is running out of memory
spades
hybrid
assembly
fungi
memory
updated 3.7 years ago by
carisdak
• 0 • written 6.7 years ago by
Lina F
▴ 200
0
votes
3
replies
2.7k
views
SPAdes error code -6
SPAdes
metagenomics
Assembly
4.8 years ago by
Lina F
▴ 200
2
votes
4
replies
2.2k
views
How does bbduk calculate it's memory requirements?
bbduk
docker
memory
4.9 years ago by
Lina F
▴ 200
2
votes
5
replies
1.7k
views
Error building snpEff database "Transcript 'hypothetical_protein' already exists"
snpeff
database
build
updated 13 months ago by
Jane
• 0 • written 5.8 years ago by
Lina F
▴ 200
2
votes
6
replies
2.7k
views
Running two bedtools commands in series produces error "has non positional records"
bedtools
vcf
5.8 years ago by
Lina F
▴ 200
3
votes
2
replies
3.3k
views
Jellyfish: every other kmer count is zero
kmer
counting
jellyfish
genome size estimation
5.9 years ago by
Lina F
▴ 200
0
votes
4
replies
2.8k
views
Visualizing whole genome alignment beyond dotplots
dotplot
visualization
alignment
whole genome
updated 6.2 years ago by
h.mon
35k • written 6.2 years ago by
Lina F
▴ 200
4
votes
2
replies
4.2k
views
Removing common SNPs from a set of VCF files
SNP
vcf
filtering
6.5 years ago by
Lina F
▴ 200
1
vote
3
replies
4.6k
views
How to filter SNPs after Freebayes, if at all, in a fungal haploid genome alignment?
freebayes
SNP
filtering
6.6 years ago by
Lina F
▴ 200
3
votes
4
replies
3.4k
views
Align two microbial genomes -- is mummer still the way to go?
genome alignment
mummer
nucmer
snps
vcf
6.7 years ago by
Lina F
▴ 200
0
votes
0
replies
1.3k
views
Upcoming workshops tackling data analysis of hybrid sequencing technologies?
nanopore
minion
workshop
hybrid
sequencing
6.7 years ago by
Lina F
▴ 200
6
votes
9
replies
8.0k
views
How to get fasta sequences of features from a GFF3 file?
gff3
fasta
parser
updated 6.8 years ago by
Matt Shirley
10k • written 6.8 years ago by
Lina F
▴ 200
1
vote
8
replies
5.0k
views
Demultiplexing reads from two libraries (single and dual indices) that were sequenced in the same sequencing run
demultiplexing
nextseq
bcl2fastq
updated 6.8 years ago by
h.mon
35k • written 6.8 years ago by
Lina F
▴ 200
6
votes
5
replies
4.7k
views
How to assemble complex soil metagenome datasets?
metagenomics
assembly
soil
updated 6.8 years ago by
Joe
21k • written 6.8 years ago by
Lina F
▴ 200
0
votes
1
reply
1.3k
views
Capturing whole gene sequences from WGS metagenomics sample
metagenomics
assembly
contigs
binning
updated 6.9 years ago by
Sej Modha
5.3k • written 6.9 years ago by
Lina F
▴ 200
0
votes
0
replies
2.4k
views
Statistical approach to identifying outliers in RNAseq data?
RNA-Seq
outliers
detection
statistics
7.0 years ago by
Lina F
▴ 200
6
votes
2
replies
3.2k
views
Calling SNPs with samtools: how to specify how many supporting observations are required?
samtools
variant calling
snps
freebayes
updated 7.0 years ago by
Brian Bushnell
20k • written 7.0 years ago by
Lina F
▴ 200
0
votes
2
replies
1.2k
views
RNAseq transcript quantification: how to deal with different number of genes in host organism?
RNA-Seq
sailfish
quantification
transcripts
R
updated 7.0 years ago by
h.mon
35k • written 7.0 years ago by
Lina F
▴ 200
1
vote
4
replies
2.3k
views
Best reference database for 16S soil datasets?
metagenomics
soil
reference
16S
updated 7.1 years ago by
Denise CS
★ 5.2k • written 7.1 years ago by
Lina F
▴ 200
1
vote
4
replies
1.6k
views
How to assess biases in ribosomally depleted RNAseq data?
RNA-Seq
bias
transcripts
7.2 years ago by
Lina F
▴ 200
0
votes
0
replies
1.7k
views
How does RNA-SeQC calculate the "Mean CV" value?
RNA-Seq
qc
coefficient of variation
7.2 years ago by
Lina F
▴ 200
0
votes
0
replies
2.5k
views
How to format input data for Picard CollectRnaSeqMetrics
picard
RNA-Seq
gtf
updated 7.3 years ago by
Pierre Lindenbaum
161k • written 7.3 years ago by
Lina F
▴ 200
0
votes
2
replies
1.9k
views
RNA-SeQC does not calculate information about "Strand Specificity"
RNA-Seq
qc
updated 7.3 years ago by
Charles Warden
8.2k • written 7.3 years ago by
Lina F
▴ 200
3
votes
2
replies
3.2k
views
Yeast GTF file needed for RNA-SeQC run
qc
gtf
yeast
RNA-Seq
RNA-SeQC
updated 7.3 years ago by
apa@stowers
▴ 600 • written 7.3 years ago by
Lina F
▴ 200
4
votes
6
replies
6.1k
views
bwa mem: which parameters to tune to improve alignment?
bwa
parameters
tuning
7.4 years ago by
Lina F
▴ 200
1
vote
2
replies
3.0k
views
Generating consensus sequences
consensus
fasta
bwa
vcftools
updated 7.4 years ago by
vmicrobio
▴ 290 • written 7.4 years ago by
Lina F
▴ 200
0
votes
1
reply
1.6k
views
FreeBayes does not deal with ambiguous nucleotides -- what are good alternatives?
freebayes
open source
SNP
7.5 years ago by
Lina F
▴ 200
6
votes
6
replies
8.4k
views
Cannot find downloaded files from SRA toolkit (prefetch)
prefetch
sratoolkit
updated 10 months ago by
Ram
43k • written 7.5 years ago by
Lina F
▴ 200
1
vote
2
replies
1.4k
views
Do I need to explicitly remove rRNA from E. coli RNAseq data?
rrna
rnaseq
ecoli
transcriptomics
7.5 years ago by
Lina F
▴ 200
0
votes
2
replies
4.1k
views
How to demultiplex 16S PE Miseq reads?
miseq
demultiplex
fastq
16S
paired end reads
7.8 years ago by
Lina F
▴ 200
0
votes
1
reply
1.4k
views
Microbiome data: Should unmatched reads be included in Shannon Diversity calculations?
microbiome
shannon diversity
unmatched reads
8.2 years ago by
Lina F
▴ 200
2
votes
1
reply
3.6k
views
Extracting more features from EMBL files with Biopython
embl
biopython
updated 18 months ago by
Ram
43k • written 8.8 years ago by
Lina F
▴ 200
2
votes
3
replies
2.3k
views
How to incorporate metadata in 16S amplicon study and identify confounding variables
amplicon
confounders
16S
updated 16 months ago by
Ram
43k • written 8.9 years ago by
Lina F
▴ 200
8
votes
8
replies
4.4k
views
Is the order of records in the bam file produced by bwa the same as the order in the input fastq file?
mem
fastq
bwa
aln
sampe
updated 3.1 years ago by
Ram
43k • written 9.5 years ago by
Lina F
▴ 200
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