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3
votes
7
replies
1.1k
views
Is removing rRNAs, tRNAs, etc really a necessary step in the miRNA analysis?
mirdeep2 miRNA RNA-seq
updated 22 days ago by ▴ 20 • written 21 months ago by ▴ 160
5
votes
4
replies
1.8k
views
How to subset the output of tximport in DE analysis using DEseq2
DEseq2 tximport rna-seq r
updated 12 months ago by 68k • written 2.6 years ago by ▴ 160
2
votes
12
replies
3.8k
views
How to get KEGG pathway names in KEGG enrichment analysis using clusterprofiler
annotation enrichment KEGG clusterprofiler RNA-Seq
updated 17 months ago by ★ 2.6k • written 2.8 years ago by ▴ 160
0
votes
0
replies
292
views
how to annotate mapping peaks within a defined genomic region？
piRNA genome RNA-seq annotation
20 months ago by tianshenbio ▴ 160
0
votes
0
replies
655
views
Comment: Comment: How to merge genomic features shared by more than one samples
20 months ago by tianshenbio ▴ 160
0
votes
1
reply
655
views
Comment: Comment: How to merge genomic features shared by more than one samples
21 months ago by tianshenbio ▴ 160
0
votes
4
replies
655
views
How to merge genomic features shared by more than one samples
bedtools gene RNA-seq bed annotation
20 months ago by tianshenbio ▴ 160
0
votes
1
reply
769
views
Convert a DNA sequence to MEME format
FIMO fasta motif MEME-suite format conversion
updated 22 months ago by 125k • written 22 months ago by ▴ 160
1
vote
0
replies
1.4k
views
Comment: Comment: How to select genes with multi-exon 3'UTR
22 months ago by tianshenbio ▴ 160
8
votes
6
replies
1.4k
views
How to select genes with multi-exon 3'UTR
rna-seq genome gff gene
updated 22 months ago by 7.5k • written 22 months ago by ▴ 160
1
vote
0
replies
2.1k
views
Functional annotation(GO,KEGG): Blast2GO+KAAS or EggNOG
blast2go eggnog kegg rna-seq genome
updated 23 months ago by 20 • written 2.8 years ago by ▴ 160
0
votes
1
reply
2.5k
views
Comment: C: Why sortmerna 4.2.0 runs so slow?
23 months ago by tianshenbio ▴ 160
2
votes
8
replies
2.5k
views
Why sortmerna 4.2.0 runs so slow?
RNA-Seq sortmerna software error RNA rna-seq
updated 23 months ago by ▴ 20 • written 2.8 years ago by ▴ 160
2
votes
3
replies
2.6k
views
How to get a list of all KEGG ko terms vs names?
kegg annotation rna-seq
updated 15 months ago by ▴ 10 • written 2.8 years ago by ▴ 160
8
votes
12
replies
9.2k
views
6 follow
Clusterprofiler error: No gene can be mapped
RNA-seq GO enrichment R GO clusterProfiler
updated 21 months ago by ▴ 20 • written 2.8 years ago by ▴ 160
0
votes
0
replies
595
views
Comment: C: RNA-seq raw data lost
2.2 years ago by tianshenbio ▴ 160
3
votes
4
replies
595
views
RNA-seq raw data lost
RNA-Seq next-gen sequencing sequence
updated 2.2 years ago by 125k • written 2.2 years ago by ▴ 160
4
votes
2
replies
1.6k
views
What would be the best strategy to merge the GO annotation from blast2go, InterProScan, and eggnog
interproscan eggnog blast2go GO RNA-Seq
updated 2.4 years ago by 20 • written 2.6 years ago by ▴ 160
0
votes
0
replies
573
views
Comment: C: Can I directly blast mRNAs against nr database or I need to convert it to cds fi
2.5 years ago by tianshenbio ▴ 160
0
votes
0
replies
573
views
Comment: C: Can I directly blast mRNAs against nr database or I need to convert it to cds fi
2.5 years ago by tianshenbio ▴ 160
2
votes
4
replies
573
views
Can I directly blast mRNAs against nr database or I need to convert it to cds first
rna-seq annotation blast genome
updated 2.5 years ago by 13k • written 2.5 years ago by ▴ 160
3
votes
4
replies
2.3k
views
How to visualise KEGG pathway?
kegg enrichment RNA-Seq
updated 2.5 years ago by ▴ 430 • written 2.6 years ago by ▴ 160
0
votes
3
replies
1.9k
views
How do I remove certain sequences in fast based on header?
RNA-Seq sequence fasta
updated 2.6 years ago by ▴ 370 • written 2.6 years ago by ▴ 160
0
votes
0
replies
2.3k
views
Comment: C: How to visualise KEGG pathway?
2.6 years ago by tianshenbio ▴ 160
1
vote
2
replies
952
views
Blast: use best hit or top20 hits?
blast blast2go GO annotation RNA-Seq
2.6 years ago by tianshenbio ▴ 160
0
votes
0
replies
610
views
How to specify -s option in RNAhybrid to predict miRNA targets?
miRNA rna-seq sequence RNAhybrid
2.6 years ago by tianshenbio ▴ 160
0
votes
1
reply
881
views
Comment: C: Compare expression level of isoforms among conditions - TPM or deseq2 normalised
2.6 years ago by tianshenbio ▴ 160
0
votes
3
replies
881
views
Compare expression level of isoforms among conditions - TPM or deseq2 normalised counts?
RNA-Seq deseq2 tximport salmon
2.6 years ago by tianshenbio ▴ 160
0
votes
0
replies
1.8k
views
Comment: C: How to subset the output of tximport in DE analysis using DEseq2
2.6 years ago by tianshenbio ▴ 160
0
votes
0
replies
1.9k
views
Comment: C: Correct gtf file format (AGAT toolkit)
2.7 years ago by tianshenbio ▴ 160
0
votes
4
replies
1.9k
views
Correct gtf file format (AGAT toolkit)
RNA-Seq gff gtf AGAT gff3
updated 2.7 years ago by 7.5k • written 2.7 years ago by ▴ 160
0
votes
0
replies
1.9k
views
Comment: C: Correct gtf file format (AGAT toolkit)
2.7 years ago by tianshenbio ▴ 160
0
votes
0
replies
2.5k
views
Comment: C: Why sortmerna 4.2.0 runs so slow?
2.7 years ago by tianshenbio ▴ 160
0
votes
0
replies
2.3k
views
Comment: C: Is Stringtie a suitable tool for both gene and transcript quantification?
2.7 years ago by tianshenbio ▴ 160
0
votes
0
replies
2.3k
views
Comment: C: Is Stringtie a suitable tool for both gene and transcript quantification?
2.7 years ago by tianshenbio ▴ 160
10
votes
5
replies
2.3k
views
Is Stringtie a suitable tool for both gene and transcript quantification?
stringtie RNA-Seq sequencing featurecounts gene
updated 7 months ago by • 0 • written 2.7 years ago by ▴ 160
0
votes
1
reply
1.5k
views
Comment: C: Read counts at gene and transcript level - hisat2+Featurecounts or Stringtie?+de
2.7 years ago by tianshenbio ▴ 160
1
vote
3
replies
1.5k
views
Read counts at gene and transcript level - hisat2+Featurecounts or Stringtie?+deseq2
RNA-Seq featurecounts stringtie hisat2 deseq2
2.7 years ago by tianshenbio ▴ 160
0
votes
0
replies
437
views
Comment: C: Proper design in deseq2
2.7 years ago by tianshenbio ▴ 160
2
votes
2
replies
437
views
Proper design in deseq2
RNA-Seq deseq2 gene R
updated 2.7 years ago by 13k • written 2.7 years ago by ▴ 160
1
vote
1
reply
2.8k
views
Filter out DE genes with low counts in deseq2
RNA-Seq R gene deseq2
updated 2.7 years ago by 68k • written 2.7 years ago by ▴ 160
0
votes
0
replies
1.0k
views
Comment: C: Gffread_How to obtain fasta files of gene features (ORF, UTR) by transcripts bas
2.7 years ago by tianshenbio ▴ 160
0
votes
2
replies
1.0k
views
Gffread_How to obtain fasta files of gene features (ORF, UTR) by transcripts based on gff?
RNA-Seq Assembly genome sequence gene
2.7 years ago by tianshenbio ▴ 160
4
votes
3
replies
989
views
How to extract sequence of a specific feature in gff
rna-seq gff genome assembly
updated 2.7 years ago by 7.5k • written 2.7 years ago by ▴ 160
0
votes
0
replies
1.2k
views
Comment: C: After read mapping, count exons or genes?
2.7 years ago by tianshenbio ▴ 160
4
votes
2
replies
1.2k
views
After read mapping, count exons or genes?
RNA-Seq featurecounts genome
updated 2.7 years ago by 68k • written 2.7 years ago by ▴ 160
0
votes
1
reply
533
views
Comment: C: Many paralogous genes in GO enrichment results
2.7 years ago by tianshenbio ▴ 160
0
votes
1
reply
533
views
Comment: C: Many paralogous genes in GO enrichment results
2.7 years ago by tianshenbio ▴ 160
0
votes
5
replies
533
views
Many paralogous genes in GO enrichment results
RNA-Seq GO enrichment gene
2.7 years ago by tianshenbio ▴ 160
0
votes
2
replies
1.6k
views
How to generate a dataframe of deseq2 result excluding 'low counts' and 'outliers' ?
deseq2 RNA-Seq
updated 2.7 years ago by 84k • written 2.7 years ago by ▴ 160
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