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21 results • Page
1 of 1
Sort: Votes
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Votes
Replies
13
votes
20
replies
1.0k
views
Indexing a 2.6GB Plant Genome Using STAR (genomeGenerate Mode) Terminated Due to RAM Limit — Can the Genome Be Split for Indexing, or Are There Other…
star
RAN-seq
bam
genome
indexing
updated 3 hours ago by
Dunois
★ 2.8k • written 3 days ago by
Vijith
▴ 70
10
votes
12
replies
1.0k
views
dedup STAR transcriptome file using umi_tools
rna-seq
umi
transcriptome
umi_tools
8 hours ago by
Ar
★ 1.1k
9
votes
12
replies
931
views
Is a SLURM job array submission suitable for parallelizing multiple FASTQ file downloads?
parallel-computing
HPC
SLURM
FASTQ
updated 13 hours ago by
Dave Carlson
★ 1.9k • written 4 days ago by
kalavattam
▴ 280
4
votes
4
replies
1.6k
views
WGCNA kME vs kWithin Hub Gene Disparities
WGCNA
network
RNAseq
hub
module
updated 18 hours ago by
pkhuang
• 0 • written 10 months ago by
mhale
▴ 40
3
votes
5
replies
278
views
Create a fasta and gtf file
gtf
human
genome
fasta
2 hours ago by
Ar
★ 1.1k
2
votes
8
replies
808
views
SRAtoolkit --split-files output
sratoolkit
sra
fastq
3 hours ago by
tony_88888
• 0
2
votes
1
reply
1.6k
views
Is it better to phase two populations together or separately?
phasing
beagle
populations
updated 1 hour ago by
igor.alcaras
• 0 • written 9.0 years ago by
eyb
▴ 270
2
votes
4
replies
259
views
Contrasts for comparing condition within tissue
limma
deseq2
edgeR
contrasts
updated 11 hours ago by
SamGG
▴ 60 • written 1 day ago by
Ben
▴ 20
1
vote
2
replies
182
views
Regarding to Two-color microarray data analysis (DGE)
Differential-expression-analysis
updated 9 hours ago by
Ram
44k • written 1 day ago by
SHIRUI
▴ 10
1
vote
1
reply
156
views
QQ plot with small bump in the middle
QQ-plot.GWAS
updated 9 hours ago by
Ram
44k • written 1 day ago by
zhuzhu
▴ 10
0
votes
3
replies
564
views
Are there any preferred trajectory/pseudotime analyses that do not require an arbitrary start point?
scRNA-seq
pseudotime
monocle
scvelo
trajectory-analysis
22 hours ago by
nathansuek
• 0
0
votes
2
replies
93
views
No Cluster ID's present when making UMAP for Joint RNA and ATAC analysis: Signac 10x multiomic tutorial
multiomics
snATACseq
snRNAseq
10 minutes ago by
bgbs
• 0
0
votes
1
reply
97
views
ChIP-seq signal values
values
chipseq
signal
updated 19 hours ago by
1769mkc
★ 1.2k • written 20 hours ago by
SEJAL
• 0
0
votes
1
reply
144
views
News:
[Survey] Help Shape the Future of Genomic Data Storage Solutions!
genomics
bioinformatics
survey
solutions
storage
updated 16 hours ago by
Michael
55k • written 1 day ago by
spacecow
• 0
0
votes
0
replies
61
views
Extract directionality from Reactome
pathways
Reactome
biopax
16 hours ago by
Nikolas
• 0
0
votes
4
replies
260
views
NGS data analysis
NGS
eukaryotic
updated 12 hours ago by
colindaven
6.9k • written 1 day ago by
Acervo
• 0
0
votes
0
replies
60
views
database of gmap file for GLIMPSE2 imputation
gmap
GLIMPSE2
12 hours ago by
cautree
• 0
0
votes
0
replies
40
views
m6A modification prediction using Nanopore DRS data (RNA004)
m6A
DRS
Nanopore
updated 4 hours ago by
GenoMax
147k • written 6 hours ago by
baibhu1234
• 0
0
votes
0
replies
52
views
How to interpret ANCOM-BC results when diff is true but passed_ss is false?
differential-abundance
updated 4 hours ago by
Ram
44k • written 7 hours ago by
benkosta
• 0
0
votes
3
replies
46
views
Clarification with vcf file
quality
VCF
vcf
score
1 hour ago by
Nesma
• 0
0
votes
0
replies
13
views
Phasing alleles with SHAPEIT4: cases and controls together or separately?
cases-controls
separately
together
case-control
phasing
1 hour ago by
igor.alcaras
• 0
21 results • Page
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Recent Votes
Comment: Indexing a 2.6GB Plant Genome Using STAR (genomeGenerate Mode) Terminated Due to
Comment: Difference in relative abundance between MicrobiomeDB and r phyloseq
Comment: Discrepancy Between Genus and Phylum Level Results in ANCOMBC2
Answer: Create a fasta and gtf file
A: Adding column annotation to heatmap using Complexheatmap
Avoiding the Pitfalls of the Anaconda License: A Practical Guide
Comment: Indexing a 2.6GB Plant Genome Using STAR (genomeGenerate Mode) Terminated Due to
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Recent Replies
Answer: No Cluster ID's present when making UMAP for Joint RNA and ATAC analysis: Signac
by
bgbs
• 0
Thanks for your response. I was able to fix this by first making the UMAP for the snRNAseq assay. I re-ran FindNeighbors and then re-ran Fi…
Comment: Clarification with vcf file
by
Nesma
• 0
I didn't know I'm sorry, I think I fixed it
Answer: Is it better to phase two populations together or separately?
by
igor.alcaras
• 0
Here am I, found your post 9 years later, and I'm with the same question... did you find it out?
Comment: Clarification with vcf file
by
Pierre Lindenbaum
164k
> let me know if you see anything strange? the depth DP at all your sites is only '1'
Comment: Clarification with vcf file
by
Pierre Lindenbaum
164k
Please do not paste screenshots of plain text content, it is counterproductive. You can copy paste the content directly here (using the cod…
Comment: Create a fasta and gtf file
by
Ar
★ 1.1k
@juke34 I have another question. Is there a way to extract the longest gene sequence without the introns? For example: `agat_sp_extract_se…
Answer: SRAtoolkit --split-files output
by
tony_88888
• 0
I did not do anything to the files. I corrected this problem by using -F with fastq dump. When I took a look at line 4 of the input files t…
Comment: Indexing a 2.6GB Plant Genome Using STAR (genomeGenerate Mode) Terminated Due to
by
Dunois
★ 2.8k
Sure, but why? What's the end goal of producing this genome? Is it simply having the genome published or is there some other set of downstr…
Answer: No Cluster ID's present when making UMAP for Joint RNA and ATAC analysis: Signac
by
jared.andrews07
★ 18k
Your object has no clusters set as `Idents`. So yes, you need to call clusters first. ``` liftoff_1_MI5_V1_SO <- FindNeighbors(liftoff_1_…
Comment: Indexing a 2.6GB Plant Genome Using STAR (genomeGenerate Mode) Terminated Due to
by
colindaven
6.9k
I think Genomax may well be right, there is something fishy about your stats. Please use conda to install bbmap (from memory) conda i…
Comment: Create a fasta and gtf file
by
Ar
★ 1.1k
Thank you! This is very helpful.
Comment: Create a fasta and gtf file
by
Ar
★ 1.1k
This is awesome. Thank you and it worked like a charm!
Comment: dedup STAR transcriptome file using umi_tools
by
Ar
★ 1.1k
Thank you, I didn't know about the green check mark. I did it now, thanks!
Comment: Regarding to Two-color microarray data analysis (DGE)
by
Ram
44k
Please do not use `bioinformatics` as a tag unless your post is about the field of bioinformatics itself. For proper examples, please see F…
Comment: Indexing a 2.6GB Plant Genome Using STAR (genomeGenerate Mode) Terminated Due to
by
Vijith
▴ 70
@dunois What I have is an assembled genome of a monocot plant. The assembly was performed using clc genomics. Prior to assembly, I had esti…
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