2
votes
2
replies
141
views
1
vote
3
replies
236
views
1
vote
3
replies
331
views
1
vote
6
replies
2.7k
views
updated 12 minutes ago by
dimpleadiwal050896
•
0
•
written 4.9 years ago by
ggman
▴
90
1
vote
6
replies
314
views
3 hours ago by
marco.barr
▴
80
0
votes
0
replies
36
views
0
votes
0
replies
37
views
0
votes
1
reply
133
views
updated 3 hours ago by
christopher medway
▴
450
•
written 12 hours ago by
Tigran
•
0
0
votes
1
reply
118
views
0
votes
2
replies
59
views
0
votes
0
replies
13
views
0
votes
1
reply
50
views
0
votes
0
replies
42
views
updated 20 minutes ago by
GenoMax
141k
•
written 7 hours ago by
aliraza3119
•
0
0
votes
1
reply
47
views
0
votes
9
replies
2.5k
views
6 follow
Recent Votes
Recent Locations •
All
Singapore, 1 minute ago
Turkey, 2 minutes ago
Belgium, 2 minutes ago
Russia, 3 minutes ago
UK, 4 minutes ago
United Kingdom, 4 minutes ago
United Kingdom, 5 minutes ago
Recent Awards •
All
Popular Question to
SaltedPork
▴
170
Popular Question to
christopher medway
▴
450
Popular Question to
Mohita
▴
50
Popular Question to
Zhenyu Zhang
★
1.2k
Popular Question to
WUSCHEL
▴
750
Teacher to
Mensur Dlakic
★
27k
Teacher to
Jeremy Leipzig
22k
Recent Replies
Comment: Segmentation fault using gemma
by
dimpleadiwal050896
•
0
Hello, were you able to rectify this. If yes, do tell. I am getting a similar error. Thank you
Comment: Can I merge Hi-C fastq files from different lanes?
by
GenoMax
141k
If the data is a technical sequencing replicate i.e. the same pool of samples has run on two lanes then you can indeed merge the two lanes …
Comment: What should I consider as FASTA for dataset?
by
GenoMax
141k
> Should I use the shortened FASTA from pdb or should I use the full FASTA for my dataset?
What analysis are you trying to use the data fo…
Comment: Is it possible to bulk download files from GEO repository?
by
GenoMax
141k
Galaxy specific requests are best posted on their help forum: https://help.galaxyproject.org/
Answer: Two references 1. genome 2. plasmid for bowtie2
by
GenoMax
141k
You can simply `cat` all reference sequences together. Create an index file with `bowtie2` and then align as usual.
Answer: High Malat-1 expression in single cell data
by
ATpoint
82k
My comment is general since I've never looked at this gene specifically, but metrics of poor cell quality in my experience never come alone…
Comment: What does it mean single base resolution in sequencing?
by
GenoMax
141k
>You known, 90% + genomic region is transcripted. So, total RNA-seq theoretically covers most genome region.
Perhaps but not all of that …
Answer: Using Delly/Pindel/breakdancer for Identifying Transgene Insertion Sites in Mous
by
trausch
★
1.9k
For [delly][1], we usually augment the mouse reference genome with the additional sequence, then remap and then look in the delly output fo…
Comment: NGS forensics: how to know if data is fabricated
by
i.sudbery
19k
Thats very intersting! What are the features in your classifier?
Answer: Limma Analysis Agilent Microarray Data (GPL1708)
by
hagl
▴
10
Thank you very much for the response. Following your recommendation in pursuing option one by selecting highest expressions and subsequent …
Answer: RNAseq one control two conditions, shared and exclusive genes
by
Mohamed Abderrahmane
▴
20
I think it would be pertinent to use DESEq2 to perform two comparisons: the first one between the control group and condition 1, and the se…
Comment: Longitudinal analysis of subpopulations: which approach is better?
by
Lluís R.
★
1.2k
Many thanks for the helpful comment. Indeed, I read some of them, but I was not fully convinced and I missed the link FAQ of DESeq2. The re…
Comment: Longitudinal analysis of subpopulations: which approach is better?
by
ATpoint
82k
Keeping data together is most powerful and most convenient as you have a single analysis object and a single count matrix. I would always d…
Comment: Questions about a bug when transferring cram file to bam file
by
jkbonfield
★
1.2k
Assuming you have network access and the md5sum is registered with the EBI's reference server, yes - it'll be downloaded and cached locally.
Comment: Downsampling fastq file
by
marco.barr
▴
80
I followed your advice and it seems that I'm getting results comparable to what I was getting before. Upon checking with `wc -l` on the ori…
Traffic: 2611 users visited in the last hour
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by the
version 2.3.6
version 2.3.6