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300 results • Page
2 of 6
Sort: Rank
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Votes
Replies
4
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3
replies
727
views
Forum:
When to ask for rerun from vendors
ngs-vendor
updated 4 days ago by
Ram
39k • written 3.7 years ago by
microfuge
★ 1.9k
4
votes
12
replies
1.2k
views
Forum:
publicly available data
arrayexpress
GEO
SRA
updated 1 day ago by
Ram
39k • written 3.7 years ago by
Konstantinos Yeles
▴ 110
3
votes
8
replies
2.7k
views
6 follow
Forum:
SSD or HHD for genome analysis
hardware
genome
sequencing
updated 1 day ago by
Ram
39k • written 3.7 years ago by
maksjytov.nail
▴ 10
2
votes
7
replies
897
views
Forum:
Future prospects: NGS vs Gene editing (using CRISPR CAS9, AAVs and similar methods)
NGS
CRISPR-Cas9
updated 1 day ago by
Ram
39k • written 3.7 years ago by
Gabriel
▴ 120
1
vote
0
replies
676
views
Forum:
What are the 10 most exciting developments on the horizon in precision medicine?
precision-medicine
updated 1 day ago by
Ram
39k • written 3.7 years ago by
Vincent Laufer
★ 2.9k
3
votes
2
replies
5.7k
views
Forum:
Best book for learning Next-Generation Sequencing Data Analysis?
ngs
updated 18 days ago by
Ram
39k • written 5.3 years ago by
Abdul Rafay Khan
★ 1.2k
21
votes
15
replies
1.5k
views
9 follow
Forum:
Working abroad without a PhD
career
updated 1 day ago by
Ram
39k • written 3.7 years ago by
Rox
★ 1.4k
13
votes
4
replies
984
views
Forum:
The shortcoming of shiny app in building webserver
R
shiny
webserver
updated 4 days ago by
Ram
39k • written 3.7 years ago by
harrypotterandsbt
▴ 110
1
vote
3
replies
722
views
Forum:
The pStacks module of Stacks has been removed?
software-error
updated 4 days ago by
Ram
39k • written 3.8 years ago by
nbarthelemy01
▴ 20
3
votes
3
replies
905
views
Forum:
Large Multi-omics Project -- Dev Approach
snp
RNA-Seq
sequencing
updated 4 days ago by
Ram
39k • written 3.8 years ago by
CK
▴ 10
4
votes
5
replies
1.2k
views
7 follow
Forum:
Career related query
MSc
career
updated 11 days ago by
Ram
39k • written 4.5 years ago by
ravi.eshwari
▴ 10
12
votes
4
replies
1.3k
views
Forum:
Bioinformatics and biotechnology market in future.
genome
next-gen
updated 4 days ago by
Ram
39k • written 3.8 years ago by
mona11224
• 0
3
votes
0
replies
879
views
Forum:
Good books on application of Machine Learning in Bioinformatics
book
machine-learning
updated 4 days ago by
Ram
39k • written 3.8 years ago by
The
▴ 180
0
votes
2
replies
1.1k
views
Forum:
What should I choose between a thesis and a project for masters in bioinformatics?
thesis
project
updated 4 days ago by
Ram
39k • written 3.8 years ago by
koushikayaluri
▴ 70
115
votes
35
replies
38k
views
20 follow
Forum:
Snakemake vs. Nextflow: strengths and weaknesses
snakemake
nextflow
updated 25 days ago by
Ram
39k • written 6.0 years ago by
ropolocan
▴ 760
4
votes
0
replies
915
views
Forum:
Plotting Ensembl gene name changes between versions
R
ensembl
updated 4 days ago by
Ram
39k • written 3.8 years ago by
Corentin
▴ 550
37
votes
21
replies
10k
views
10 follow
Forum:
Orientation of PE reads a review of --fr --ff and --rf meanings
RNA-Seq
updated 12 days ago by
Ram
39k • written 4.6 years ago by
Juke34
7.9k
1
vote
2
replies
804
views
Forum:
What Are The Big Problems/Current Trends In Proteomics?
proteomics
sequencing
updated 5 days ago by
Ram
39k • written 3.9 years ago by
MtoZ-Biolabs
• 0
3
votes
4
replies
2.8k
views
6 follow
Forum:
Links for forums or discussion groups about machine learning, aging, genomics and artificial intelligence?
genome
updated 18 days ago by
Ram
39k • written 5.3 years ago by
tfhahn
▴ 50
0
votes
4
replies
1.7k
views
Forum:
Multiple Sequence Alignment Applications
snp
updated 5 days ago by
Ram
39k • written 3.9 years ago by
manimphil89
• 0
4
votes
13
replies
1.3k
views
Forum:
Looking for Participants: Meenta NovaSeq RunShare Beta
RunShare
next-gen
NovaSeq
updated 5 days ago by
Ram
39k • written 3.9 years ago by
Torrey Ah-Tye
▴ 10
2
votes
5
replies
1.4k
views
Forum:
Looking for co-author with Bioinformatics background for paper using Machine Learning in Biology
neural-networks
lncRNA
RNN
FEELnc
updated 13 days ago by
Ram
39k • written 4.7 years ago by
sieva1
▴ 20
14
votes
10
replies
1.6k
views
Forum:
WGBS alignment with BitMapperBS
BitMapperBS
bismark
updated 5 days ago by
Ram
39k • written 3.9 years ago by
Shicheng Guo
★ 9.3k
13
votes
6
replies
940
views
Forum:
To Cloud or Not to Cloud - Privacy implications of genetic data sharing
security
diagnostics
DNA-Seq
updated 7 days ago by
Ram
39k • written 3.9 years ago by
David Langenberger
9.9k
0
votes
1
reply
745
views
Forum:
Tools and data for plant proteins
alignment
sequencing
updated 6 days ago by
Ram
39k • written 3.9 years ago by
Shide
• 0
14
votes
5
replies
6.1k
views
Forum:
Seurat vs Monocle
R
RNA-Seq
next-gen
single-cell
updated 5 days ago by
Ram
39k • written 3.9 years ago by
Arup Ghosh
3.2k
5
votes
3
replies
712
views
Forum:
price of MSI detection by NGS for 1 person
next-gen
updated 6 days ago by
Ram
39k • written 3.9 years ago by
jandoubi.nouha333
▴ 20
3
votes
2
replies
604
views
Forum:
Application suggestions for slackbot
slack
updated 6 days ago by
Ram
39k • written 4.0 years ago by
Eric Lim
★ 2.1k
11
votes
13
replies
3.5k
views
6 follow
Forum:
NGS Data storage solutions for small organisations or big labs
big-data
HTS
storage
NGS
metagenomics
updated 6 days ago by
Ram
39k • written 4.0 years ago by
bioinfo
▴ 830
1
vote
5
replies
1.2k
views
Forum:
PhD: Research interest or better program?
PhD
updated 6 days ago by
Ram
39k • written 4.0 years ago by
Sslero
• 0
11
votes
5
replies
4.2k
views
Forum:
Bioinformatics Workstation Suggestions
RNA-Seq
next-gen
workstation
updated 6 days ago by
Ram
39k • written 4.0 years ago by
akh22
▴ 90
12
votes
13
replies
1.8k
views
6 follow
Forum:
Recovering bam files after unknown deletion in the storage
photorec
bam
sequencing
updated 6 days ago by
Ram
39k • written 4.0 years ago by
Gabriel Wajnberg
▴ 70
1
vote
1
reply
941
views
Forum:
Companies and salary for a PhD in Drug Design in Asia
salary
drug-design
updated 6 days ago by
Ram
39k • written 4.0 years ago by
pierrellompart
• 0
1
vote
1
reply
1.5k
views
Forum:
Free Bioinformatics Training Resources (for non-bioinformaticians)
RNA-Seq
updated 6 days ago by
Ram
39k • written 4.0 years ago by
elia.brodsky
▴ 340
4
votes
4
replies
1.4k
views
Forum:
When will the hg19/grch37 finally become obsolete?
Reference-Genome
updated 6 days ago by
Ram
39k • written 4.0 years ago by
gdaly9000
▴ 10
3
votes
1
reply
826
views
Forum:
Recommend papers: automated vs manual downstream analysis
downstream-analysis
reproducibility
updated 6 days ago by
Ram
39k • written 4.1 years ago by
c.clarido
▴ 110
0
votes
0
replies
615
views
Forum:
Survey for job context
survey
interview
updated 6 days ago by
Ram
39k • written 4.0 years ago by
campeonapm
• 0
4
votes
0
replies
3.3k
views
Forum:
How can unique molecular identifiers (UMIs) help to reduce quantitative biases?
NGS
Variant-Calling
Unique-Molecular-Identifier
updated 6 days ago by
Ram
39k • written 4.0 years ago by
David Langenberger
9.9k
11
votes
5
replies
1.2k
views
Forum:
What bioinformatic training?
training
RNA-Seq
illumina
updated 6 days ago by
Ram
39k • written 4.0 years ago by
Peter Horbatz
▴ 150
5
votes
5
replies
2.0k
views
Forum:
BCL files as deliverables from a sequencing center
core-facility
service-provider
updated 6 days ago by
Ram
39k • written 4.0 years ago by
Ido Tamir
5.2k
4
votes
4
replies
990
views
Forum:
What skills are required for an informatics scientist?
informatics
scientist
updated 6 days ago by
Ram
39k • written 4.0 years ago by
Shicheng Guo
★ 9.3k
0
votes
1
reply
806
views
Forum:
An idea for a project in ML
ML
matlab
machine-learning
data-science
updated 6 days ago by
Ram
39k • written 4.1 years ago by
eheilbru
• 0
10
votes
4
replies
5.4k
views
Forum:
Bioinformatics: Which language should I learn
programming
updated 7 days ago by
Ram
39k • written 4.1 years ago by
MEHAR ALI RAZA
• 0
3
votes
7
replies
766
views
Forum:
Construct a small computing server for GWAS Analysis
assembly
updated 7 days ago by
Ram
39k • written 4.1 years ago by
mkh
▴ 60
1
vote
1
reply
538
views
Forum:
Whither my badges?
meta
updated 7 days ago by
Ram
39k • written 4.1 years ago by
cdsouthan
★ 1.9k
2
votes
3
replies
664
views
Forum:
What are the major types of choices that affect an analysis?
reproducibility
updated 7 days ago by
Ram
39k • written 4.1 years ago by
Jeremy Leipzig
21k
5
votes
2
replies
1.0k
views
Forum:
ISMB bioinfo-core workshop call for lightning talks
conferences
updated 7 days ago by
Ram
39k • written 4.1 years ago by
Madelaine Gogol
5.3k
7
votes
9
replies
1.2k
views
Forum:
10 Most important topic in Human evolutionary genomics
evolutionary-genomics
evolutionary-medicine
updated 7 days ago by
Ram
39k • written 4.2 years ago by
aloke205
▴ 40
113
votes
53
replies
5.3k
views
16 follow
Forum:
Middle author, but not first author projects. How would you approach this?
authorship
career-development
updated 7 days ago by
Ram
39k • written 4.2 years ago by
pet.bioinformatician
▴ 170
1
vote
3
replies
826
views
Forum:
Poorly designed "omics" experiment
RNA-Seq
next-gen
ChIP-Seq
updated 7 days ago by
Ram
39k • written 4.2 years ago by
Baby Bioinformatician
▴ 100
300 results • Page
2 of 6
Recent Votes
A: Extract Reads From A Bam File That Fall Within A Given Region
Comment: Masking before RNA-Seq Alignment and Gene Prediction in Plant Genomes
Comment: Alignment of case vs. control from different origin
A: understanding bedtools coverage output
Answer: Alignment of case vs. control from different origin
Comment: Alignment of case vs. control from different origin
Comment: Gene prediction software
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Recent Replies
Comment: Differential protein expression analysis
by
Ribo
▴ 40
Thank you! Is there a recommended tutorial for proteomics analysis?
Comment: Limma returned only positive logFC values
by
melissachua90
▴ 40
I clustered the samples into subtypes and am now comparing the differential expression across the subtypes.
Answer: Limma returned only positive logFC values
by
Gordon Smyth
★ 6.1k
No, your code isn't correct. You are testing an intercept equal to zero instead of testing for a logFC. I wonder what comparison you were t…
Answer: Extract sequences from a fastq file by a list of IDs
by
GenoMax
129k
Using `filterbyname.sh` from [**BBMap suite**][1]: You need to include the `/1` in the header in your list file (here I am using the `nam…
Comment: Limma returned only positive logFC values
by
melissachua90
▴ 40
Yeah, I added the design matrix. `design <- model.matrix(~0+group)`. These are raw counts.
Comment: Masking before RNA-Seq Alignment and Gene Prediction in Plant Genomes
by
Ayish
• 0
Thank you for reply. I have Illumina paired-end reads. Would it be fine if I use hard-masking for STAR and soft-masked genome for BRAKER2? …
Comment: Masking before RNA-Seq Alignment and Gene Prediction in Plant Genomes
by
Darked89
4.4k
Soft masking the genome (unless something changed) not make any difference for STAR. Depending on how your RNA-Seq is done (Illumina? paire…
Answer: How to handle NaN in emmax Kinship matrix?
by
Thu
• 0
Hi! Thanks for sharing your solution! It works! :)
Comment: Gene prediction software
by
Darked89
4.4k
Looks like there are five Clarias genomes: * https://www.ncbi.nlm.nih.gov/genome/?term=txid13012[Organism:exp] The most complete seems t…
Comment: Alignment of case vs. control from different origin
by
sativus
▴ 10
Again, thank you so much for these very clearly explained summaries. After reading some articles on the matter, i feel i have a much better…
Answer: Extract sequences from a fastq file by a list of IDs
by
colindaven
4.9k
Some people I know have used filter-fastq successfully: https://github.com/Floor-Lab/filter-fastq
Comment: Extract sequences from a fastq file by a list of IDs
by
Pierre Lindenbaum
154k
> but none of them work for me https://meta.stackexchange.com/questions/147616/
Comment: Extract sequences from a fastq file by a list of IDs
by
Pierre Lindenbaum
154k
https://meta.stackexchange.com/questions/147616/
Comment: Extract sequences from a fastq file by a list of IDs
by
mhpakdel96
• 0
I have tried this one before but the output file is empty
Comment: Extract sequences from a fastq file by a list of IDs
by
mhpakdel96
• 0
I have tried all of them but none of them work for me
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