0
votes
0
replies
19
views
0
votes
0
replies
30
views
0
votes
0
replies
71
views
8 hours ago by
HarperReed
•
0
1
vote
0
replies
52
views
0
votes
0
replies
50
views
11 hours ago by
SineWave
•
0
0
votes
0
replies
51
views
0
votes
0
replies
48
views
13 hours ago by
turcoa1
•
0
0
votes
0
replies
65
views
0
votes
0
replies
76
views
0
votes
1
reply
112
views
updated 21 hours ago by
ATpoint
82k
•
written 22 hours ago by
AaronJaime
•
0
0
votes
2
replies
167
views
0
votes
2
replies
205
views
0
votes
2
replies
217
views
0
votes
11
replies
474
views
0
votes
7
replies
253
views
Recent Votes
Recent Locations •
All
Dublin, Ireland, just now
San Francisco, just now
United States, 2 minutes ago
Spain, 2 minutes ago
India, 5 minutes ago
Seattle, WA USA, 5 minutes ago
United States, 5 minutes ago
Recent Awards •
All
Popular Question to
rheab1230
▴
140
Popular Question to
BioinfGuru
★
1.7k
Popular Question to
vinayjrao
▴
250
Supporter to
Jalil Sharif
▴
80
Popular Question to
sil_bioinfo
▴
40
Recent Replies
Comment: how to combine multiple RNAseq count files into a single dataframe in R and unix
by
BioinfGuru
★
1.7k
Hi,
Just thought I'd share this code snippet here for when each file contains multiple samples. I tried using lapply (as suggested by Ram)…
Comment: Low mapping rate with Salmon
by
i.sudbery
19k
Take your STAR alignment and sum all the counts for each gene. You can either do this by providing an annotation to STAR, or by running fea…
Comment: How to convert plink files to Hapmap Format
by
bk11
★
2.4k
If you run your data with plink 2.0, you will have ERRCODE column in your result file showing the reason behind "NA" p-value.
https://www.b…
Comment: genome assembly records not present in assembly_summary.txt
by
sapuizait
▴
10
jesus its in the Genbank file and I was looking at the refseq! I m such a moron - thanks for pointing it out -sorry about that :(
Comment: ScRNA data question
by
bk11
★
2.4k
I wonder how the `Vlnplots` will look if you normalize the data using `NormalizeData()` function in `Seurat`. The flat line in your plot ar…
Comment: Low mapping rate with Salmon
by
GenoMax
141k
> Does that mean that the reads that are not mapped to my trascriptome are not exons/coding genes?
Reads are likely aligning in regions wh…
Comment: How Can We Find The Info For 3'Utr And 5'Utr In Gencode Gtf File?
by
cmdcolin
★
3.8k
the gencode link is broken now but here is a back up of that blogpost on archive https://web.archive.org/web/20130618221342/http://gencodeg…
Comment: Low mapping rate with Salmon
by
Patadu94
•
0
I'll try to make the decoy file again but I remember you also told me that it is only recommended and not mandatory for running `salmon`. I…
Comment: Low mapping rate with Salmon
by
Patadu94
•
0
For doing this, should I just look at the log.out.file?
Answer: Heatmap and rna-seq
by
pinheirofabiano
▴
10
install.packages("pheatmap")
library(pheatmap)
setwd("/Users/data_analysis/results")
data <- read.table(file…
Comment: Low mapping rate with Salmon
by
Patadu94
•
0
Thanks for the link GenoMax. But I was wondering, should not alignment and mapping have a similar rate and thus be correlated? In my case I…
Answer: Create a new bed file with all pairwise combinations between two other bed files
by
Pierre Lindenbaum
161k
bedtools intersect \
-a <(sort -t $'\t' -k1,1 -k2,2n A.bed) \
-b <(awk '{X=250000;P=int($2);printf("%s\t%d\…
Comment: Create a new bed file with all pairwise combinations between two other bed files
by
J
•
0
The command lines I wrote out for you are the ones I actually used. My original reference to bedtools intersect was because I assumed the b…
Comment: ScRNA data question
by
starswillfade
▴
10
I just thought it was weird that considering each dot is a cell their expressions were all on a single line instead of being spread out lik…
Comment: ScRNA data question
by
starswillfade
▴
10
![enter image description here][1] I am already using Seurat V5 I think. I am using SCTransform as it was recommended by the seurat page. I…
Traffic: 3861 users visited in the last hour
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by the
version 2.3.6
version 2.3.6