0
votes
0
replies
99
views
0
votes
1
reply
155
views
0
votes
0
replies
78
views
0
votes
3
replies
613
views
0
votes
3
replies
590
views
updated 1 day ago by
Ram
41k
•
written 13 months ago by
mresmaeili995
•
0
0
votes
2
replies
656
views
0
votes
1
reply
689
views
updated 1 day ago by
Ram
41k
•
written 3.6 years ago by
aiswaryabioinfo
▴
30
0
votes
7
replies
4.2k
views
updated 1 day ago by
Ram
41k
•
written 5.5 years ago by
nasreenbano91
•
0
0
votes
1
reply
135
views
0
votes
3
replies
199
views
0
votes
4
replies
264
views
0
votes
2
replies
185
views
0
votes
1
reply
132
views
0
votes
4
replies
657
views
0
votes
0
replies
70
views
0
votes
0
replies
63
views
19 hours ago by
junhuili
•
0
0
votes
1
reply
130
views
0
votes
1
reply
98
views
0
votes
1
reply
131
views
0
votes
2
replies
126
views
updated 7 hours ago by
Istvan Albert
99k
•
written 10 hours ago by
Manuel
•
0
0
votes
0
replies
688
views
0
votes
0
replies
97
views
6 days ago by
maxlaubstein
•
0
0
votes
0
replies
103
views
6 days ago by
hellokwmin
•
0
0
votes
1
reply
169
views
updated 6 days ago by
Nitin Narwade
★
1.5k
•
written 7 days ago by
tss243
•
0
0
votes
2
replies
308
views
updated 6 days ago by
fracarb8
★
1.3k
•
written 7 days ago by
synat.keam
▴
80
0
votes
1
reply
156
views
0
votes
3
replies
261
views
updated 6 days ago by
Istvan Albert
99k
•
written 6 days ago by
Jingfang
•
0
0
votes
0
replies
131
views
0
votes
0
replies
130
views
0
votes
1
reply
450
views
0
votes
0
replies
146
views
0
votes
1
reply
175
views
0
votes
0
replies
146
views
0
votes
4
replies
1.2k
views
updated 5 days ago by
ben@f
▴
10
•
written 11 months ago by
javanokendo
▴
20
0
votes
1
reply
196
views
0
votes
0
replies
158
views
0
votes
1
reply
206
views
updated 5 days ago by
Istvan Albert
99k
•
written 5 days ago by
meera
•
0
0
votes
0
replies
131
views
Recent Votes
Recent Locations •
All
China, 1 minute ago
Chile, 1 minute ago
United States, 5 minutes ago
United States, 7 minutes ago
Republic of Ireland, 8 minutes ago
Seattle, WA USA, 8 minutes ago
Memphis, TN, 8 minutes ago
Recent Awards •
All
Popular Question to
samreen.jasvi
•
0
Scholar to
jared.andrews07
★
16k
Popular Question to
Mr Locuace
▴
150
Popular Question to
benjamin.pyenson
•
0
Popular Question to
Malachi Griffith
19k
Popular Question to
awhale01
•
0
Recent Replies
Comment: Resolving over clustered NGS with Q-scores
by
sam.himes92
•
0
The system that we used was [Aviti][1]. Our sequencing core was the one who quantified the loading/library concentration. Sorry I don't hav…
Comment: Where can I get a list of SNPs mapping overlapping genes in humans?
by
Alex Reynolds
35k
I modified the answer with an approach for that use case.
Comment: Where can I get a list of SNPs mapping overlapping genes in humans?
by
Ram
41k
OP is looking for overlapping genes - genes with presumably different gene IDs that share some loci. I think the `genes.bed` creation logic…
Comment: Where can I get a list of SNPs mapping overlapping genes in humans?
by
Alex Reynolds
35k
Note: The file `snps.bed` will be very large. You'll need sufficient disk space for this step.
Answer: Where can I get a list of SNPs mapping overlapping genes in humans?
by
Alex Reynolds
35k
Given files `genes.bed` and `snps.bed`, you could do something like:
```
$ bedmap --echo --echo-map-id --delim '\t' genes.bed snps.bed > a…
Answer: How to add exon annotations to genome annotation
by
alex.zaccaron
▴
380
You can use [AGAT][1] to process your annotation file to add the missing exons. I believe `agat_convert_sp_gxf2gxf.pl -g no_exons.gff -o wi…
Comment: Annotation Visualization IGV
by
Pierre Lindenbaum
158k
and you got answer that should be validate (green tick), or people asked you questions but got no answers.
Comment: Annotation Visualization IGV
by
Pierre Lindenbaum
158k
you asked questions before: review, comment and/or validate them please: https://www.biostars.org/p/9530777/ ; https://www.biostars.org/p/9…
Comment: Ambient RNA removal method that generates whole (integer) counts
by
EK
•
0
Thanks, Jared. Sounds like rounding counts is the simplest approach for now. :)
Comment: Resolving over clustered NGS with Q-scores
by
Trivas
★
1.5k
Could you tell us which sequencer you used, how you quantified your library size and concentration, and what your loading concentration was…
Comment: Resolving over clustered NGS with Q-scores
by
sam.himes92
•
0
I should have mentioned, we ran both Read 1 and 2 well past the points of interest. For each read we ran 75 cycles.
Answer: Resolving over clustered NGS with Q-scores
by
Trivas
★
1.5k
It might be because Illumina recommends 26 cycles for Read 1: https://knowledge.illumina.com/instrumentation/general/instrumentation-genera…
Comment: Resolving over clustered NGS with Q-scores
by
ATpoint
78k
Has phiX been spiked in to increase nucleotide diversity.
Traffic: 1365 users visited in the last hour
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by the
version 2.3.6
version 2.3.6