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0
votes
1
reply
572
views
Best set of tools / workflows for predicting the biosynthesis pathways of complex organic molecules
ngs
pathways
bioinformatics
updated 9 hours ago by
Joe
22k • written 10 hours ago by
Mark
▴ 60
1
vote
3
replies
6.1k
views
Best way to do protein structure guided similarity search?
alphafold
BLAST
structure
proteins
updated 5 days ago by
Dunois
★ 2.9k • written 6 days ago by
Mark
▴ 60
2
votes
2
replies
9.6k
views
Automatically annotating a feature using a genbank file
Annotation
genbank
wgs
updated 10 days ago by
Pierre Lindenbaum
166k • written 10 days ago by
Mark
▴ 60
4
votes
2
replies
1.1k
views
combine hifiasm and hifiasm-ont assemblies.
wgs
ngs
genomics
sequencing
updated 6 weeks ago by
shelkmike
★ 1.8k • written 6 weeks ago by
Mark
▴ 60
0
votes
1
reply
354
views
Run hifiasm without error correction module?
hifiasm
assembly
wgs
nanopore
genomics
updated 12 weeks ago by
shelkmike
★ 1.8k • written 12 weeks ago by
Mark
▴ 60
1
vote
0
replies
450
views
Any tips for optimizing an all vs all alignment with minimap2
mapping
nanopore
genomics
minimap2
3 months ago by
Mark
▴ 60
0
votes
0
replies
523
views
Comment:
Comment: How to fix this this tangle in my bacterial genome assembly
3 months ago by
Mark
▴ 60
2
votes
2
replies
523
views
How to fix this this tangle in my bacterial genome assembly
misassembly
assembly
genomics
ngs
3 months ago by
Mark
▴ 60
0
votes
2
replies
602
views
Is there a way to import a large amount of regions of interest into IGV
sequencing
igv
genomics
ngs
updated 6 months ago by
GenoMax
153k • written 6 months ago by
Mark
▴ 60
2
votes
1
reply
998
views
Recommendations for Reference-guided de novo assembly assembly approaches or pipelines?
ont
assembly
wgs
nanopore
genomics
updated 6 months ago by
shelkmike
★ 1.8k • written 6 months ago by
Mark
▴ 60
3
votes
1
reply
987
views
Subset reads based on Phred-score ranges
phred
sequencing
ngs
updated 6 months ago by
GenoMax
153k • written 6 months ago by
Mark
▴ 60
3
votes
7
replies
1.1k
views
Best way to assess relatedness of eukaryotic organisms from sequencing reads?
phylogenomics
phylogenetics
ngs
kmer
sequencing
updated 6 months ago by
colindaven
7.9k • written 6 months ago by
Mark
▴ 60
0
votes
0
replies
463
views
DeChat Nanopore read correction with Medaka correction?
ont
medaka
wgs
dechat
nanopore
7 months ago by
Mark
▴ 60
0
votes
0
replies
650
views
Looking to Simulate Nanopore R10.4.1 and PacBio HiFi whole genome sequencing reads for 3 specific organisms
pacbio
nanopore
sequencing
reads
wgs
7 months ago by
Mark
▴ 60
6
votes
3
replies
1.0k
views
Convert Long Nanopore reads to Illumina Paired end reads
illumina
sequencing
reads
nanopore
updated 7 months ago by
Pierre Lindenbaum
166k • written 7 months ago by
Mark
▴ 60
2
votes
1
reply
817
views
Question about Kraken2 core_nt vs nt database results.
database
kraken2
kmer
ncbi
updated 7 months ago by
GenoMax
153k • written 7 months ago by
Mark
▴ 60
0
votes
1
reply
690
views
Comment:
Comment: Kmerfinder database with bacteria, archea, and eukaryotic data
8 months ago by
Mark
▴ 60
0
votes
3
replies
690
views
Kmerfinder database with bacteria, archea, and eukaryotic data
kmer
updated 8 months ago by
lieven.sterck
15k • written 8 months ago by
Mark
▴ 60
0
votes
2
replies
646
views
Question about metabolic pathway prediction using only DNA sequencing data
metabolism
genomics
ngs
updated 9 months ago by
hakimbazol
▴ 60 • written 9 months ago by
Mark
▴ 60
2
votes
1
reply
795
views
Strategy to find transgene copy number and integration sites using only Nanopore simplex reads?
sequencing
transgenes
ngs
updated 10 months ago by
colindaven
7.9k • written 10 months ago by
Mark
▴ 60
1
vote
2
replies
1.1k
views
Why use Hi-C and/or Bionano for scaffolding when Nanopore sequencing exists?
wgs
hic
sequencing
nanopore
genomics
updated 10 months ago by
shelkmike
★ 1.8k • written 10 months ago by
Mark
▴ 60
5
votes
2
replies
787
views
Is there an easy way to scrape a whole bunch of NCBI RefSeq FASTA from NCBI?
FASTA
Genome
NCBI
updated 11 months ago by
shenwei356
8.7k • written 11 months ago by
Mark
▴ 60
0
votes
0
replies
931
views
Comment:
Comment: Basic question but how can I search the NCBI Nucleotide database using BLAST CLI
11 months ago by
Mark
▴ 60
2
votes
2
replies
931
views
Basic question but how can I search the NCBI Nucleotide database using BLAST CLI
cli
blast
11 months ago by
Mark
▴ 60
3
votes
4
replies
1.1k
views
Recommendations for performing Bulk RNA sequencing on non-model organism with poor genome annotation?
RNA-seq
updated 13 months ago by
Trivas
★ 1.9k • written 13 months ago by
Mark
▴ 60
0
votes
0
replies
944
views
Comment:
Comment: Does it make sense to use scRNAseq for this experiment?
15 months ago by
Mark
▴ 60
3
votes
2
replies
944
views
Does it make sense to use scRNAseq for this experiment?
scRNA-seq
15 months ago by
Mark
▴ 60
0
votes
0
replies
756
views
Comment:
Comment: Is there a program that can predict the sequence of an organisms telomeres just
18 months ago by
Mark
▴ 60
0
votes
2
replies
756
views
Is there a program that can predict the sequence of an organisms telomeres just from raw sequencing reads
telomeres
genomics
sequencing
18 months ago by
Mark
▴ 60
0
votes
0
replies
1.4k
views
Any tools for finding the telomere sequences / lengths directly for nanopore sequencing reads without any prior knowledge of the organism
genomics
telomeres
reads
22 months ago by
Mark
▴ 60
5
votes
4
replies
1.5k
views
Is there a good software to generate test genomics data?
genomics
testing
benchmarking
updated 22 months ago by
Brian Bushnell
20k • written 22 months ago by
Mark
▴ 60
0
votes
0
replies
1.4k
views
For people doing high molecular weight DNA sequencing library prep, do you see a steep drop off in the fragment sizes before and after the library pr…
library-prep
sequencing
ont
nanopore
ngs
22 months ago by
Mark
▴ 60
0
votes
0
replies
1.6k
views
Comment:
Comment: Best software to get summary statistics from nanopore sequencing runs
22 months ago by
Mark
▴ 60
4
votes
4
replies
1.6k
views
Best software to get summary statistics from nanopore sequencing runs
wgs
nanopore
sequencing
22 months ago by
Mark
▴ 60
2
votes
1
reply
770
views
Can someone help me explain this part of a lecture regarding gene finding HMM's
HMM
gene-prediction
updated 23 months ago by
Ram
45k • written 23 months ago by
Mark
▴ 60
0
votes
0
replies
589
views
Any way to predict hormone biosynthesis pathways in understudied organisms?
genomics
orthologs
biosynthesis-pathway
updated 2.0 years ago by
Ram
45k • written 2.0 years ago by
Mark
▴ 60
4
votes
5
replies
2.4k
views
For high quality Telomere to telomere assemblies, is short read polishing still necessary
genome
illumina
sequencing
nanopore
wgs
updated 2.0 years ago by
Buffo
★ 2.4k • written 2.0 years ago by
Mark
▴ 60
3
votes
2
replies
1.5k
views
Best pipeline / resources / tools for whole genome assembly in a haploid organism
haploid
sequencing
genomics
nanopore
wgs
updated 2.0 years ago by
Dave Carlson
★ 2.2k • written 2.0 years ago by
Mark
▴ 60
3
votes
2
replies
1.8k
views
Best pipeline / resources / tools for whole genome assembly
wgs
nanopore
Sequencing
updated 2.2 years ago by
Asaf
10k • written 2.2 years ago by
Mark
▴ 60
5
votes
3
replies
1.9k
views
how can i filter a sam file from a paf file
paf
sam
filter
updated 2.5 years ago by
ATpoint
89k • written 2.5 years ago by
Mark
▴ 60
1
vote
2
replies
2.3k
views
Best way to visualize .paf or .sam alignment to a fasta file
visualization
sam
fasta
paf
alignment
updated 2.5 years ago by
colindaven
7.9k • written 2.5 years ago by
Mark
▴ 60
0
votes
1
reply
883
views
Tool to identify mutations following global alignment?
Mutation
alignment
analysis
genomics
updated 2.6 years ago by
manaswwm
▴ 570 • written 2.6 years ago by
Mark
▴ 60
4
votes
4
replies
2.3k
views
Does anyone know how to create a sequence annotation figure like this?
genome
gene
sequence
annotation
chromosome
updated 3.7 years ago by
GenoTechUser
• 0 • written 3.7 years ago by
Mark
▴ 60
43 results • Page
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