13 hours ago by
analyst
▴
10
7
votes
17
replies
1.1k
views
5
votes
4
replies
6.0k
views
4
votes
5
replies
304
views
updated 9 hours ago by
colindaven
5.8k
•
written 1 day ago by
skilleta0527
•
0
4
votes
2
replies
155
views
4
votes
2
replies
190
views
4
votes
15
replies
6.4k
views
8 follow
4
votes
11
replies
6.0k
views
6 follow
updated 22 hours ago by
Yun
•
0
•
written 4.3 years ago by
ofek.schnitzer
▴
30
2
votes
4
replies
287
views
10 hours ago by
cucindarko51
•
0
2
votes
3
replies
117
views
updated 5 hours ago by
Pierre Lindenbaum
158k
•
written 5 hours ago by
awhale01
•
0
2
votes
4
replies
594
views
updated 23 hours ago by
wn835166087
•
0
•
written 3 months ago by
Konstantin
•
0
1
vote
1
reply
1.0k
views
updated 19 hours ago by
charles.feigin
•
0
•
written 6 weeks ago by
xinrantian
▴
20
1
vote
4
replies
128
views
updated 3 hours ago by
Alex Reynolds
35k
•
written 4 hours ago by
Mr Locuace
▴
150
1
vote
1
reply
160
views
1
vote
4
replies
200
views
1
vote
10
replies
1.8k
views
1
vote
2
replies
301
views
1
vote
5
replies
821
views
updated 19 hours ago by
charles.feigin
•
0
•
written 18 months ago by
jamesymtang
▴
10
0
votes
0
replies
4
views
0
votes
4
replies
657
views
0
votes
0
replies
70
views
0
votes
0
replies
63
views
19 hours ago by
junhuili
•
0
0
votes
2
replies
182
views
0
votes
4
replies
229
views
updated 11 hours ago by
GenoMax
136k
•
written 17 hours ago by
syedahumairagillani
•
0
0
votes
0
replies
56
views
Tutorial:
CITEseq tutorial
10 hours ago by
Ming Tommy Tang
★
3.6k
0
votes
1
reply
130
views
0
votes
1
reply
99
views
0
votes
1
reply
131
views
0
votes
2
replies
129
views
updated 8 hours ago by
Istvan Albert
99k
•
written 11 hours ago by
Manuel
•
0
0
votes
0
replies
688
views
0
votes
0
replies
46
views
4 hours ago by
mia.nahom
▴
10
0
votes
1
reply
74
views
updated 4 hours ago by
alex.zaccaron
▴
380
•
written 4 hours ago by
Simone
▴
10
0
votes
6
replies
239
views
3 hours ago by
sam.himes92
•
0
0
votes
1
reply
132
views
Recent Votes
Recent Locations •
All
Cambridge, MA, 2 minutes ago
Russia, 5 minutes ago
South Korea, 8 minutes ago
Egypt, 9 minutes ago
South Korea, 9 minutes ago
Hong Kong, 12 minutes ago
United States, 15 minutes ago
Recent Awards •
All
Popular Question to
samreen.jasvi
•
0
Scholar to
jared.andrews07
★
16k
Popular Question to
Mr Locuace
▴
150
Popular Question to
benjamin.pyenson
•
0
Popular Question to
Malachi Griffith
19k
Popular Question to
awhale01
•
0
Recent Replies
Comment: Resolving over clustered NGS with Q-scores
by
sam.himes92
•
0
The system that we used was [Aviti][1]. Our sequencing core was the one who quantified the loading/library concentration. Sorry I don't hav…
Comment: Where can I get a list of SNPs mapping overlapping genes in humans?
by
Alex Reynolds
35k
I modified the answer with an approach for that use case.
Comment: Where can I get a list of SNPs mapping overlapping genes in humans?
by
Ram
41k
OP is looking for overlapping genes - genes with presumably different gene IDs that share some loci. I think the `genes.bed` creation logic…
Comment: Where can I get a list of SNPs mapping overlapping genes in humans?
by
Alex Reynolds
35k
Note: The file `snps.bed` will be very large. You'll need sufficient disk space for this step.
Answer: Where can I get a list of SNPs mapping overlapping genes in humans?
by
Alex Reynolds
35k
Given files `genes.bed` and `snps.bed`, you could do something like:
```
$ bedmap --echo --echo-map-id --delim '\t' genes.bed snps.bed > a…
Answer: How to add exon annotations to genome annotation
by
alex.zaccaron
▴
380
You can use [AGAT][1] to process your annotation file to add the missing exons. I believe `agat_convert_sp_gxf2gxf.pl -g no_exons.gff -o wi…
Comment: Annotation Visualization IGV
by
Pierre Lindenbaum
158k
and you got answer that should be validate (green tick), or people asked you questions but got no answers.
Comment: Annotation Visualization IGV
by
Pierre Lindenbaum
158k
you asked questions before: review, comment and/or validate them please: https://www.biostars.org/p/9530777/ ; https://www.biostars.org/p/9…
Comment: Ambient RNA removal method that generates whole (integer) counts
by
EK
•
0
Thanks, Jared. Sounds like rounding counts is the simplest approach for now. :)
Comment: Resolving over clustered NGS with Q-scores
by
Trivas
★
1.5k
Could you tell us which sequencer you used, how you quantified your library size and concentration, and what your loading concentration was…
Comment: Resolving over clustered NGS with Q-scores
by
sam.himes92
•
0
I should have mentioned, we ran both Read 1 and 2 well past the points of interest. For each read we ran 75 cycles.
Answer: Resolving over clustered NGS with Q-scores
by
Trivas
★
1.5k
It might be because Illumina recommends 26 cycles for Read 1: https://knowledge.illumina.com/instrumentation/general/instrumentation-genera…
Comment: Resolving over clustered NGS with Q-scores
by
ATpoint
78k
Has phiX been spiked in to increase nucleotide diversity.
Traffic: 1343 users visited in the last hour
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by the
version 2.3.6
version 2.3.6