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122,232 results • Page
17 of 2445
Sort: Rank
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Votes
Replies
0
votes
2
replies
704
views
Discrepancy in RNA editing calls between STAR and Subjunc aligners using JACUSA2
jacusa2
rnaseq
subjunc
star
rsubread
4 months ago by
thomas.heigl.ibk
▴ 20
2
votes
1
reply
644
views
RNA-Seq paired-end datasets
paired-end
RNA-seq
Bioinformatic
updated 4 months ago by
Matthias Zepper
5.2k • written 4 months ago by
AIMAR
▴ 70
0
votes
0
replies
931
views
News:
Course: Introduction to CRISPR
GenomeEditing
CRISPR
Evolution
4 months ago by
Physalia-courses
★ 2.7k
4
votes
3
replies
994
views
Best strategy for comparing cell-type composition between disease and healthy groups in single-cell RNA-seq data
Single_cell
updated 4 months ago by
ATpoint
90k • written 4 months ago by
城玮
• 0
1
vote
4
replies
899
views
RNA-seq assigned alignments
paired-end
STAR
RNA-seq
mapping
single-end
updated 4 months ago by
jaro.slamecka
▴ 270 • written 4 months ago by
Manko47
▴ 10
0
votes
1
reply
536
views
Obtain Common SNPS for Mouse Genome
mouse
SNPs
updated 4 months ago by
GenoMax
154k • written 4 months ago by
joe_genome
▴ 70
1
vote
3
replies
2.1k
views
How do you clean GEO metadata before downstream analysis?
geo
updated 4 months ago by
yura.grabovska
▴ 890 • written 4 months ago by
Alba
• 0
1
vote
1
reply
2.0k
views
Seeking high-quality curated metabolic models with time-resolved flux data for sonification project
metabolism
sonification
networks
updated 4 months ago by
Mensur Dlakic
★ 30k • written 4 months ago by
omereilam1
• 0
0
votes
0
replies
438
views
Standard way to represent represent indel (insertion or deletion) alleles within a haplotype
haplotypes
indels
haploview
4 months ago by
Mwangana
• 0
1
vote
1
reply
3.4k
views
lovis4u Configuration
graph
lovis4u
genomics
updated 4 months ago by
GenoMax
154k • written 4 months ago by
NIkita
• 0
1
vote
1
reply
2.0k
views
Tool:
Tool to clean GEO metadata
GEO
metadata
updated 4 months ago by
yura.grabovska
▴ 890 • written 4 months ago by
Alba
• 0
1
vote
2
replies
3.7k
views
WGBS Analysis with 1 control sample and 6 disease samples
one
WGBS
sample
updated 4 months ago by
ATpoint
90k • written 5 months ago by
Cassandra
▴ 10
0
votes
2
replies
3.8k
views
How to extract gene list from GEO dataset in R
coding
beginner
question
r
updated 4 months ago by
Shohel
• 0 • written 5 months ago by
decorus
• 0
2
votes
6
replies
3.9k
views
RNA seq strandedness (reverse-strand)
seq
reverse-stranded
strand
RNA
4 months ago by
ZuelTech
• 0
0
votes
0
replies
383
views
TFs and enrichment analysis for TRAP sequencing
TRAP
4 months ago by
Genesis
• 0
0
votes
2
replies
3.5k
views
Extract track data from "search by region" feature on ENCODE
Encode
4 months ago by
AlienPumpkinPie
• 0
9
votes
8
replies
4.4k
views
Weird p-value distribution on edgeR results
edgeR
pvalue-distribution
gene-expression
updated 4 months ago by
dariober
15k • written 5 months ago by
Guillermo
▴ 30
1
vote
4
replies
3.6k
views
Draw GO tree for some GO ids with coloring
enrichment
GO
updated 4 months ago by
Istvan Albert
103k • written 4 months ago by
boczniak767
▴ 880
1
vote
4
replies
1.6k
views
Problem with Mirdeep2 and Randfold output
mirdeep2
Randfold
updated 4 months ago by
Jasim
• 0 • written 19 months ago by
otieno43
▴ 40
0
votes
1
reply
577
views
calculating coverage of gene in my metagenomes with samtools
samtools
depth
metagenome
coverage
updated 4 months ago by
Pierre Lindenbaum
166k • written 4 months ago by
lintonf
• 0
2
votes
1
reply
698
views
how to merge gbz files
vg
updated 4 months ago by
Jouni Sirén
▴ 800 • written 4 months ago by
lushjia
• 0
2
votes
1
reply
497
views
plotting read length distribution of Single End data
read_length_distribution
single_end_sequencing
WGS
4 months ago by
Meghan.T
▴ 10
4
votes
1
reply
582
views
Preranked GSEA permutation
GSEA
updated 4 months ago by
ATpoint
90k • written 4 months ago by
as823jk
• 0
2
votes
2
replies
3.5k
views
why bwa-mem only return one read with pair-read end
sequencing
bwa-mem
updated 4 months ago by
rfran010
★ 1.7k • written 4 months ago by
QX
▴ 80
0
votes
2
replies
557
views
Identification problem of IDE-8 cell on culture with inverted microscope
IDE-8
offtopic
updated 4 months ago by
i.sudbery
22k • written 4 months ago by
Prashanta
• 0
0
votes
2
replies
623
views
Identifying RBP motifs overlapping exons
motifs
bedtools
RBP
updated 4 months ago by
GenoMax
154k • written 4 months ago by
RK
• 0
0
votes
0
replies
893
views
News:
Upcoming NGS Data Analysis Courses 2025
SingleCell
RNAseq
Epigenomics
Illumina
DNAseq
4 months ago by
ecSeq Bioinformatics
▴ 20
0
votes
1
reply
564
views
How to match clinical information with expression data in R
R
updated 4 months ago by
Arup Ghosh
3.5k • written 4 months ago by
py3296746920
• 0
3
votes
1
reply
704
views
Identify SNP, Indel variants from a List of FASTA sequence
SNP
updated 4 months ago by
Mark
★ 1.7k • written 5 months ago by
Trinh
▴ 10
2
votes
19
replies
3.6k
views
Which Reference Hg38 or T2T?? Both??
Host
Removal
3 months ago by
Jon
• 0
3
votes
2
replies
671
views
Can I transduce HEK293T with Cas9 lentivirus followed by transfection with sgRNAs?
lentivirus
Transduction
CRISPR
transfection
offtopic
updated 4 months ago by
jared.andrews07
★ 19k • written 4 months ago by
louisflower1999
• 0
4
votes
4
replies
784
views
Mitochondrial annotation tool for algae
barcoding
annotation
18 days ago by
firefox91
▴ 10
1
vote
2
replies
2.2k
views
Identifying gene outliers in a genome using composition analysis.
RSCU
composition
GC
outliers
updated 4 months ago by
GenoMax
154k • written 4 months ago by
Shakunthala Natarajan
▴ 10
1
vote
5
replies
841
views
Mapping to a template sequece
sequencing
4 months ago by
QX
▴ 80
0
votes
2
replies
644
views
quality control for 16s metagenome reads
quality
metagneome
control
16s
fastp
updated 4 months ago by
GenoMax
154k • written 4 months ago by
m90
▴ 30
0
votes
3
replies
802
views
Loss of strandedness in the Illumina Strandedness Total RNA protocol
Illumina
RNA-seq
reads
stranded
4 months ago by
Anjan
▴ 850
0
votes
1
reply
821
views
Help using ShinyCell2 and Seurat v5 object.
shinycell
seurat
shiny
shinycell2
scRNA
updated 4 months ago by
antonioggsousa
3.4k • written 4 months ago by
Jamie
• 0
0
votes
0
replies
964
views
News:
Machine Learning for Multi-Omics Integration at the University of Barcelona– Dec 2025
Machine-Learning
Sulti-Omics
Depp-Learning
Data-Integration
BigData
4 months ago by
Physalia-courses
★ 2.7k
0
votes
0
replies
537
views
Compare alternative splicing patterns between human and mouse?
orthologs
rna-seq
splicing
alternative
4 months ago by
aboll
▴ 20
2
votes
6
replies
1.1k
views
Trouble getting representatives from clustered_nr database
database
blast
4 months ago by
Adolfo
• 0
0
votes
0
replies
393
views
FindTransferAnchors (reference prediction) vs Manual Annotations
Seurat
4 months ago by
reddyornah
• 0
3
votes
4
replies
1.5k
views
NCBI Datasets CLI Question
ncbi-datasets
4 months ago by
Bjorn
• 0
0
votes
0
replies
418
views
Incomplete assembly in HPRC dataset
pangenome
HPRC
reference
4 months ago by
ohell
• 0
4
votes
3
replies
995
views
Joint Calling for Large Germline WGS Cohort
NGS
RAM
cohort
Genomics
WGS
updated 4 months ago by
Jeremy Leipzig
23k • written 4 months ago by
j.k3096
▴ 20
2
votes
4
replies
834
views
how shoud the phred score be intepreted?
sequencing
updated 4 months ago by
GenoMax
154k • written 4 months ago by
QX
▴ 80
0
votes
3
replies
844
views
Inherited pipeline uses RNA-seq variant calls for WASP filtering—shouldn’t it be external SNPs?
WASP
updated 4 months ago by
i.sudbery
22k • written 5 months ago by
jonas.andersson
▴ 40
0
votes
0
replies
520
views
WGCNA analysis for Proteomics data
Proteomics
WGCNA
4 months ago by
Sumit Paliwal
▴ 40
3
votes
8
replies
7.5k
views
7 follow
Cellranger count pipestance failed: The read lengths are incompatible with all the chemistries
10x
cellranger
single-cell
updated 4 months ago by
zhang
• 0 • written 3.4 years ago by
firestar
★ 1.7k
1
vote
0
replies
571
views
Can iHS and XP-EHH be applied to targeted sequencing data?
sequencing
targeted
ngs
ihs
5 months ago by
slzr_
▴ 10
2
votes
3
replies
1.0k
views
PacBio amplicon reads partially aligned using minimap2 – library or analysis issue?
PacBio
minimap2
alignment
variants
pbmm2
4 months ago by
louisflower1999
• 0
122,232 results • Page
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Comment: Data integration single cell using Harmony
Answer: Feature Counts vs Salmon quantification
Answer: Chip-seq analysis Diffbind
Question about variant calling method using pangenome-graph
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Answer: Is this Rosalind inspired project actually useful?
Answer: Minimap2 segfaults when mapping Nanopore reads to a very large reference databas
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Comment: "sortmerna" quitting early--is this is a memory issue?
by
Andrew
• 0
Ok, here's the output: https://pastebin.com/uEHSckHS This run had the working directory in the default location, in sortmerna\run, but it …
Answer: Problems in Molecular Docking in Autodock vina
by
Anonsirs
• 0
Try to re run the command by first starting meeko and rdkit . to convert PDB files to PDQT files. It can help vina recognize the atom . a…
Comment: "sortmerna" quitting early--is this is a memory issue?
by
Andrew
• 0
Do you mean the maximum % RAM usage it reaches right before the moment it quits? As I said, the computer has 16 GB RAM, and the run fails w…
Comment: Chip-seq analysis Diffbind
by
Irene
▴ 10
Thank you very much for always answering my doubts and questions. I truly value and appreciate it because it helps me understand the result…
Comment: Minimap2 segfaults when mapping Nanopore reads to a very large reference databas
by
firefox91
▴ 10
Many thanks !
Answer: Re-assembling old RNA-seq reads vs. using an existing transcriptome assembly
by
GenoMax
154k
> I have access to both the raw reads and the previously assembled transcriptome submitted to TSA. and > I am trying to determine wheth…
Answer: Re-assembling old RNA-seq reads vs. using an existing transcriptome assembly
by
dthorbur
★ 3.2k
There are a few factors to consider: **The quality of the existing reference.** If there is a high quality chromosome level assembly,…
Comment: "sortmerna" quitting early--is this is a memory issue?
by
colindaven
8.1k
Pls add the output and your system (especially RAM), no one here can guess.
Answer: Power calculations for differentially methylated DNA samples from sequencing
by
colindaven
8.1k
There are software packages for power analysis such as https://academic.oup.com/bioinformaticsadvances/article/5/1/vbaf150/8173951.
Comment: Data integration single cell using Harmony
by
Arup Ghosh
3.5k
Run QC for each sample separately, then merge run SCTransform, PCA, then Harmony, UMAP, FindNeighbors and FindClusters.
Comment: Data integration single cell using Harmony
by
_deb
• 0
Thank you for the quick answer. I still have a doubt. It would be better to pre-process each sample separately (*filtering, SCTransform…
Comment: Issue while running Kenddata.
by
rana.elromh
• 0
I checked the memory before executing this command. I made a bash script on Ubuntu to run it on 15 folders. But, this problem happened in f…
Comment: Prokka output genes clusterization by function
by
Mensur Dlakic
★ 30k
Many proteins have multiple domains, and are correctly classified with multiple KO numbers. That's not a bug in `kofam_scan` but rather a r…
Comment: mapping sequences on specific region of bacteria genome
by
samuel.a.odonnell
▴ 650
If you want to visualise the mapping; take a look at using IGV. It just requires the genome you used to map the reads and the BAM output (n…
Comment: Prokka output genes clusterization by function
by
shevch2009
▴ 20
Thanks, I had completely forgotten that METABOLIC works with protein sequences. That will work :) I was just trying to figure out how peop…
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