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33 results • Page
1 of 1
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0
votes
1
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18
views
Low mapping percentage
mapping
STAR
RNAseq
updated 20 minutes ago by
GenoMax
129k • written 1 hour ago by
Sib
▴ 40
0
votes
0
replies
16
views
Visualization App for RNASeq Differential Expression and Enrichment Analysis
differential
visualization
RNASeq
analysis
expression
enrichment
1 hour ago by
Mark
• 0
0
votes
0
replies
15
views
calculate p value and associated z score for snp-gene pair
snp
association
gene
pvalue
2 hours ago by
rheab1230
▴ 140
0
votes
0
replies
47
views
busco id description
OrthoDB
BUSCO
updated 4 hours ago by
Ram
39k • written 5 hours ago by
hembrom.angelina
• 0
0
votes
5
replies
162
views
how to Construct a Newick tree file from five large fasta files
tree
Newick
alignment
updated 40 minutes ago by
Joe
21k • written 9 hours ago by
gunala.nikhil
• 0
0
votes
0
replies
53
views
NMDS plotting issue
NMDS
metagenomic
6 hours ago by
arshad1292
▴ 90
0
votes
1
reply
74
views
featurecounts not working on mirbase annotation file
usegalaxy
miRNA-seq
updated 53 minutes ago by
GenoMax
129k • written 6 hours ago by
demoraesdiogo2017
▴ 90
0
votes
0
replies
50
views
IGV insertions
insertions
IGV
8 hours ago by
christoph.schubart
• 0
0
votes
0
replies
55
views
Bayesian network for biological data using bnlearn
bnlearn
RNA-seq
bayesian-network
updated 4 hours ago by
Ram
39k • written 8 hours ago by
priyankamehta.1811
• 0
0
votes
0
replies
46
views
jellyfish options
kmer
jellyfish
8 hours ago by
Tele
• 0
0
votes
3
replies
98
views
hardfilter error
bam
recall
8 hours ago by
bestone
▴ 10
0
votes
0
replies
42
views
cnetplot enrichment graph to cytoscape
clusterprofiler
cnetplot
enrichplot
9 hours ago by
Omics data mining
▴ 220
0
votes
0
replies
46
views
How to extract the surrogate variables from DNA methlation Data
sva
array
limma
methylation
updated 9 hours ago by
GenoMax
129k • written 10 hours ago by
Dablax
• 0
1
vote
1
reply
157
views
Reads with highest MAPQ values from SAM files are showing mismatches to reference sequence and IGV classified them as supplementary reads
minimap
Nanopore
IGV
updated 9 hours ago by
GenoMax
129k • written 10 hours ago by
Mohd
▴ 20
0
votes
0
replies
43
views
Stratified sample assignment for analysis
experimental-design
stratification
updated 4 hours ago by
Ram
39k • written 10 hours ago by
fr
▴ 200
0
votes
0
replies
39
views
Convert tree into bifurcation table
Tree
Newick
ASR
Mutation
10 hours ago by
Alexandre
• 0
0
votes
1
reply
86
views
Processing WES VCF for case control GWAS analysis
GWAS
PLINK
updated 8 hours ago by
raphael.B
▴ 360 • written 12 hours ago by
sonsunjirachote
• 0
0
votes
4
replies
151
views
convert data frame with character column to data frame with integer column
r
51 minutes ago by
atemedorri137798
• 0
4
votes
6
replies
1.5k
views
Why weblogo of biopython doesn't work?
Biopython
Weblogo
updated 18 hours ago by
minakshiboruahassam
• 0 • written 9 months ago by
Plus
▴ 20
1
vote
2
replies
142
views
The famous WGS dataset, Ashkenazi Trio?
wgs
dataset
updated 4 hours ago by
thadjudkins2
• 0 • written 19 hours ago by
herh
• 0
0
votes
1
reply
94
views
RNASeq gene labeling and mRNA filter from bulkRNA data.
RNA-Seq
RSubreads
updated 14 hours ago by
ATpoint
72k • written 19 hours ago by
Yeeshouw
• 0
1
vote
0
replies
93
views
Integrating mRNA and microRNA analysis results
mRNA
microRNA
22 hours ago by
abba647
▴ 10
0
votes
1
reply
112
views
Merge different vcf files
vcf
updated 3 hours ago by
Ram
39k • written 1 day ago by
caique.manochio
• 0
0
votes
4
replies
217
views
Identification of genes involved in my pathway
KEGG
keggGet
23 hours ago by
smanzano250800
• 0
0
votes
8
replies
287
views
The inchworm process failed. Trinity running error.
inchworm
transcriptome
trinity
updated 9 hours ago by
GenoMax
129k • written 1 day ago by
Marta
• 0
0
votes
1
reply
675
views
Small demo bioinformatics workflows which don't require reference data?
workflow
pipeline
updated 36 minutes ago by
Ram
39k • written 3.7 years ago by
steve
★ 3.3k
4
votes
8
replies
1.6k
views
How to extract promoter sequences from a plant draft genome?
promoter
genome
updated 20 minutes ago by
Ram
39k • written 3.7 years ago by
Kumar
▴ 100
0
votes
3
replies
2.4k
views
Generate GTF/GFF file (coordinates) from a FASTA annotated file.
Assembly
genome
updated 43 minutes ago by
Ram
39k • written 3.7 years ago by
marquezg48
• 0
0
votes
1
reply
802
views
Filtering for homozygous reference alleles
SNP
sequence
genome
updated 49 minutes ago by
Ram
39k • written 3.7 years ago by
AGE
▴ 30
0
votes
1
reply
746
views
plasmid spades R1/R2 files?
plasmid
sequencing
updated 49 minutes ago by
Ram
39k • written 3.7 years ago by
sarah.goldstein
• 0
3
votes
7
replies
1.1k
views
Find 3'UTRs for species
Assembly
alignment
updated 1 hour ago by
Ram
39k • written 3.8 years ago by
Palgrave
▴ 110
0
votes
0
replies
584
views
Proportion of traits in several groups
propotion-test
fishers-exact-test
updated 2 hours ago by
Ram
39k • written 3.8 years ago by
biobiu
▴ 140
0
votes
0
replies
533
views
Online courses/studies bioinformatics
online-study
learning
updated 2 hours ago by
Ram
39k • written 3.8 years ago by
MAR
• 0
33 results • Page
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Recent Votes
Answer: What does canonical transcript mean in the context of VEP
Comment: Optimum setting for local blastp for ~10K sequences
Perl. How to extract gene entry, CDS and gene sequence from a specified range from GenBank files?
Comment: How to sort gff3 according to chromosome order?
Answer: How to sort gff3 according to chromosome order?
Comment: Does adding reads cause batch effects?
Answer: Optimum setting for local blastp for ~10K sequences
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Recent Replies
Comment: How to sort gff3 according to chromosome order?
by
Pierre Lindenbaum
154k
-s, --stable stabilize sort by disabling last-resort comparison
Comment: Low mapping percentage
by
GenoMax
129k
If you do have contamination (either from rRNA of same species or from true contaminant(s)) there is not much you can do about that at this…
Comment: Optimum setting for local blastp for ~10K sequences
by
GenoMax
129k
> Running the same sequence on the blast web server took 10 seconds Don't try to compare anything local with NCBI's web blast infrastruct…
Comment: how to Construct a Newick tree file from five large fasta files
by
Joe
21k
Yes, I know - but that doesn't answer the question. **What *kind* of phylogenetic tree?**
Comment: Optimum setting for local blastp for ~10K sequences
by
sodiumnitrate
▴ 20
Thank you for the explanation! I managed to get diamond to work, but I'm having trouble getting it to run faster than blast. For a single t…
Comment: What is "pident" (percentage of identical matches) in the "Diamond" protein alig
by
GenoMax
129k
That is the question @Vincent answer poses since it seems to correspond to something different. You can also create an issue on https://gi…
Answer: How to sort gff3 according to chromosome order?
by
GenoMax
129k
Please use `AGAT`: https://agat.readthedocs.io/en/latest/topological-sorting-of-gff-features.html
Comment: convert data frame with character column to data frame with integer column
by
atemedorri137798
• 0
dear @Basti : As you said in order to clarify my issue I edit question box with give an example. I will be grateful if you take a look at …
Comment: How to sort gff3 according to chromosome order?
by
Ram
39k
Those are your options with the `sort` utility - you can either keep the existing order or re-order by coordinate. If you're looking for a …
Comment: How to sort gff3 according to chromosome order?
by
BioinfoBee
• 0
using sort -k1,1 doesn't keep the order of child features of each gene in output.
Comment: How to sort gff3 according to chromosome order?
by
Ram
39k
You're sorting by chromosome AND co-ordinates. Try just `sort -k1,1`
Comment: How to sort gff3 according to chromosome order?
by
BioinfoBee
• 0
it does sort but not able to keep the child features (mRNA, CDS, exon) in proper order. For example, I can sort using: sort -k1,1 -k4,4n -k…
Comment: How to sort gff3 according to chromosome order?
by
Pierre Lindenbaum
154k
uh ? what about the [sort][1] command ? [1]: https://man7.org/linux/man-pages/man1/sort.1.html
Comment: What does canonical transcript mean in the context of VEP
by
Ram
39k
> Does that mean the fully functional gene most folks think of when thinking of their favorite gene? That ... doesn't make much sense. Did…
Answer: What does canonical transcript mean in the context of VEP
by
Pierre Lindenbaum
154k
https://www.ensembl.org/info/genome/genebuild/canonical.html > The Ensembl Canonical transcript is a single, representative transcript ide…
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