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You didn't mention what kind of organism you are working with and what kind of protein you are looking for?
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Thank you Gordon for your expertise. One question that I have now that I have a list of DE genes is how to interpret logFC of this continuo…
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Yes I plan to use QIIME2, but I'm Cutadapting outside QIIME so its easier to MultiQC without importing/unzipping QZAs (and QIIME2 importing…
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> 3' adapters were removed aprox. 90 times each one, so there sohuld not be read-through at all here
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Following your advice, I checked the primer and adapters sequences and edited the question accordingly
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Fungal ITS2 region (length is really variable so read-through can be an issue)
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Also try the NCBI AWS bucket transfer tool. It's extremely convenient, however it's not instantaneous and you need to have a (paid) AWS acc…
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