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1,061 results • Page
1 of 22
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0
votes
3
replies
37
views
Easy way to find out which allele is minor allele from bed file?
plink
updated 2 minutes ago by
bk11
★ 2.4k • written 1 hour ago by
curious
▴ 750
0
votes
0
replies
8
views
vcf procesing for ML antigen prediction
formats
vcf
fastaq
HLA_imputation
HLA_typing
40 minutes ago by
Javier
• 0
0
votes
0
replies
12
views
Extracting mutation status from MAF files - TCGA
TCGA
maf
mutation
1 hour ago by
yahn
• 0
0
votes
0
replies
14
views
IGV interpretation for ATM gene for chromosome 11 for a deceased partient suspected of having melanoma
genetic
Variant
predisposition
1 hour ago by
mikemakaveli1
• 0
0
votes
0
replies
21
views
Tissue-specific DEG analysis with DEseq2
DEseq2
RNA-seq
DEG
R
updated 1 hour ago by
Ram
43k • written 1 hour ago by
M.
▴ 30
0
votes
2
replies
47
views
Raw counts using stringtie
stringtie
RNA-seq
updated 36 minutes ago by
GenoMax
142k • written 2 hours ago by
ahmad.sajad4541
• 0
0
votes
3
replies
92
views
Differential Expression Analysis using DESeq2 in R
deseq2
R
updated 1 hour ago by
GenoMax
142k • written 3 hours ago by
Erina
• 0
0
votes
0
replies
22
views
Modify plot R mtDNA indel
plot
mtDNA
R
2 hours ago by
marco.barr
▴ 90
0
votes
0
replies
24
views
Re-Import modified clusterProfiler's GSEA result from .csv for graphical output-generation (i.e. cnetplot, heatplot)
enrichplot
graphical_output
GSEA
clusterProfiler
3 hours ago by
NorbertK
• 0
2
votes
2
replies
99
views
Analysis of intronic reads included scRNA-seq data
cell
single
updated 3 hours ago by
t.montserrat.ayuso
▴ 10 • written 6 hours ago by
carolofharvest
▴ 30
9
votes
9
replies
377
views
High Malat-1 expression in single cell data
cell
analysis
single
updated 44 minutes ago by
dsull
★ 6.0k • written 4 days ago by
carolofharvest
▴ 30
0
votes
1
reply
72
views
Downloading older version of a tool
Alignment
tools
updated 4 hours ago by
GenoMax
142k • written 4 hours ago by
Ruqaiya
• 0
0
votes
0
replies
42
views
Generating illumina interops SAV like plots for multiqc reports on HPC
visualization
illumina
ngs
sequencing
5 hours ago by
rustykb
▴ 20
1
vote
1
reply
67
views
DSEQ2 analysis
DSEQ2
logfoldchane
updated 6 hours ago by
ATpoint
82k • written 6 hours ago by
adi.gershon1
• 0
3
votes
5
replies
153
views
Generating mpileup file using samtools
mpileup
samtools
updated 4 hours ago by
Joe
21k • written 7 hours ago by
Ruqaiya
• 0
0
votes
5
replies
224
views
6 follow
What purposes can TPM values be used for?
RNA-seq
TPM
Normalization
DESeq2
updated 8 hours ago by
b.contreras.moreira
▴ 180 • written 1 day ago by
JH
• 0
1
vote
3
replies
167
views
Does comparing two different groups to a common third group introduce bias in the analysis?
DESEQ
RNAseq
updated 8 hours ago by
ATpoint
82k • written 18 hours ago by
Qi
• 0
0
votes
0
replies
49
views
News:
Cellosaurus release 49 is available
cell
biocuration
line
database
news
knowledgebase
9 hours ago by
Amos Bairoch
▴ 120
1
vote
3
replies
126
views
Help with choosing a model species for Augustus for a de novo assembled genome.
augustus
annotation
assembly
genome
updated 1 hour ago by
dthorbur
★ 1.9k • written 10 hours ago by
Vijith
▴ 30
0
votes
0
replies
68
views
SIngle cell analysis
Seurat
scRNA-seq
Harmony
DoubletFinder
Merge
11 hours ago by
Jeyong
• 0
0
votes
2
replies
653
views
homer not configured properly
Homer
makeTagDirectory
updated 13 hours ago by
clairechung112
• 0 • written 23 months ago by
amahdi779
• 0
1
vote
3
replies
165
views
Bedtools merge minimum overlap?
bedtools
genomics
updated 14 hours ago by
Alex Reynolds
35k • written 20 hours ago by
SJP
• 0
0
votes
0
replies
64
views
Empty table plot using plotGseaTable()
FGSEA
18 hours ago by
Chris
▴ 260
0
votes
1
reply
109
views
VG : No reference-sense paths available in the graph; falling back to generic paths.
vg
updated 20 hours ago by
anovak
▴ 120 • written 1 day ago by
Hang
• 0
1
vote
3
replies
157
views
DiffBind: no peaks in DBA
MACS2
DiffBind
ChIP-seq
updated 20 hours ago by
jared.andrews07
★ 16k • written 1 day ago by
yvonneh
▴ 10
1
vote
2
replies
133
views
Converting CRAM to FastQ
GATK
samtools
cram
SamToFastq
fastq
updated 20 hours ago by
GenoMax
142k • written 20 hours ago by
Maverick
▴ 10
3
votes
8
replies
6.5k
views
7 follow
GATK genomicsDBimport intervals for WGS
gatk
genomicsdbimport
updated 21 hours ago by
Sd
• 0 • written 5.1 years ago by
Nicolas Rosewick
11k
0
votes
2
replies
120
views
Base Quality Score Recalibration with different reference genome
SNPs
GATK
BQSR
RNA-seq
21 hours ago by
dtnondorf
• 0
0
votes
1
reply
147
views
Spike-in control found in raw reads (16S amplicon seq) but not picked up by DADA2 - where to go from here?
nf-core
16S
amplicon
dada2
ampliseq
updated 13 hours ago by
Chris Dean
▴ 390 • written 22 hours ago by
sovrappensiero
▴ 90
1
vote
2
replies
168
views
RNA-seq data for deep learning classification
rna-seq
4 hours ago by
yahn
• 0
2
votes
6
replies
266
views
How to access TCGA samples that were treated with a specific drug?
TCGA
RNA-seq
16 hours ago by
Qroid
▴ 40
0
votes
1
reply
467
views
Loss of 'var' using concatenation of AnnData objects
Scanpy
AnnData
updated 1 day ago by
Hugo
• 0 • written 4 months ago by
Rachel
• 0
0
votes
1
reply
104
views
Provean help
variant
Provean
updated 21 hours ago by
Mensur Dlakic
★ 27k • written 1 day ago by
Arun Sai Kumar
• 0
1
vote
1
reply
122
views
Download eQTL data of one specific gene for all tissues from GTEx
eQTL
GTEx
updated 1 day ago by
bk11
★ 2.4k • written 1 day ago by
Jeol
▴ 20
1
vote
1
reply
131
views
Super ehancers
enhancers
updated 1 day ago by
jared.andrews07
★ 16k • written 1 day ago by
Oburah
• 0
0
votes
6
replies
217
views
How does gene length effect the number of reads mapped
RNA-seq
CPM
sequencing
updated 2 hours ago by
dsull
★ 6.0k • written 1 day ago by
Chen
• 0
5
votes
5
replies
217
views
Problem with data downloaded from Short Reads Archive (SRA)
SRA
DADA2
metabarcoding
updated 1 day ago by
atharvakarkare14
▴ 30 • written 1 day ago by
Begonia_pavonina
▴ 150
1
vote
7
replies
400
views
Downsampling long-read BAM files
nanopore
BAM
QC
ONT
downsampling
5 hours ago by
eebloom
▴ 80
3
votes
3
replies
237
views
What analysis suitable to identify similarly expressed genes between two samples (treated vs untreated). Contrary to DGE analysis,
Gene-Expression
RNA
NOISeq
DGE
updated 6 hours ago by
ATpoint
82k • written 1 day ago by
alifafiq1
• 0
0
votes
0
replies
79
views
Use of annotation of integrated Seurat object in single sample
Seurat
1 day ago by
Bine
▴ 60
0
votes
0
replies
86
views
News:
Beyond beginner R: Advancing your coding skills - September 16-19
Advanced-Coding
Programming
R
updated 1 day ago by
Ram
43k • written 1 day ago by
carlopecoraro2
★ 2.5k
0
votes
1
reply
202
views
Subsetting and merging back Seurat Object brings different results
Seurat
1 day ago by
Bine
▴ 60
0
votes
0
replies
107
views
Job:
Postdoctoral Position in Bioinformatics - Mainz, Germany
NGS
Genomics
Post-doc
R-loops
updated 1 day ago by
Ram
43k • written 1 day ago by
4r-rtg
• 0
1
vote
1
reply
124
views
Discrepancy in read counts from fastq
downsampling
fastq
updated 1 day ago by
Ram
43k • written 1 day ago by
marco.barr
▴ 90
0
votes
0
replies
77
views
edgeR contrasts for analysis of reciprocal hybrid crosses - averaging, pooling, and comparing groups
edgeR
1 day ago by
Rhiannon
• 0
0
votes
0
replies
88
views
Successfully identified and Masked rpeats using RM. What Next?
sequence
annotation
repeatmasker
illumina
assembly
1 day ago by
Vijith
▴ 30
9
votes
10
replies
442
views
6 follow
Bacterial plasmid analysis
bacteria
plasmid
wgs
hybridassembly
sequencing
updated 1 day ago by
Zamin Iqbal
▴ 20 • written 1 day ago by
nicole.kavanagh
• 0
0
votes
1
reply
263
views
Is there a way to increase the automatic label text size in Cytoscape?
Cytoscape
updated 1 day ago by
Scooter
▴ 280 • written 7 days ago by
avocado123
• 0
0
votes
0
replies
87
views
Combining Methylation Microarray data from different platforms
Microarray
DNA
EPIC
450K
Methylation
1 day ago by
James
▴ 10
0
votes
1
reply
575
views
Basic stats in hierfstat
Fst
stats
hierfstat
updated 1 day ago by
mdav
• 0 • written 18 months ago by
Zoe
• 0
1,061 results • Page
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Recent Votes
Answer: Does comparing two different groups to a common third group introduce bias in th
Comment: High Malat-1 expression in single cell data
Comment: High Malat-1 expression in single cell data
Print Differentially Expressed Exons From Dexseq Results
C: Print Differentially Expressed Exons From Dexseq Results
C: Print Differentially Expressed Exons From Dexseq Results
Answer: Please Explain Window Size and Step Size
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Recent Replies
Comment: Easy way to find out which allele is minor allele from bed file?
by
curious
▴ 750
Yes, but I think the problem is what to do when MAF = 0.5
Answer: Easy way to find out which allele is minor allele from bed file?
by
bk11
★ 2.4k
In Plink, A1 is usually a minor allele and A2 a major allele. .frq (basic allele frequency report) Produced by --freq. …
Comment: Raw counts using stringtie
by
GenoMax
142k
> Error parsing strand from GFF line Looking at this error and the listing of files in your directory it looks like you need to `gunzip` t…
Comment: High Malat-1 expression in single cell data
by
dsull
★ 6.0k
I briefly looked at those genes on a genome browser -- there are a lot of repeat elements in those genes, meaning a lot of counts assigned …
Comment: High Malat-1 expression in single cell data
by
carolofharvest
▴ 30
Thank you for sharing. What are your thoughts on the genes Gm42418, AY036118, and Gm26917? Some articles suggest that clusters with an abu…
Comment: Do I need to go back and filter my long-reads?
by
GenoMax
142k
> I think I will filter the reads ultimately. If your data was not run and basecalled using the same exact version of pore/software for a…
Comment: Raw counts using stringtie
by
Ram
43k
What is `stringie.sh`? Try `./stringtie.sh -h` and then `more stringtie.sh`.
Comment: Do I need to go back and filter my long-reads?
by
Ram
43k
> I think you should have the right to delete a question you yourself have submitted If you were to talk to someone, can you make them for…
Comment: Help with choosing a model species for Augustus for a de novo assembled genome.
by
dthorbur
★ 1.9k
I don't have any experience using `BRAKER2` so I cannot comment, but I would be extra careful about data you find on non-model organisms. T…
Comment: Differential Expression Analysis using DESeq2 in R
by
GenoMax
142k
You can copy and paste your code and then use `101010` button in the edit window to format it as text to keep it monospaced. For example …
Comment: Same sequencing sample in multiple lanes. How to analyse it?
by
ST
• 0
I guess this could really depend on the "counting" strategy, the library complexity and possible lane biases. If e.g., EM is used to guide…
Comment: Help with choosing a model species for Augustus for a de novo assembled genome.
by
Vijith
▴ 30
Hi dthorbur, thanks for the response. I have run Augustus using Arabidopsis as a species. I would like to know about the suitability of usi…
Comment: How does gene length effect the number of reads mapped
by
dsull
★ 6.0k
Alicia Oshlack has done some cool work on this but even the plots in her papers show that TPMs sometimes overcorrect and the relationship i…
Comment: Differential Expression Analysis using DESeq2 in R
by
Erina
• 0
![The below image contains the code and result also.][1] [1]: /media/images/96562b96-d508-4f67-96d1-7c71aa17
Comment: High Malat-1 expression in single cell data
by
dsull
★ 6.0k
My response above was actually partly inspired off of a reading of your paper :)
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