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Comment: CuffDiff hangs at same point
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chavanrenuka852
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hi i have the same issue can you please provide me details how to create small ban file or how to provide subset of bams?
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Mensur Dlakic
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I don't think anyone can help you until you tell us the error code.
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Don't know about GO and KEGG, but pretty sure there is no FASTA database for KOG.
BLAST annotation is decidedly old-school, which I don't …
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Hi there, I have the same problem. did you success using SNPchimpRepo? Thanks!
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stringsAsBaxters
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Did you ever solve this?
GraphAligner doesn't retain the base quality scores in the GAM output, see:
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Hi, rob, thanks to your reply and I wrote a demo in Rust. Repo here: https://github.com/dwpeng/kmer
Comment: If changing the RNA-Seq count normalisation method changes the DGE and downstrea
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analyst
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Okay thank you so much for making things clear. Raw counts were used for DGE analysis in DESeq2.
Thanks a lot for saving time dsull!
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OK, since you mention you're using DESeq2 for DGE analysis.
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Thanks dsull, I am using deseq2 for DGE analysis
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How are you performing the DGE analysis / what tool are you using for it?
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Can anyone tell me why there is NNNNNN= no of gaps present in the final fasta file while converting bam into fasta with samtools consensus …
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After conversion i got multiple NNNNN in the final fasta file. can anyone tell be why this happens
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Thank you for the detailed answer. I will read KMC' and cuttlefish's source code.
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Have you looked at this issue in GitHub https://github.com/10XGenomics/cellranger/issues/133
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I often see a small population of B cells expressing CD3D in scRNAseq data, and I also do find some papers discussing this phenomenon.
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