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89 results • Page
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Votes
Replies
3
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2
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404
views
Recommended way to normalize SmartSeq2 gene expression matrix to better match 10X expression data
rna-seq
smartseq2
r
updated 3 days ago by
yhdist
▴ 70 • written 5 days ago by
Cookin
• 0
0
votes
0
replies
142
views
Why we are using filtering >0 for up and <0 for down after TopTags() to extract de genes ids?
EdgeR
logfc
DEG
5 days ago by
Ann
▴ 10
0
votes
1
reply
162
views
PyMOL: how to list all selection names?
pymol
updated 5 days ago by
Wayne
★ 1.8k • written 5 days ago by
Ondina
▴ 90
2
votes
2
replies
201
views
Clustering algorithm based on grouping sequences into gene families
gene-families
clustering
updated 4 days ago by
Ram
40k • written 5 days ago by
francesco
• 0
0
votes
0
replies
132
views
Homozygous reference genotype for a GIAB genome
GIAB
IDs
reference
rs
Homozygous
6 days ago by
New2R
▴ 60
0
votes
0
replies
137
views
PRSice - Phenotype File Not Read Correctly
PRSice2
6 days ago by
V
• 0
1
vote
5
replies
474
views
Idat raw data conversion
idat
updated 5 days ago by
Giulio Genovese
▴ 330 • written 7 days ago by
Zi
• 0
1
vote
3
replies
339
views
What is "intersectional genetic strategy"? How does it work? What can it do?
cell-subtypes
6 days ago by
Ethan Lee
• 0
0
votes
0
replies
175
views
What metrics to use to calculate variant library evenness or uniformity or bias
library-bias
variant
updated 6 days ago by
Ram
40k • written 7 days ago by
eli_bayat
▴ 90
2
votes
4
replies
414
views
Help with celltype annotation
seurat
single-cell
6 days ago by
Chris
▴ 180
3
votes
4
replies
340
views
Weighted analysis
RNA-seq
4 days ago by
Peter
• 0
1
vote
2
replies
255
views
Multiple testing adjustment for stepwise model selection
statistics
5 days ago by
mel22
▴ 100
0
votes
0
replies
167
views
PopGenome: there are missing regions when calculating Tajima's D per gene
PopGenome
5 days ago by
Bing
• 0
1
vote
2
replies
270
views
functional analysis prediction
functionalanalysis
metabarcoding
16S
5 days ago by
safeassli
• 0
0
votes
8
replies
727
views
Chipseq analysis on repeat genes
galaxy
repeatmasker
ChIP-seq
repeat-elements
ATAC-seq
1 day ago by
pb11
▴ 10
0
votes
5
replies
441
views
Convert gene id's to gene symbol preserving gene id's in deseq2
ensembl
r
DE
deseq2
updated 6 days ago by
Ram
40k • written 8 days ago by
dylannicoembros
• 0
4
votes
5
replies
467
views
Pre-processing for single cell RNAseq: Hard thresholds, data (cluster)-driven or both?
scRNA-seq
5 days ago by
psm
▴ 100
0
votes
3
replies
295
views
RdRp scan - identifying/detecting viruses- metagenomic workflow- need help
RdRp
virus-detection
metagenomic
updated 4 days ago by
Ram
40k • written 9 days ago by
eric--carron
• 0
2
votes
2
replies
253
views
Pangenome using Orthofinder
OrthoFinder
Pangenome
bacteria
5 days ago by
kirankumareripogu
▴ 10
0
votes
6
replies
842
views
FACS (Fluorescence-activated cell sorting NOT cyTOF),Automating the removal of dead and duplicated cells
FACS
2 days ago by
ccbb7aab4
▴ 20
0
votes
3
replies
261
views
Extracting/assembling organellar reads from RNAseq data
transcriptome
star
alignment
mapping
updated 5 days ago by
LChart
3.3k • written 12 days ago by
Miles
• 0
1
vote
3
replies
257
views
Question regarding MACS2 approach of peak calling
MACS2
sequencing-depth
chipseq
updated 3 days ago by
ATpoint
76k • written 12 days ago by
rkc5
• 0
0
votes
11
replies
688
views
Normalization for microarrays >1000+ samples?
microarray
normalization
oligo
3 days ago by
evmae
• 0
0
votes
3
replies
327
views
RSubread DESeq2
RNA-seq
subread
differential-expression
deseq2
updated 5 days ago by
Ram
40k • written 14 days ago by
Beyza
• 0
3
votes
4
replies
411
views
During alignment STAR has stopped at started alignment step
STAR
updated 3 days ago by
Ram
40k • written 6 weeks ago by
Santhi
• 0
1
vote
10
replies
875
views
Deepvariant variant calling by singularity
deepvariant
singularity
conda
docker
updated 11 hours ago by
jeffhsu3
• 0 • written 11 weeks ago by
fra.silvestro99
▴ 10
1
vote
0
replies
319
views
Strand orientation and GC skew
CGView
strand-orientation
GC-skew
updated 2 days ago by
Ram
40k • written 13 months ago by
A_heath
▴ 140
0
votes
1
reply
376
views
Close genome comparison
Mauve
Mugsy
genome-comparison
GSAlign
updated 2 days ago by
Ram
40k • written 19 months ago by
A_heath
▴ 140
8
votes
5
replies
1.6k
views
BLASTp command line : filtering results by a minimum % of identity and % coverage
BLASTp
updated 2 days ago by
Ram
40k • written 23 months ago by
A_heath
▴ 140
2
votes
3
replies
1.5k
views
Racon command line with paired-ends Illumina reads
Illumina
Racon
updated 2 days ago by
Ram
40k • written 2.0 years ago by
A_heath
▴ 140
0
votes
0
replies
356
views
Distinction of two very close bacterial strains
mutations
bacteria
updated 2 days ago by
Ram
40k • written 2.3 years ago by
A_heath
▴ 140
1
vote
5
replies
664
views
Mugsy percentage of identity
Mugsy
Genome-alignment
updated 2 days ago by
Ram
40k • written 2.5 years ago by
A_heath
▴ 140
0
votes
3
replies
724
views
How to add translation of CDS in Artemis?
translation
Artemis
updated 2 days ago by
Ram
40k • written 2.7 years ago by
A_heath
▴ 140
0
votes
1
reply
754
views
How to identify specific region using Mauve?
Mauve
primers
updated 2 days ago by
Ram
40k • written 2.8 years ago by
A_heath
▴ 140
0
votes
7
replies
779
views
Mugsy error -directory must be a directory
Mugsy
updated 2 days ago by
Ram
40k • written 2.8 years ago by
A_heath
▴ 140
0
votes
1
reply
898
views
Issue interpreting plasmidSPAdes output
plasmidSPAdes
updated 2 days ago by
Ram
40k • written 2.9 years ago by
A_heath
▴ 140
0
votes
0
replies
588
views
Contig extension using PRICE
price
updated 2 days ago by
Ram
40k • written 3.0 years ago by
A_heath
▴ 140
1
vote
3
replies
667
views
How to determine plasmid or chromosomal origin?
plasmid
chromosomal-origin
updated 2 days ago by
Ram
40k • written 3.0 years ago by
A_heath
▴ 140
0
votes
3
replies
2.0k
views
extraction of original gene iDs from reference annotation file
rna-seq
updated 3 days ago by
aishwarya
• 0 • written 5.2 years ago by
blooming.daisy333
▴ 110
89 results • Page
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Recent Votes
Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
Comment: Error in bowtie2
Recommended Tools For Alternative Splicing Detection From Rna-Seq Data
Comment: Using STAR aligner to build index of hg38
Answer: How to sort using samtools
The Biostar Handbook. A bioinformatics e-book for beginners.
The Biostar Handbook. A bioinformatics e-book for beginners.
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Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Y
• 0
I tried the commands you recommended in the bash/command line. When I tried `find "${bam_directory}" -type f -name "*.bam" | xargs samtools…
Comment: Hugo_Symbol to Entrez ID
by
GenoMax
134k
Can you provide some examples of HUGO ID's you are unable to convert?
Comment: Using STAR aligner to build index of hg38
by
GenoMax
134k
GTF file includes gene models/annotation information. You could also use a program like `salmon/kallisto` with just human transcriptome to…
Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Pierre Lindenbaum
157k
then test each files. find "${bam_directory}" -type f -name "*.bam" | xargs samtools quickcheck file "${reference_gen…
Answer: Ties in reranked list
by
alserg
▴ 840
The problem here is not the ties, but that your gene IDs in the pathway list (ensemble human genes) does not match the names of the stats v…
Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Y
• 0
I did not use the space between the `-u` flag and the `-o` flag (the blank line) but I did do what you recommended: # Run CuffDiff wit…
Answer: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Pierre Lindenbaum
157k
how about simply trying: ``` cuffdiff -u "${annotation_gtf}" \ -o "${output_directory}" \ -b "${reference_genome}" \ -p "${num_th…
Comment: Using STAR aligner to build index of hg38
by
Grace
• 0
Yes, I know also need a GTF file. So the GTF file will include RNA information? Thanks for your answer!!
Comment: STAR Intron Motif Script Gives Segmentation fault Error
by
Y
• 0
I will try and figure it out on my own given what you all have mentioned. Thank you for your time.
Comment: Using STAR aligner to build index of hg38
by
Amitm
★ 2.2k
Have you gone through the manual [here][1]. In addition to the genome fasta file, you would also need a GTF file (of gene annotations) to b…
Comment: Highly inflated p-values in GWAS by regenie
by
4galaxy77
2.8k
You still definitely need to include principle components as covariates, even if your data is from the same ethnicity. Not including those …
Answer: How to create structural variants ground truth for alignment of two long-read ge
by
Christophe
• 0
Hi, D-genies is using minimap2 to align both genomes and minimap2 is chaining local alignments to produce a global one. If the SV are sm…
Comment: Ties in reranked list
by
Hamza
• 0
In this case there are no results in my fgsea object unfortunately
Comment: Genes with promoter and enhancer regions as GTF
by
abis.1819104
• 0
how to generate promoters.bed and enhancer.bed files from gtf file
Comment: Deepvariant variant calling by singularity
by
jeffhsu3
• 0
I am having a similar issue was this ever resolved? Getting this error: I0922 22:22:08.794076 140356687390528 make_examples_core.py:257] O…
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