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121,890 results • Page
15 of 2438
Sort: Rank
Rank
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Votes
Replies
1
vote
1
reply
465
views
Decision making on choosing the based on tblastn result
interpretation
tblastn
BLAST
E-score
updated 3 months ago by
GenoMax
153k • written 3 months ago by
srestha
• 0
9
votes
7
replies
3.3k
views
How to Identify the pathogenicity of a set of variants (by using ACMG guidelines)?
pathogenicity
SNP
variants
updated 3 months ago by
Damianos P. Melidis
▴ 60 • written 6.8 years ago by
DanielC
▴ 210
0
votes
4
replies
853
views
Why am I getting the exact same MarkDuplicates results when passing a query or coordinate sorted bam?
picard
bwa
updated 3 months ago by
GenoMax
153k • written 3 months ago by
curious
▴ 890
0
votes
2
replies
537
views
use AverageExpression to stratify samples
scRNA
3 months ago by
ndra1456
• 0
0
votes
0
replies
1.1k
views
Multiple taxonomy hits per OTU ID in VSEARCH despite --top_hits_only (90% ID after QIIME2 clustering at 97%)
vsearch
updated 3 months ago by
GenoMax
153k • written 3 months ago by
salma.sarker
• 0
0
votes
0
replies
432
views
RepeatMasker output file question
RepeatMasker
3 months ago by
xinguok794
• 0
5
votes
1
reply
808
views
Which GWAS tool to use that can work on Czech cases and British controls, on a binary trait?
plink2
gwas
saige
updated 3 months ago by
LChart
5.1k • written 3 months ago by
anita.szabo08
▴ 10
1
vote
2
replies
618
views
Unsure whether or not I overcorrected for batch effect in dataset
Quality
Plot
PCA
Control
updated 3 months ago by
GenoMax
153k • written 3 months ago by
Megan
▴ 60
0
votes
3
replies
700
views
Primer removal in amplicon sequencing
removal
Primer
Amplicon
DADA2
sequencing
updated 3 months ago by
GenoMax
153k • written 3 months ago by
sxf520
• 0
1
vote
1
reply
976
views
GATK BQSR error — Reference and BAM file chromosome name mismatch (“chr” vs. no “chr”)
GATK
VCF
BSQR
updated 3 months ago by
Ram
45k • written 3 months ago by
iamsmor
• 0
0
votes
1
reply
579
views
Batch correction without biological covariates
Combat-seq
RNA-seq
batch-correction
updated 3 months ago by
Ram
45k • written 3 months ago by
as823jk
• 0
2
votes
3
replies
689
views
RagTag results
NGS
scaffolding
RagTag
gaps
updated 3 months ago by
shelkmike
★ 1.8k • written 3 months ago by
synthiiihuh
• 0
0
votes
2
replies
570
views
Tools that output positions of matching kmers
sequences
genomics
kmers
3 months ago by
gil.hornung
▴ 100
2
votes
4
replies
736
views
mitochondrial assamply use NOVOplasty
mitochondrial
genome
mammles
updated 3 months ago by
cfos4698
★ 1.1k • written 3 months ago by
m90
▴ 30
0
votes
3
replies
663
views
Still bad phred score after fastp
phred
fastp
updated 3 months ago by
Arup Ghosh
3.4k • written 3 months ago by
SpliceAndScript
• 0
0
votes
1
reply
503
views
Cytohubba problem and difference networks
Network
cytoscape
cytohubba
analysis
updated 3 months ago by
Scooter
▴ 310 • written 3 months ago by
mastorehpournazari
• 0
0
votes
1
reply
678
views
Cytoscape KEGG plots reverting to original layout after changing style or column
kegg
cytoscape
updated 3 months ago by
Scooter
▴ 310 • written 4 months ago by
Adrian
• 0
6
votes
6
replies
1.0k
views
Mass-spectrometry proteomics
Proteomics
analysis
10 weeks ago by
Nasim Gandomdoust
• 0
8
votes
3
replies
762
views
Biological meaning of metrics commonly used for QC in scRNA-seq?
ribosome
QC
velocity
single-cell
intron
updated 3 months ago by
jared.andrews07
★ 19k • written 3 months ago by
txema.heredia
▴ 250
0
votes
0
replies
506
views
News:
Eukaryotic eDNA Metabarcoding Course (14–18 July, Online)
Metabarcoding
eDNA
Genomics
Nanopore
3 months ago by
Physalia-courses
★ 2.6k
7
votes
12
replies
1.3k
views
miRNA low mapping(Qiagen miRNA Library Prep)
Qiagen
pipeline
trimming
alignment
miRNA
updated 3 months ago by
GenoMax
153k • written 3 months ago by
anthony.santana.703.j
▴ 30
0
votes
12
replies
1.8k
views
STAR alignment not detecting some transcripts / custom reference
star
bwa
rna-seq
alignment
scrna-seq
updated 3 months ago by
rfran010
★ 1.6k • written 3 months ago by
npont
▴ 20
0
votes
0
replies
404
views
eHOMD database: Ref seq Aligned FASTA vs Full RefSeq?
HOMD
database
3 months ago by
bioinfo
▴ 60
2
votes
3
replies
837
views
pysam header and body
bam
pysam
view
updated 3 months ago by
dariober
15k • written 3 months ago by
Matteo Ungaro
▴ 130
4
votes
1
reply
639
views
Discrepency in assembly sizes
Pacbio
assembly
Kmer
updated 3 months ago by
shelkmike
★ 1.8k • written 3 months ago by
hpapoli
▴ 170
0
votes
1
reply
533
views
fasterq-dump fails before completing lookup
lookup
incomplete
fasterq-dump
updated 3 months ago by
GenoMax
153k • written 3 months ago by
nmannda148
• 0
0
votes
4
replies
754
views
ATACSEQ normlization for bigwig files
normalization
atac-seq
updated 3 months ago by
ATpoint
89k • written 3 months ago by
RD
▴ 30
6
votes
6
replies
902
views
finding gene sequence from WGS data
gene
WGS
updated 3 months ago by
swbarnes2
15k • written 3 months ago by
analyst
▴ 70
0
votes
0
replies
418
views
Question about Protein-protein-DNA complex prediction
prediction
protein
3 months ago by
mickley413
• 0
1
vote
10
replies
2.5k
views
Supergnova stuck in tutorial
Genetic
Correlations
Python
Supergnova
Conda
updated 3 months ago by
s.faria
• 0 • written 3.0 years ago by
Gerard
▴ 10
2
votes
4
replies
1.1k
views
RNAseq meta-analysis to identify “consistently expressed” genes
meta-analysis
method
rnaseq
updated 3 months ago by
mbyvcm
▴ 480 • written 3 months ago by
cgibbsm
▴ 20
1
vote
2
replies
615
views
pddg+odgi+vg to perform pangenome analysis to cyanobacteria
odgi
pggb
help
pangenome
vg
updated 3 months ago by
GenoMax
153k • written 3 months ago by
snmdba
▴ 10
1
vote
2
replies
610
views
Comparing sets of genes for similarity
R
jaccard
genes
marker
clustering
3 months ago by
yura.grabovska
▴ 820
0
votes
0
replies
513
views
News:
Network Analysis in Systems Biology - 2 seats left
Bioconductor
SystemsBiology
R
NetworkAnalysis
RNAseq
3 months ago by
Physalia-courses
★ 2.6k
2
votes
5
replies
805
views
Running Unicycler with a merged fastq file
unicycler
bacterial-genome
assembly
updated 3 months ago by
GenoMax
153k • written 3 months ago by
Assa Yeroslaviz
★ 1.9k
3
votes
5
replies
905
views
Assemblers for short read bacterial isolates data
data
isolates
assembly
shotgun
updated 3 months ago by
Mensur Dlakic
★ 30k • written 3 months ago by
shevch2009
▴ 20
0
votes
2
replies
607
views
Biopython get_terminals() doesn't get all terminals
biopython
tree
get_terminals
gtdb
3 months ago by
khp35
• 0
0
votes
0
replies
447
views
Covariate Selection for DESeq2/ANCOM-BC: Alpha vs. Beta Diversity Findings
ANCOM-BC
microbiome
covariates
DESeq2
PERMANOVA
3 months ago by
bioinfo
▴ 60
0
votes
1
reply
863
views
NF core rna-seq pipeline
nf-core
nextflow
pipeline
rnaseq
updated 3 months ago by
GenoMax
153k • written 3 months ago by
rajdeepboral00
▴ 70
0
votes
0
replies
431
views
Need Access to RadGraph2 Dataset from PhysioNet — Stuck at CITI Course Step
dataset
3 months ago by
z.mohammadi64
• 0
6
votes
3
replies
742
views
Both up- and down-regulated gene sets return the same GO pathway analysis results?
DEseq2
GO-analysis
3 months ago by
xqyn
▴ 60
0
votes
1
reply
561
views
Discrepancies calculatin average coverage with Bedtools and Mosdepth
mosdepth
human
bedtools
libraries
illumina
updated 3 months ago by
Pierre Lindenbaum
166k • written 3 months ago by
María José
▴ 10
4
votes
7
replies
4.2k
views
Michigan imputation server error
TOPMed
VCF
updated 3 months ago by
Joel Wallenius
▴ 220 • written 5.2 years ago by
AR
• 0
2
votes
8
replies
1.1k
views
Proposal on CNV (Copies Variants Numer) analysis tools
Number_Variant
Somatic_data
Help
Copy
3 months ago by
AIMAR
▴ 70
2
votes
2
replies
634
views
Error with bigWigAverageOverBed
usc
human
libraries
illumina
bigWigAverageOverBed
updated 3 months ago by
Pierre Lindenbaum
166k • written 3 months ago by
María José
▴ 10
9
votes
13
replies
1.3k
views
de novo chromosome level assembly
genome
assembly
chromsome
updated 3 months ago by
colindaven
7.9k • written 3 months ago by
mthm
▴ 80
6
votes
2
replies
591
views
How is PLINK obtaining p-values and coefficients for linear regression
PLINK
GWAS
updated 3 months ago by
GenoMax
153k • written 3 months ago by
sakura
▴ 40
3
votes
4
replies
665
views
bcftools not filtering snps
bcftools
3 months ago by
analyst
▴ 70
4
votes
2
replies
596
views
There are a way to align phage reads in to bacterial genomes?
alignment
sequences
reads
updated 3 months ago by
GenoMax
153k • written 3 months ago by
schlogl
▴ 180
4
votes
5
replies
929
views
No index in the undetermined fastq file
bcl2fastq
3 months ago by
1769mkc
★ 1.3k
121,890 results • Page
15 of 2438
Recent Votes
Answer: Stringtie de novo VS reference guided (-G)
Answer: Yeast reference-based genome assembly
How to Identify Conserved 21-mers Across TE Insertions in Drosophila?
How to Identify Conserved 21-mers Across TE Insertions in Drosophila?
Answer: Rna-seq data
Answer: What Are Your Most-Used Public Data Repositories?
Answer: activity of unloaded/unassembled Tn5
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Panos
★ 1.8k
Popular Question
to
amy967107
• 0
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Chris
▴ 360
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Michael
56k
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Andrew Su
5.0k
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bioinformatics_rk
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e.r.zakiev
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Recent Replies
Comment: Yeast reference-based genome assembly
by
amy967107
• 0
I have ~2.01 Gb of ONT data and the target genome is ~20–23 Mb (90–100× coverage). My ONT read N50 is ~3.8 kb, so contiguity may be limited…
Comment: Yeast reference-based genome assembly
by
amy967107
• 0
This is a yeast strain (probably Rhodotorula spp.) and I'm trying to assemble the entire genome from nanopore reads using a reference-based…
Answer: Yeast reference-based genome assembly
by
Michael
56k
The new [hifiassm ONT][1] may be all you need for getting a good assembly. I would not recommend LRSDAY, except for its annotation part (wh…
Comment: magpurify errors
by
Mensur Dlakic
★ 30k
By the way, these errors are not unique. If you look through the `magpurify` GitHub issues, both types have been reported already. - https…
Comment: magpurify errors
by
Mensur Dlakic
★ 30k
There is absolutely no chance that a contig labeled `k127_4584534` doesn't exist in your fasta files. These names are not made up out of th…
Comment: magpurify errors
by
shevch2009
▴ 20
Strange but there are no such headers or contig names in my fasta files
Comment: replace gene names in gff file
by
Juke34
9.3k
Yes the --prefix Option exists. There is no count per chromosome. You can do it by splitting your input and analyse one sequence at a time…
Comment: Variant analysis for mouse WES
by
GenoMax
153k
You could open a new issue and ask the developers of `maftools` if this was something they can add.
Comment: How to Identify Conserved 21-mers Across TE Insertions in Drosophila?
by
GenoMax
153k
Do you have the locations/sequence of the regions you are interested in?
Comment: magpurify errors
by
shevch2009
▴ 20
Thank you
Comment: magpurify errors
by
Mensur Dlakic
★ 30k
To me that sounds like an error in sequence header formatting. Maybe some contig headers are repeated? Or their sequences are short or miss…
Comment: magpurify errors
by
shevch2009
▴ 20
Thanks What about phylo-markers module error, I tried to look for it :) But haven't found anything. And why it's not working for some but …
Answer: magpurify errors
by
Mensur Dlakic
★ 30k
A general answer is to always Google the error message. Hopefully you do that in the future. This is the outcome of Googling `AttributeErro…
Comment: Splice junctions plots for long-read seq data
by
LChart
5.1k
IGV can view coverage and splice junction sashimi plots. However the plot in the paper looks like it was made with miso. https://miso.readt…
Comment: Yeast reference-based genome assembly
by
GenoMax
153k
> The gene originates from yeast, What does this mean? Are you putting a gene from a different genus/species into some other yeast? Are yo…
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