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121,906 results • Page 33 of 2439
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1
vote
3
replies
711
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cannot install GO.db in windows
GO.db
updated 6 months ago by 22k • written 6 months ago by • 0
1
vote
5
replies
1.1k
views
conda :FileNotFoundError: [Errno 2] No such file or directory
conda
updated 6 months ago by ★ 30k • written 6 months ago by • 0
6
votes
5
replies
873
views
Samtools view only showing read2 for PE mapped reads
Samtools mem2 bwa flagstat mapping
updated 6 months ago by 166k • written 6 months ago by • 0
0
votes
3
replies
1.5k
views
Color AlphaFold PDB by B-Factor (pLDDT) in PyMol
pdb pymol
updated 6 months ago by ★ 30k • written 6 months ago by ▴ 10
2
votes
2
replies
1.1k
views
How to Resolve Overlapping Peaks in Sanger Chromatograms?
sequence-analysis sanger-sequencing dna-sequencing chromatogram
6 months ago by JOSÉ • 0
2
votes
3
replies
1.0k
views
How to remove rows with empty gene symbols from a seurat object?
seurat
updated 6 months ago by 56k • written 7 months ago by ▴ 360
4
votes
12
replies
1.6k
views
7 follow
Phylogenetic tree from whole genome using BUSCO
BUSCO whole_genome phylogenetic_tree
6 months ago by manaswiniparija3 ▴ 60
0
votes
0
replies
437
views
News: Course on Ancient Metagenomics - online, 27-29 May
ancientDNA Metagenomics aMeta
7 months ago by Physalia-courses ★ 2.6k
0
votes
1
reply
859
views
Difference between trim galore report and Fastqc pre trimming
trim-galore
updated 6 months ago by 45k • written 3.2 years ago by • 0
0
votes
0
replies
322
views
From Cram to gVCF
Cram gVCF
7 months ago by heureuse ▴ 10
2
votes
4
replies
2.5k
views
R CytoTRACE2 Results: Potency Score of Naive B Cells Lower Than Memory B and Plasmablasts – Contradicts Biological Knowledge
CytoTRACE2
7 months ago by Binghong ▴ 20
3
votes
1
reply
664
views
Rules for Assigning Start and End Phase to Coding Exons
stare_phase reading_frame end_phase
updated 7 months ago by ▴ 30 • written 7 months ago by ▴ 20
0
votes
1
reply
542
views
converting transcript positions to genomic locations
genomic locations
updated 7 months ago by 166k • written 7 months ago by ▴ 60
0
votes
1
reply
552
views
Read groups RNA-seq and hisat2
hisat2 RNA-seq read-groups
updated 7 months ago by 45k • written 7 months ago by • 0
0
votes
0
replies
386
views
simulate a genome with specific event
simulation genome retrocopy
7 months ago by Matteo Ungaro ▴ 130
6
votes
4
replies
1.3k
views
6 follow
Help with R programming assignment
R offtopic
updated 6 months ago by 56k • written 14 months ago by • 0
1
vote
1
reply
1.1k
views
Job: SciLifeLab PULSE: Program for Future Leaders in Life Science (48 positions!)
Entrepreneur Sweden Mentoring Postdoctoral Leadership
7 months ago by Matthias Zepper 5.1k
1
vote
1
reply
646
views
MSA with extended alphabet
Multiple sequence MSA alignment
updated 7 months ago by ★ 30k • written 7 months ago by • 0
0
votes
0
replies
314
views
Help with Finding SNPs in H. pylori Assembled Genomes
question Technical
7 months ago by vrao • 0
0
votes
2
replies
653
views
Reference Genome with decoy for RNAseq data processing
STAR SALMON RNAseq
updated 7 months ago by ★ 1.3k • written 7 months ago by ▴ 40
1
vote
1
reply
539
views
multiple ATG SEQUENCE
MULTIPE sequence ATG offtopic
updated 7 months ago by 9.0k • written 7 months ago by • 0
0
votes
0
replies
330
views
Keep SNP's with call rate greater than 30 in VCF
SNP call rate ROH genotyping
updated 7 months ago by 6.5k • written 7 months ago by • 0
9
votes
10
replies
6.3k
views
7 follow
Buy PC for metagenomics
Assembly alignment genome next-gen
updated 7 months ago by 22k • written 7.2 years ago by ▴ 10
0
votes
0
replies
348
views
Dynamic tree cut results
clustering DEG
7 months ago by Mai.Nabil • 0
0
votes
0
replies
333
views
Significant variation in FastQ File Sizes - WES
LibraryPrep WES Human
7 months ago by irate.pirate • 0
0
votes
2
replies
811
views
Repetitive sequences analysis
Repetitive sequences
updated 7 months ago by ★ 1.6k • written 7 months ago by • 0
0
votes
1
reply
554
views
Protein secuence extraction from gpff file for a genes list
Protein gpff
updated 7 months ago by 13k • written 7 months ago by ▴ 300
1
vote
0
replies
972
views
Job: Postdoctoral Researcher in Single-Cell Sequencing Analysis
researcher post-doctoral
7 months ago by ejvandenoord ▴ 10
1
vote
2
replies
723
views
filtering heterozygous genotypes
bcftools heterozygous
7 months ago by analyst ▴ 70
2
votes
0
replies
462
views
An alternative to haplotype phasing - assembly?
samtools assembly BAM spades phasing
7 months ago by Jess ▴ 60
0
votes
0
replies
307
views
how to make/get the SNP annotation file for running TORUS
TORUS Annotation SNP GWAS
updated 7 months ago by 153k • written 7 months ago by • 0
1
vote
1
reply
512
views
Flongle 16srRNA nanopore sequencing
R.9.4.1 flow flongle
updated 7 months ago by 153k • written 7 months ago by • 0
1
vote
2
replies
740
views
Genomes with existing T2T assemblies?
T2T genome assembly reference
updated 7 months ago by ★ 4.3k • written 7 months ago by ★ 2.1k
1
vote
2
replies
1.0k
views
Installing ggpubr in Docker container rocker/r-base??
ggpubr dockerfile docker r
updated 7 months ago by 45k • written 7 months ago by ▴ 30
0
votes
0
replies
468
views
News: Bayesian Data Analysis in R course - online, March 3-7
Bayesian Statistics R
7 months ago by Physalia-courses ★ 2.6k
4
votes
5
replies
1.0k
views
Should we cite sed / awk in papers?
sed awk
updated 7 months ago by 89k • written 7 months ago by ▴ 250
0
votes
0
replies
294
views
Briggs model parametes
Briggs damage Gargammel
7 months ago by anna.utili • 0
3
votes
10
replies
1.5k
views
Nanopore sequencing - plasmid assembly length confusion
assembly long nanopore reads
6 months ago by abedkurdi10 ▴ 190
0
votes
0
replies
361
views
Post-GWAS colocalization analysis VS. variant annotation, what's the difference?
variant annotation snp colocalization GWAS
6 months ago by sarfdezco • 0
0
votes
2
replies
532
views
EDTA ,Transposon detection, repeat modeler and masker
homology_based_TE_detection fragmentedTE
6 months ago by manaswiniparija3 ▴ 60
2
votes
8
replies
1.3k
views
Finding ORFs in a FASTA file
MatrixScore ORFs Motifs MotifScoring
updated 7 months ago by 56k • written 7 months ago by • 0
2
votes
3
replies
683
views
Ilumina Low quality bases at first 20 cycles + PHIX problem
Sequencing
updated 7 months ago by 153k • written 7 months ago by ▴ 10
1
vote
3
replies
590
views
HMP dataset download issue (403 error)
HMP dataset microbiome
updated 7 months ago by 153k • written 7 months ago by ▴ 70
0
votes
1
reply
397
views
Enrichment Analysis for cCREs
Enrichment statstest bedtools cCREs fishers
updated 7 months ago by 89k • written 7 months ago by • 0
1
vote
0
replies
826
views
Job: Postdoctoral Research Position: Developing Novel Algorithms and Tools for Circular RNA Analysis
circRNAs biology bioinformatics computational
7 months ago by Tobias Jakobi ▴ 10
8
votes
10
replies
1.8k
views
Per Sequence GC Content fail
fastqc
5 months ago by Eduardo ▴ 20
8
votes
7
replies
7.4k
views
has anyone use trimmomatic with NEBNext adaptors
rna-seq
updated 7 months ago by ▴ 80 • written 6.8 years ago by ▴ 10
3
votes
1
reply
524
views
t2t RNAseq alignment with STAR
chm13 t2t RNAseq
updated 7 months ago by 153k • written 7 months ago by ▴ 30
2
votes
0
replies
19k
views
Herald: The Biostar Herald for Wednesday, February 19, 2025
herald
7 months ago by Biostar 3.6k
2
votes
3
replies
735
views
Cell Type annotation showing Normal cells as Cancer cells in single cell analysis.
seurat Single_cell spatial-transcriptomics
updated 7 months ago by ★ 1.6k • written 7 months ago by ▴ 20
121,906 results • Page 33 of 2439
Recent Votes
Answer: Tried building a compact sequence format with 4-bit storage
Tried building a compact sequence format with 4-bit storage
Shift in RNA 3' position, Term-Seq vs RNASeq
Comment: STARlong for pacbio Isoseq reads
Comment: Automatically annotating a feature using a genbank file
Answer: Is this PCA plot correct??
Answer: Is this PCA plot correct??
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Recent Replies
Comment: Is total miRNA in EV constant? Implications for library size normalization. by Gordon Smyth ★ 8.3k
RNA-seq is very good at relative analyes. It would be straightforward to determine which miRNAs are relatively more abundant, compared to o…
Comment: Tried building a compact sequence format with 4-bit storage by Pranava ▴ 10
Woah!!! Thank you very much, Matthias. I honestly didn't even know some of those existed and I will definitely go through them and try to u…
Answer: Tried building a compact sequence format with 4-bit storage by Matthias Zepper 5.1k
Hello Pranava, Thanks for sharing your repository. Devising a new file format is an interesting undertaking and certainly a task with many…
Answer: Gene Ontology Enrichment with LOC gene IDs in Common Carp (Cyprinus carpio) by GenoMax 153k
`LOC` gene ID's are often assigned to uncharacterized genes and you may not get useful information from databases for them, thus they will …
Comment: Whole Genome Sequencing IG regions by GenoMax 153k
> are the calls I am seeing reliable or should I take them with something of a pinch of salt? Not sure what calls you are referring to but…
Comment: RNA-SEQ where only a subset of genes is of interest by piffelpaff • 0
Sorry to revive this ancient thread but I'm currently discussing the same question with some people in my lab and would love to somebody po…
Answer: STARlong for pacbio Isoseq reads by YuZJ • 0
Hello, you may try deSALT (DOI: 10.1186/s13059-019-1895-9, GitHub: ydLiu-HIT/deSALT) or uLTRA (DOI: 10.1093/bioinformatics/btab540, GitHub:…
Comment: Shift in RNA 3' position, Term-Seq vs RNASeq by acvill ▴ 350
Without knowing the details of the Term-seq method, could the 38 nt shift be accounted for by the difference in the position of the ribosom…
Comment: STARlong for pacbio Isoseq reads by Buffo ★ 2.4k
Thanks, I'll give it a try.
Comment: STARlong for pacbio Isoseq reads by GenoMax 153k
You could give `mapPacBio.sh` from BBMap suite a try while you wait for other answers. That works for reads up to 6 kb. BBMap has a new hom…
Comment: STARlong for pacbio Isoseq reads by Buffo ★ 2.4k
The average is 1.8kb. I was planning to post it on GitHub, but it seems like the repository has been unattended for the last months, so I d…
Comment: Getting just fastqc.zip file? by GenoMax 153k
Yes, You can either use it on command line or use the graphical user interface.
Comment: STARlong for pacbio Isoseq reads by GenoMax 153k
What is the average length of your reads? That error sounds like a bug, so you may want to post that on `STAR` repo as an issue. I assume …
Comment: Getting just fastqc.zip file? by pourhangleila95 • 0
I want to perform fastQC on my data.Do you use fastQC software for doing it?
Comment: Whole Genome Sequencing IG regions by 719 • 0
Sorry for the poor wording of the original post, also should say I really appreciate people taking a moment to look at my post. My main con…
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