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Recent Replies
Answer: NGS forensics: how to know if data is fabricated
by
Mensur Dlakic
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27k
I share the opinion by @philippbayer I have seen enough questionable things in life and in science to keep me permanently jaded, but there…
Comment: How many reads for WGS Sequencing?
by
Mensur Dlakic
★
27k
I downloaded the data in two files from the link you provided. With this command:
megahit -1 ERR072246_1.fastq.gz -2 ERR072246_2.fastq…
Comment: How many reads for WGS Sequencing?
by
Mensur Dlakic
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27k
If you have Linux the assembly should work on your system, but 8 GB is generally not enough for assembling larger genomes.
Comment: How many reads for WGS Sequencing?
by
Mensur Dlakic
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27k
I think you might be getting stuck on less relevant parts of my exercise. The most important point was that nothing is wrong with the data.…
Comment: What does it mean single base resolution in sequencing?
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dsull
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5.9k
Great comprehensive explanation and overview of the technologies -- with regards to sequencing assays, the one thing I'd add is SNV identif…
Answer: What does it mean single base resolution in sequencing?
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dsull
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I think that term means slightly different things in different contexts. For example, it's useful to profile mRNA modifications (e.g. m6a) …
Comment: Cannot process all the reads in a fast5 file?
by
chujie
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Hi, I met the same problem, have you found the solution?
Answer: What does it mean single base resolution in sequencing?
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LauferVA
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Hi @fe3e6f65 ,
In a single sentence, the answer to your question can be summarized as, "*the resolution of a genomic technology refers to …
Comment: PCA plot
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LauferVA
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Without more context, we cannot interpret this PCA plot. There are many possibilities. In addition, this question is basic enough that it w…
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Jeremy Leipzig
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i have the name ready: out**liar**
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GenoMax
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Sequencing by hybridization (https://en.wikipedia.org/wiki/Sequencing_by_hybridization ) is likely not giving you single base resolution. B…
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Hii... i have updated the script in python..But, still getting no data found. Actually the data is there, i have print the parsed html file…
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ATpoint
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https://bioconductor.org/books/release/OSCA.multisample/using-corrected-values.html
Comment: Downloading full alignments from Pfam
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GenoMax
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Not as convenient but the entire set of full alignments can be downloaded here: https://ftp.ebi.ac.uk/pub/databases/Pfam/current_release/Pf…
Comment: How many reads for WGS Sequencing?
by
GenoMax
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> What does clipped Fastq mean?
Probably means that NCBI has already scanned and trimmed adapter sequencers.
> both forward and revers…
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