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173 results • Page
4 of 4
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Replies
5
votes
4
replies
377
views
Forum:
Ideal PC configurations and operating system for bioinformatics laboratory
PC
4 days ago by
Estevão
• 0
2
votes
4
replies
294
views
from row count to tpm
tpm
row-count
normalization
updated 6 days ago by
Ram
43k • written 14 days ago by
michelafrancesconi9
▴ 20
1
vote
8
replies
479
views
Downsampling fastq file
downsample
fastq
6 days ago by
marco.barr
▴ 90
0
votes
10
replies
530
views
Low mapping rate with Salmon
RNA-seq
Salmon
Quantification
updated 6 days ago by
i.sudbery
19k • written 14 days ago by
Patadu94
• 0
1
vote
6
replies
341
views
Do I need to go back and filter my long-reads?
alignment
nanopore
filtering
QC
ONT
updated 3 days ago by
GenoMax
142k • written 21 days ago by
eebloom
▴ 80
1
vote
0
replies
189
views
News:
Landscape Genomics course in Switzerland
LFMM
Landscape-Genomics
Sambada
R
Local-Adaptation
4 days ago by
carlopecoraro2
★ 2.5k
1
vote
2
replies
280
views
read length in structural variant calling
nanopore
SV
QC
ONT
variant
3 days ago by
eebloom
▴ 80
1
vote
10
replies
836
views
error to run rnammer
phylogenomics
rRNA_extraction
rnammer
updated 11 hours ago by
antonio.spl
• 0 • written 3 months ago by
microorganism_001
▴ 30
4
votes
15
replies
1.6k
views
how to test for differential expression in samples where a global increase in gene expression is expected
edgeR
DESeq
Differential-Expression
RNA-Seq
updated 2 days ago by
Gordon Smyth
★ 7.1k • written 4 months ago by
raplayer
▴ 10
1
vote
4
replies
1.1k
views
Filtering qscore on dorado
dorado
filtering
QC
nanopore
Guppy
4 days ago by
eebloom
▴ 80
29
votes
28
replies
34k
views
11 follow
Split Fastq Files Into Chunks Of 1M Reads
split
fastq
updated 4 days ago by
thomas.heigl.ibk
• 0 • written 12.8 years ago by
Bioscientist
★ 1.7k
5
votes
6
replies
1.8k
views
agilent human all exon sureselct v7 bed file
exon
human
bed
v7
agilent
all
sureselect
updated 1 day ago by
MiladAD
▴ 10 • written 24 months ago by
soheil
• 0
4
votes
9
replies
2.1k
views
6 follow
Same sequencing sample in multiple lanes. How to analyse it?
sequencing
bowtie2
alignment
samtools
ngs
updated 3 days ago by
ST
• 0 • written 2.2 years ago by
Federico
• 0
0
votes
1
reply
543
views
HOMER on AWS
HOMER
updated 3 days ago by
clairechung112
• 0 • written 2.2 years ago by
Bogdan
★ 1.4k
4
votes
9
replies
2.1k
views
Legend and hap files for imputation with 38 build
reference
38build
impute
imputation
6 days ago by
anna
▴ 20
16
votes
12
replies
7.4k
views
10 follow
how to split multi-fasta file into single fasta file named by header
genome
perl
python3
bash
python
updated 3 days ago by
rsieber
▴ 10 • written 3.1 years ago by
Kumar
▴ 120
2
votes
2
replies
1.8k
views
Parametric or non-parametric tests for qPCR significance testing between treatments?
qPCR
statistical test
parametric
non-parametric
updated 2 days ago by
e.r.zakiev
▴ 210 • written 3.8 years ago by
n.anuragsharma
▴ 40
3
votes
0
replies
1.1k
views
Tool:
Sars-Cov-2 Genome & ACE 2 Exploration
sequence
alignment
SNP
genome
1 day ago by
Ibrahim Tanyalcin
★ 1.2k
1
vote
14
replies
2.4k
views
Extract gRNA sequence using cutadapt
cutadapt
trimming
crispr
sequencing
updated 3 days ago by
GenoMax
142k • written 4.5 years ago by
Swimming bird
▴ 20
1
vote
11
replies
5.5k
views
Truncated Bam Error
R
next-gen
sequencing
software error
updated 14 hours ago by
guanghao
• 0 • written 6.1 years ago by
vivekruhela
▴ 20
40
votes
10
replies
42k
views
8 follow
Batch effects : ComBat or removebatcheffects (limma package) ?
limma
sva
Combat
batch-effect
updated 6 days ago by
cwwong13
▴ 40 • written 6.8 years ago by
lessismore
★ 1.3k
4
votes
6
replies
3.8k
views
How to interpret DEXseq results in therms of significance
DEXseq
RNA-Seq
exon
updated 4 hours ago by
Sara
▴ 30 • written 6.7 years ago by
Lila M
★ 1.2k
2
votes
2
replies
2.6k
views
Pindel Segmentation Fault Error
pindel
updated 2 days ago by
Ruqaiya
• 0 • written 10.2 years ago by
Yuelin
▴ 20
173 results • Page
4 of 4
Recent Votes
Comment: Gene Specific coverage from WGS data
Comment: Gene Specific coverage from WGS data
Comment: High Malat-1 expression in single cell data
A: Meme-Tools: Bit score
Meme-Tools: Bit score
Meme-Tools: Bit score
Comment: error in R code
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Recent Replies
Comment: anRichment is missing
by
Tatyana
• 0
Unfortunately, there are only tutorials and codes for tutorials for WGCNA, not installation files for any their packages... :(
Comment: anRichment is missing
by
GenoMax
142k
See this link for the WGCNA documentation and code from Peter Langfelder : https://bioinformatics.stackexchange.com/questions/21885/where-t…
Comment: anRichment is missing
by
Tatyana
• 0
The problem is back, Horwath's lab web is down again. Is it possible to get anRichment istallation file somewhere? (The greater problem is …
Comment: 16S rRNA microbiome differential abundance analysis with DESeq2 finds same micro
by
andres.firrincieli
3.6k
1. Maribaculaceae is a family not a genus 2. Who said that ASV with the same taxonomic classification must have the same behaviour? 3. With…
Comment: Question regarding WGCNA
by
andres.firrincieli
3.6k
the within-group variability is so small that these look like technical replicates rather than independent biological replicates
Answer: Gene ontology and homologs
by
geneontologyhelp
▴ 390
No. Homology is defined as shared common ancestry. Two genes that take part in the same biological process may not have the same functio…
Answer: error in R code
by
Matthias Zepper
4.6k
I quickly googled [the source code of that function][1], and the error is triggered by this condition: if(length(wn) < 1 || !is.whol…
Comment: Clarification regarding SAM flags "mate reverse strand" (flag 16/0x10) and "read
by
kalavattam
▴ 190
Thanks for the explanation. I noticed and corrected a typo in which "*read* reverse strand" initially read as "*mate* reverse strand" in t…
Comment: Spike-in control found in raw reads (16S amplicon seq) but not picked up by DADA
by
sovrappensiero
▴ 90
It's a commercial product, a *Staphylococcus hominis* genome. This species is not found in the DADA2 results, so I guess DADA2 is not ident…
Comment: Question regarding WGCNA
by
Ram
43k
Plots are incomplete without legends. Please share the complete plot with mappings for shape and color.
Comment: Quetion regarding WGCNA
by
deepak
• 0
Thank you for your reply andres.firrincieli. I have attached the PCA plot of the expression data used in the WGCNA. ![enter imag…
Comment: Design matrix Differential expression analysis
by
SHN
▴ 40
Got it, thank you for your response. SN
Comment: How to interpret DEXseq results in therms of significance
by
Sara
▴ 30
Hi, I used write.table(as.data.frame(DEXSeqResults(MyObj)), "Results.tsv", quote=FALSE, sep="\t", dec=".") to save the result but I recei…
Comment: Tissue-specific DEG analysis with DEseq2
by
M.
▴ 30
Can I ask what do you exactly mean by same experiment? If you are talking about the same donor and the same procedure, unfortunately, not a…
Comment: install package: package ‘gmwm’ is not available for this version of R
by
Ram
43k
Find someone with computer skills near you and consult them. From your posts on the forum, it's clear that you need help at a level that we…
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