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There are a lot of votes for Nextflow here, and it's generally pretty popular (and increasing in popularity). As a 'dissenting' voice, I'll…
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No, you can't do that with kallisto's bam files. For one, kallisto (by default) doesn't map to introns which is required by rMATs.
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It means that the gene is on the negative/minus strand. So you need to compute the reverse complementary sequence before translating it to …
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As long as the sample name matches across the lanes they are pieces of the same sample.
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Hi,
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With just one line example not sure if this will work
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Thank you! I found this as well and trying to understand it now.
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Thank you so much! This is where I am leading back to as well! I don't have much insights into Hisat2. I am going to give it a go this week…
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Interesting, why would you merge genes that are on different strands? They should be different genes. It cannot be done without modifying t…
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First, this is not bioinformatics. Simple pattern matching and extraction.
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