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9 days ago by
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Answer: Differential Expression using Isoseq-supplemented reference transcriptome
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> What is the best method to test for differential abundance at the isoform level? Salmon returns TPM values instead of the counts which Li…
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![enter image description here][1]
[1]: /media/images/aa60b9ef-b08c-4093-9f0d-fb656a5c
Comment: NGS forensics: how to know if data is fabricated
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benformatics
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Can you post an IGV screenshot of what this looks like?
Comment: NGS forensics: how to know if data is fabricated
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Yeah some of the Illumina machines pre-trim adapters (not sure if this is still the standard). I've also definitely also seen quality-trimm…
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You can typically find much more useful info in the cif file, for example what you've found is called '_atom_site.pdbx_PDB_ins_code ' and d…
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The best way to find out is to read the original paper. My guess is that the residue has been crystallized in alternative conformations, wh…
Answer: NGS forensics: how to know if data is fabricated
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I share the opinion by @philippbayer I have seen enough questionable things in life and in science to keep me permanently jaded, but there…
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I downloaded the data in two files from the link you provided. With this command:
megahit -1 ERR072246_1.fastq.gz -2 ERR072246_2.fastq…
Comment: How many reads for WGS Sequencing?
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If you have Linux the assembly should work on your system, but 8 GB is generally not enough for assembling larger genomes.
Comment: How many reads for WGS Sequencing?
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I think you might be getting stuck on less relevant parts of my exercise. The most important point was that nothing is wrong with the data.…
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Great comprehensive explanation and overview of the technologies -- with regards to sequencing assays, the one thing I'd add is SNV identif…
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I think that term means slightly different things in different contexts. For example, it's useful to profile mRNA modifications (e.g. m6a) …
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Hi, I met the same problem, have you found the solution?
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Hi @fe3e6f65 ,
In a single sentence, the answer to your question can be summarized as, "*the resolution of a genomic technology refers to …
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Without more context, we cannot interpret this PCA plot. There are many possibilities. In addition, this question is basic enough that it w…
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