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87 results • Page
2 of 2
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0
votes
0
replies
70
views
Why the shape of RCS is contradictory to the p value for nonlinearity.
RCS
ggplot2
ggplot
R
updated 17 hours ago by
GenoMax
142k • written 18 hours ago by
Dude
• 0
0
votes
1
reply
117
views
Polish a large genome with Pilon
Pilon
Pacbio
Polish
Illumina
16 hours ago by
sansan_96
▴ 90
0
votes
2
replies
160
views
Using Cutadapt to trim adapters from paired-end small RNA sequence data
trimming
adapters
cutadapt
sRNA-seq
smallRNA
12 hours ago by
melissa.joubert
• 0
0
votes
0
replies
56
views
ReactomeGSA
ReactomeGSA
12 hours ago by
Shaimaa Gamal
▴ 10
0
votes
3
replies
104
views
Problem with Calling Variants from RNA-Seq data
vcf
gatk
benchmark
giab
rna-seq
9 hours ago by
Esraa
• 0
0
votes
1
reply
94
views
bbmerge (bbmap) ~ error with insert size file output
bbtools
bbmerge
bbmap
updated 8 hours ago by
GenoMax
142k • written 14 hours ago by
chrisk
• 0
0
votes
1
reply
100
views
perseus software (version 1.5.5)
preseus
mass
spectrometry
data
updated 6 hours ago by
Joe
21k • written 16 hours ago by
iqra
• 0
0
votes
0
replies
48
views
Difference in number of reads when using duplex and simplex base calling with Dorado
base
calling
nanopore
simplex
dorado
duplex
6 hours ago by
Lélé
▴ 10
0
votes
0
replies
56
views
Seurat v5 and how to correctly integrate across multiple experiments
Seurat
scRNA
R
updated 5 hours ago by
ATpoint
82k • written 6 hours ago by
yura.grabovska
▴ 70
0
votes
0
replies
48
views
Is it okay to assemble my 12 metatranscriptome samples with Trinity separately?
trinity
metatranscriptome
4 hours ago by
jway
• 0
0
votes
0
replies
46
views
In one PCA plot, can I calculate the percentage of different factors that contribute to the PCA?
PCA
R
VARIANCE
4 hours ago by
diqixiaoyaoer
▴ 10
0
votes
1
reply
62
views
Wrong ZYGOSITY by ensembl vep on DeepVariant vcf file
annotation
vcf
vep
zygosity
deepvariant
4 hours ago by
asalimih
▴ 60
0
votes
1
reply
66
views
Can 5' and 3' scRNAseq be processed with the same pipeline?
singlecell
seurat
10XGenomics
scRNAseq
updated 3 hours ago by
yura.grabovska
▴ 70 • written 3 hours ago by
ev97
▴ 20
0
votes
2
replies
72
views
Blastn error : ncbi::CObject::ThrowNullPointerException() - Attempt to access NULL > pointer.
blast
meta
how-to
ncbi
forum
2 hours ago by
hophuquy0944
• 0
0
votes
0
replies
29
views
DOCK6 installation on MacOS M1
installation
troubleshooting
DOCK6
updated 2 hours ago by
GenoMax
142k • written 2 hours ago by
Rodolfo Adrián
• 0
0
votes
0
replies
15
views
Add line under stat test
stats
R
41 minutes ago by
Ghada
• 0
0
votes
0
replies
107
views
Pathway analysis of Bacteria from denovo genome assembly
KEGG-KASS
WGS
Pathway
denovo
updated 6 days ago by
Ram
43k • written 7 days ago by
mathavanbioinfo
▴ 90
0
votes
0
replies
11
views
What's next after GATK variant calling pipeline?
GATK
pipeline
figures
DNA-seq
20 minutes ago by
mgranada3
▴ 30
0
votes
0
replies
121
views
Annotate VCF via VEP for gnomAD annotation considering FILTER column
vep
vcf
gnomad
6 days ago by
asalimih
▴ 60
0
votes
0
replies
116
views
Summary Statistics SNPs not found in Target data
SNP
plink
PRS
updated 6 days ago by
Ram
43k • written 6 days ago by
curious_butterfly
• 0
0
votes
0
replies
179
views
Why does my Hi-C contact map show large regions making little to no contact?
BWA-MEM2
Hi-C
PretextMap
6 days ago by
Winter
• 0
0
votes
0
replies
106
views
how to downlad slices of CRAM fro ICGC ARGO
icgc
samtools
cram
6 days ago by
Javier
• 0
0
votes
0
replies
186
views
What is workflow for de-novo assembling of nuclear and mito genomes of non-model organisms
de-novo
WGS
DNA-seq
assembling
6 days ago by
Matvii Mykhailichenko
• 0
0
votes
1
reply
150
views
input file for alternative splicing in rmats in linux
rmats
updated 6 days ago by
Ram
43k • written 6 days ago by
Lambodarswain316
• 0
0
votes
0
replies
157
views
supervised admixture
supervised
admixture
5 days ago by
RT
▴ 10
0
votes
0
replies
152
views
Krona Pie-chart taxanomy IDs were not found warning. Taxonomy Classification Metagenomics
Classification
Metagenomics
Krona
Kraken2
5 days ago by
Aytaç
• 0
0
votes
0
replies
141
views
Allele specific expression of imprinted gens from 10x scRNA-seq data
scrna-seq
imprinted
10x
genes
5 days ago by
singcell
• 0
0
votes
0
replies
154
views
Trouble converting tfam and tped to map and ped files
plink
ped
tfam
tped
map
5 days ago by
Samantha
• 0
0
votes
2
replies
224
views
LncRNA Nomenclature
ENST
nomenclature
lncrna
GBB
ENSG
5 days ago by
jain72744
▴ 10
0
votes
1
reply
213
views
When should I use R-MarkDown over R-Script ?
r
rscript
rmarkdown
updated 5 days ago by
ATpoint
82k • written 5 days ago by
Amr
▴ 160
0
votes
1
reply
188
views
Microbial Signal Transduction Database
MiST
updated 5 days ago by
zx8754
11k • written 5 days ago by
Shravani
• 0
0
votes
0
replies
167
views
How do I calculate SE or P value if I only have BETA
prscsx
beta
se
pvalue
updated 5 days ago by
zx8754
11k • written 5 days ago by
curious_butterfly
• 0
0
votes
1
reply
184
views
Alignment of samples with spike-in
alignment
multimapping
RNA-seq
ChIP-seq
spike-in
updated 5 days ago by
GenoMax
142k • written 5 days ago by
maria.soler
• 0
0
votes
2
replies
268
views
fastq screen aligner no specified.
BWA
Bowtie2
FastqScreen
updated 5 days ago by
GenoMax
142k • written 12 days ago by
Ximena
• 0
0
votes
1
reply
217
views
How to convert Haps file to vcf file?
pre-phasing
GWAS
imputation
updated 5 days ago by
curious
▴ 750 • written 5 days ago by
SeoGyun
• 0
0
votes
2
replies
223
views
Presence of unknown sites in ANNOVAR output file
ANNOVAR
updated 4 days ago by
Ram
43k • written 6 days ago by
sainavyav22
• 0
0
votes
1
reply
199
views
extract viral protein of interest from 10k whole viral genomes
nBLAST
viral-genome
updated 4 days ago by
Ram
43k • written 5 days ago by
Shwetha
• 0
87 results • Page
2 of 2
Recent Votes
Answer: featureCounts output summary assigned value higher than uniquely mapped reads fr
Answer: Doubt about the process of annotation, detection, identification and classificat
Answer: Filtering VCF files based on VAF giving incorrect results
Problems with breakdancer (sv caller) output.
Problems with breakdancer (sv caller) output.
Comment: Add stats to boxplot in R
Answer: Genotyping sites with N in reference genome
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Recent Replies
Comment: Filtering VCF files based on VAF giving incorrect results
by
Pierre Lindenbaum
161k
> Do you think my commands were correct? I'm not familiar enough with this bcftools syntax. but what if there is no AD, what if both…
Answer: How can you *consistently* download BioProject IDs from NCBI's BioSample databas
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Ash
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I know this was asked several years ago, but finding this post helped me solve my own problem and in the process I solved this one too. …
Answer: DEG analysis with limma and contrast matrix using multiple Parkinson's cohorts i
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Would Parkinson's be expected to have an effect on blood cells? Neurons, sure, but blood cells?
Comment: Doubt about the process of annotation, detection, identification and classificat
by
MarcosCosta
• 0
Thank you very much, you've helped me a lot. Could you suggest a book or article that offers a complete explanation of these processes?
Comment: Filtering VCF files based on VAF giving incorrect results
by
Arton
• 0
Thank you. I can't try a completely new tool but I will keep your command for future use. Do you think my commands were correct? For exampl…
Comment: Add stats to boxplot in R
by
Ghada
• 0
Thanks. Thats work
Comment: Genotyping sites with N in reference genome
by
Zhenyu Zhang
★ 1.2k
Whether N -> A/T/G/C represents a variant is heavily dependent on the tool itself, and I know most tools don't. Since you don't see it in y…
Comment: calculating genomic coverage/ base overlap in R
by
marco.barr
▴ 110
The code seems correct at first glance... How did you generate the data in the Excel file? Have you checked the exon annotations? You could…
Comment: Blastn error : ncbi::CObject::ThrowNullPointerException() - Attempt to access NU
by
GenoMax
142k
Did you remeber to unzip/untar the `nt` preformatted files that you downloaded?
Comment: Genotyping sites with N in reference genome
by
shpak.max
▴ 50
Just to clarify, I have set additional sites in the reference genome to 'N', which means (based on your statement) that these will not be g…
Comment: Blastn error : ncbi::CObject::ThrowNullPointerException() - Attempt to access NU
by
Pierre Lindenbaum
161k
see https://www.biostars.org/p/9496674/ <!-- when i runed blastn(version 2.15) and nt database(latest version). I used aspera to download…
Answer: Genotyping sites with N in reference genome
by
Pierre Lindenbaum
161k
> Does UnifiedGenotyper skip/ignore sites where the reference genotype is N yes. The variant is in YOUR data, not in the reference where '…
Comment: Can 5' and 3' scRNAseq be processed with the same pipeline?
by
yura.grabovska
▴ 70
Do you have samples that should in theory have a good degree over overlap in terms of cell types across the two sequencing types? I would …
Comment: Wrong ZYGOSITY by ensembl vep on DeepVariant vcf file
by
asalimih
▴ 60
I found out that vep is only checking the GT field therefore assigning HOM to the Zygosity. Therefore the problem should be from deepvarian…
Comment: Add stats to boxplot in R
by
Lélé
▴ 10
To remove "Kruskall Wallis" you can try adding the argument label="p" or label = "p.signif" which displays the p-value or *** like this: …
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