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121,901 results • Page 27 of 2439
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3
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5
replies
1.3k
views
Tool for automatic immune cell annotation in MOUSE?
immune single-cell annotation
5 months ago by txema.heredia ▴ 250
4
votes
12
replies
7.8k
views
12 follow
How to draw venn diagram for two CNV lists?
venn
updated 6 months ago by 11k • written 10.8 years ago by ▴ 490
0
votes
1
reply
491
views
TMM normalized CPM
TPM CPM TMM
updated 6 months ago by 89k • written 6 months ago by ▴ 20
1
vote
0
replies
615
views
Normlizatiom in DiffBind
DiffBind CHIP-Seq
6 months ago by Siqi ▴ 10
1
vote
1
reply
563
views
Obtain genome coordinates for a DNA sequence using ENSEMBL API
Ensembl API
updated 6 months ago by 153k • written 6 months ago by • 0
0
votes
0
replies
861
views
Forum: Decoding Corvid Intelligence: Genomics, AI & the Genetics of Cognition
neurogenetics comparative-genomics bioinformatics genomics machine-learning
updated 6 months ago by 45k • written 6 months ago by • 0
3
votes
5
replies
1.0k
views
GBWT Construction Error: "Sources X and Y both have node Z" When Building GBZ from GFA
vg
updated 6 months ago by ▴ 760 • written 6 months ago by ▴ 20
8
votes
16
replies
1.8k
views
6 follow
prokka with too many "hypothetical proteins" and genome comparison
comparison genome galaxy prokka
6 months ago by avinci1 ▴ 20
0
votes
1
reply
633
views
Split paired ended hWGS FASTq files to simulate difference sequence coverage
whole-genome-sequencing wgs FASTQ coverage
updated 6 months ago by ★ 1.8k • written 6 months ago by ▴ 20
1
vote
11
replies
1.5k
views
OMA use case
oma omastandalone
updated 5 months ago by 153k • written 6 months ago by • 0
0
votes
0
replies
470
views
News: Machine Learning for Longitudinal Data with Python – Online Course (6-9 May)
MachineLearning LongitudinalData DeepLearning Omics
6 months ago by Physalia-courses ★ 2.6k
1
vote
2
replies
838
views
Recommended workflow to pre-process and demultiplex scRNAseq data based on the HTOs and ADTs
ADT HTO demultiplexing scRNAseq
updated 6 months ago by 153k • written 6 months ago by • 0
0
votes
3
replies
979
views
bwa-mem keeps failing to locate the index files
bwa bwa-mem
updated 6 months ago by 89k • written 6 months ago by ▴ 40
1
vote
5
replies
992
views
6 follow
Efficient Gene Prediction on Large Eukaryotic Genomes
tools prediction gene
updated 6 months ago by 22k • written 6 months ago by • 0
0
votes
0
replies
303
views
Olink - OlinkAnalyze Package - olink_umap_plot function
Olink proteomics
6 months ago by kristina • 0
3
votes
0
replies
19k
views
Herald: The Biostar Herald for Wednesday, March 19, 2025
herald
6 months ago by Biostar 3.6k
0
votes
1
reply
545
views
Cosmic database update issue in annovar
annovar cosmic genomics
updated 4 months ago by • 0 • written 6 months ago by • 0
1
vote
1
reply
645
views
Transforming diploid GT on male X and Y chromosome to haploid
VCF genotype gVCF
6 months ago by Michaela ▴ 10
3
votes
3
replies
903
views
How do I figure out pangenome location of hg38 coordinates?
pangenome hg38 vg
updated 5 months ago by • 0 • written 6 months ago by ▴ 10
5
votes
7
replies
1.4k
views
How I can curate all exon start-end positions for 50 genes (to create GTF file)?
gtf curation gene
updated 6 months ago by 153k • written 6 months ago by ▴ 10
0
votes
0
replies
353
views
generating manhattan plot through hail
plot manhattan hail visualizations gwas
6 months ago by nitya • 0
0
votes
0
replies
300
views
Hard Filtering Differences between WES and WGS
WES WGS Hard-Filtering
updated 6 months ago by 45k • written 6 months ago by • 0
0
votes
3
replies
638
views
Am I accounting for replicates correctly in pseudobulk analysis?
deseq2 single-cell pseudobulk
updated 6 months ago by 89k • written 6 months ago by ▴ 390
2
votes
4
replies
870
views
Is there too little variation to detect meaningful gene changes?
DESEQ
updated 6 months ago by 103k • written 6 months ago by ▴ 60
3
votes
5
replies
920
views
nanopore SNV callers returns empty VCF
snv_caller nanopore
updated 5 months ago by 103k • written 6 months ago by ▴ 10
15
votes
3
replies
2.6k
views
Tutorial: Avoiding the Pitfalls of the Anaconda License: A Practical Guide
conda Anaconda license
6 months ago by Juke34 9.3k
0
votes
2
replies
578
views
How many cells are needed in subcluster to draw conclusions?
immunology T-cells scRNA-seq
6 months ago by S • 0
0
votes
2
replies
599
views
Question about assigning gene names, tximport and DESeq2
tximport deseq2
5 months ago by bioinfo ▴ 160
3
votes
3
replies
853
views
RNASeq: Confused about adapters with barcodes and seq company's filtering
atria rnaseq adapter-trimming
updated 6 months ago by 153k • written 6 months ago by • 0
1
vote
2
replies
775
views
VEP won't show symbols for all variants; SnpEff will, but won't for others
vep snpeff
updated 6 months ago by 23k • written 6 months ago by ▴ 20
0
votes
2
replies
454
views
How to plot LMER estimate values in heatmap ?
cell scRNAseq TF lmer transcription
6 months ago by carolofharvest ▴ 50
1
vote
2
replies
1.7k
views
News: Final Call - Next-Generation Sequencing Data Analysis: A Practical Introduction (April 2-4 in Munich) - Only 2 seats left
Workshop RNAseq Variant-Calling Illumina DNAseq
6 months ago by ecSeq Bioinformatics ▴ 20
0
votes
1
reply
477
views
Guidance Needed: Best Practices for Handling Technical Replicates in RNA-seq Analysis
tehnical-replicates DESeq2 RNA-Seq
updated 6 months ago by 45k • written 6 months ago by • 0
0
votes
3
replies
821
views
Can anyone help me download the dbSNP data for Bos taurus ARS-UCD1.3?
GWAS genetics WGS
updated 6 months ago by 45k • written 6 months ago by • 0
0
votes
1
reply
493
views
Co-expression analysis of miRNAs and target mRNAs
miRNA targets co-expression miRNA-mRNA
updated 6 months ago by 22k • written 6 months ago by ▴ 60
0
votes
0
replies
299
views
Beagle Imputation
Beagle Imputation
6 months ago by mahlet.teka • 0
0
votes
0
replies
378
views
Issue with bam-readcount and fpfilter.pl: Saying "failed to get readcounts for variant allele"
bam-readount somaticsniper
updated 6 months ago by 166k • written 6 months ago by • 0
2
votes
2
replies
680
views
miRNA-seq: QC reports and workflow
miRNA-seq ION-Torrent RNA-seq FASTQC
6 months ago by omicon ▴ 40
1
vote
0
replies
272
views
How to Run Large-Scale Foldseek Comparisons (Human vs. C. elegans)
c.elegans Foldseek Genes Orthologs Homologs
6 months ago by bioinformatics_rk ▴ 10
2
votes
1
reply
993
views
Recommendations for Reference-guided de novo assembly assembly approaches or pipelines?
ont assembly wgs nanopore genomics
updated 6 months ago by ★ 1.8k • written 6 months ago by ▴ 60
0
votes
1
reply
466
views
Per Base Sequence Content FastQC
RNA-seq quality-control
updated 6 months ago by ★ 1.8k • written 6 months ago by ▴ 20
3
votes
2
replies
721
views
Seeking Advice on Handling Multiple Datasets for Differential Analysis in Transcriptomics
Data-Integration Differential-Analysis Transcriptomics Data-Normalization
6 months ago by Riley J • 0
2
votes
3
replies
668
views
Population genetics with mutect2 data
haplotypecaller mutect2
updated 6 months ago by 4.8k • written 6 months ago by ▴ 10
3
votes
2
replies
557
views
ggplot of two data set with different colors
geom_line scale_colour_manual ggplot2
updated 6 months ago by ▴ 20 • written 6 months ago by ▴ 80
1
vote
2
replies
587
views
Gene to use for phylogenic analysis
phylogenic-analysis
updated 6 months ago by 45k • written 6 months ago by • 0
0
votes
0
replies
315
views
Chrom3D
chrom3d interchromosomal genome model
6 months ago by desptsp • 0
0
votes
1
reply
471
views
Scanpy use with reticulate in R : var_group_positions argument set up
R Reticulate Scanpy
6 months ago by ZheFrench ▴ 590
1
vote
3
replies
648
views
CPTAC Data analysis
CPTAC proteomics
updated 6 months ago by 4.8k • written 6 months ago by ▴ 10
78
votes
13
replies
43k
views
10 follow
hg19 Centromere Position and Chromosome Length
centromere ucsc
updated 6 months ago by 45k • written 15.1 years ago by ▴ 260
0
votes
3
replies
730
views
How to Process Multiple SRRs for the Same BioSample ?
GEO RNA-seq NCBI miRNAs RNA
updated 6 months ago by 153k • written 6 months ago by ▴ 40
121,901 results • Page 27 of 2439
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Comment: STARlong for pacbio Isoseq reads
Comment: Automatically annotating a feature using a genbank file
Answer: Is this PCA plot correct??
Answer: Is this PCA plot correct??
Comment: Is this PCA plot correct??
Subsetting before QC in Spatial Transcriptomics
Comment: Subsetting before QC in Spatial Transcriptomics
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Comment: STARlong for pacbio Isoseq reads by Buffo ★ 2.4k
Thanks, I'll give it a try.
Comment: STARlong for pacbio Isoseq reads by GenoMax 153k
You could give `mapPacBio.sh` from BBMap suite a try while you wait for other answers. That works for reads up to 6 kb. BBMap has a new hom…
Comment: STARlong for pacbio Isoseq reads by Buffo ★ 2.4k
The average is 1.8kb. I was planning to post it on GitHub, but it seems like the repository has been unattended for the last months, so I d…
Comment: Getting just fastqc.zip file? by GenoMax 153k
Yes, You can either use it on command line or use the graphical user interface.
Comment: STARlong for pacbio Isoseq reads by GenoMax 153k
What is the average length of your reads? That error sounds like a bug, so you may want to post that on `STAR` repo as an issue. I assume …
Comment: Getting just fastqc.zip file? by pourhangleila95 • 0
I want to perform fastQC on my data.Do you use fastQC software for doing it?
Comment: Whole Genome Sequencing IG regions by 719 • 0
Sorry for the poor wording of the original post, also should say I really appreciate people taking a moment to look at my post. My main con…
Comment: Is total miRNA in EV constant? Implications for library size normalization. by Thomas.H.Hampton ▴ 10
Thanks again for your carefully considered reply. You asked what I am hoping to learn from a DE analysis. Autologous stem cells are well …
Comment: Subsetting before QC in Spatial Transcriptomics by Kent ▴ 30
Hi ATpoint, thank you very much for the helpful insight! I'm still doing some literature reading on this myself, but have you come across a…
Comment: Whole Genome Sequencing IG regions by ATpoint 89k
The question is what you are after. Is this a disease setup, or...well, what is this all about. Generally, these regions are hotspots of hy…
Comment: Whole Genome Sequencing IG regions by Pierre Lindenbaum 166k
please define "interesting"
Comment: Hifiasm is getting killed while trying to assemble a genome by pranavdatar01 • 0
These are PacBio HiFi reads (total reads 5,237,005), and the total data size is 255 GB. I am the only user on the system.
Comment: Subsetting before QC in Spatial Transcriptomics by ATpoint 89k
Yes, absolutely. It is even (to me) a good practice to do (automated) crude celltype assignment first because QC metrics, such as number of…
Comment: Hifiasm is getting killed while trying to assemble a genome by pranavdatar01 • 0
I am expecting the genome to be around 1.5 Gb.
Comment: Hifiasm is getting killed while trying to assemble a genome by GenoMax 153k
> I even tried to decrease the number of threads and even used -f0, but nothing is working out. What kind of dataset is this? How many r…
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