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121,901 results • Page 28 of 2439
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3
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774
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Methylation cpg probes annotation
annotation methylation cpg TCGA
updated 6 months ago by 153k • written 6 months ago by ▴ 10
1
vote
2
replies
779
views
How to convert .ab1 to fastq
Fastq
updated 6 months ago by ▴ 120 • written 6 months ago by • 0
0
votes
1
reply
418
views
How to deal with missing annotations in RNASeq data?
eggnogmapper RNASeq
updated 6 months ago by ★ 3.1k • written 6 months ago by ▴ 140
0
votes
2
replies
551
views
Why protein sequence from AUGUSTUS can't find in blast
genome AUGUSTUS galaxy annotation
6 months ago by Jl • 0
1
vote
2
replies
599
views
How to make amazing UMAP to spatialPlot GIF
umap spatial
6 months ago by noodlejackson ▴ 40
1
vote
1
reply
432
views
Software for visualizing mass spectromety spectra
MS Mass Spectrometry
updated 6 months ago by 22k • written 6 months ago by ▴ 40
1
vote
1
reply
368
views
Detecting Aneuploidy from Bulk RNA-Seq Data
Aneuploidy RNA-Seq
updated 6 months ago by 89k • written 6 months ago by ▴ 10
0
votes
0
replies
382
views
GSA genome studio call rate calculation issues
Genome GSA studio
6 months ago by 1769mkc ★ 1.3k
1
vote
2
replies
853
views
Parsing BAM file per cell from Smart-Seq2 dataset
starsolo star BAM smartseq2 alignment
updated 6 months ago by ★ 7.7k • written 6 months ago by • 0
0
votes
0
replies
473
views
News: Spring School in Bioinformatics - online, March 31 to April 4
Singularity NGS RNAseq Phylogenomics Docker
6 months ago by Physalia-courses ★ 2.6k
2
votes
2
replies
734
views
Gene Expression Timeline Analysis for N=1
Analysis Gene Expression
6 months ago by ATRX ★ 1.2k
0
votes
1
reply
881
views
Interactive UMAP for scRNA-seq Data
HTML RMarkdown Interactive-UMAP scRNA-seq
updated 6 months ago by • 0 • written 7 months ago by • 0
0
votes
0
replies
332
views
Converting PLINK GWAS Summary Statistics (--glm) to BED Format for UCSC Liftover
PLINK LiftOver PLINK2 BED GWAS
6 months ago by Mllepnos • 0
3
votes
0
replies
462
views
Tool: PAN-GO Functionome: known functions of all human protein-coding genes
goenrichment function GO goterms geneontology
6 months ago by geneontologyhelp ▴ 470
3
votes
1
reply
577
views
How to clarify results from an HLA association analysis - Omnibus tests/HATK/other options
Omnibus HLA HATK GWAS Regression
updated 6 months ago by ▴ 30 • written 6 months ago by ▴ 60
0
votes
0
replies
454
views
News: Multi-Omics Integration with Machine Learning — Online Course!
Machine-Learning Multi-Omics Depp-Learning Data-Integration BigData
6 months ago by Physalia-courses ★ 2.6k
3
votes
2
replies
636
views
Alignment algorithm for DNA sequencing
sequencing data DNA
updated 6 months ago by 89k • written 6 months ago by ▴ 80
0
votes
1
reply
609
views
RepeatMasker UCSC track
RepeatMasker
updated 6 months ago by 56k • written 6 months ago by • 0
0
votes
0
replies
366
views
rarefaction curve metagenomic reads show species saturation around 100 000 reads
metagenomics rarefaction kraken2
6 months ago by rDNA ▴ 20
0
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4
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1.3k
views
Help with CIBERSORTx Error in Single-Cell RNA-seq Analysis: Invalid 'times' Argument
cibersortx
updated 6 months ago by ★ 19k • written 6 months ago by • 0
10
votes
22
replies
15k
views
13 follow
BLAST Database error: Database memory map file error
makeblastdb
updated 23 days ago by ▴ 30 • written 12 months ago by • 0
0
votes
2
replies
548
views
Looking up gene name from mRNA ID using biomaRt
R mRNA biomaRt
updated 6 months ago by 45k • written 6 months ago by • 0
0
votes
0
replies
325
views
Interpreting negative gap length in MUMMER4
mummer translocation
6 months ago by cedric.blais ▴ 20
0
votes
0
replies
319
views
Uncertainty about deepTools computeMatrix options for RNA modifications
deepTools metagene computeMatrix
6 months ago by Kyle • 0
0
votes
0
replies
412
views
News: Live Online Course: Introduction to Python, April, 2025
python programming course
6 months ago by soledad.esteban • 0
6
votes
6
replies
1.1k
views
Multiple factors in DESeq2
deseq2
6 months ago by noodlejackson ▴ 40
10
votes
2
replies
673
views
Why do RNAseq reads align to non-gene regions of the bacterial genome?
RNAseq transcription
updated 6 months ago by 22k • written 6 months ago by ▴ 40
1
vote
1
reply
713
views
Confused about discovering novel genes in a model organism using STAR, Trinity, and reference genomes
star trinity rnaseq
updated 6 months ago by 45k • written 6 months ago by ★ 4.4k
5
votes
7
replies
1.0k
views
nanopore variant calling
pacBio long-read nanopore
updated 6 months ago by 7.9k • written 6 months ago by ▴ 10
0
votes
3
replies
758
views
enrichGo problem
enrichGo
updated 6 months ago by 3.4k • written 6 months ago by • 0
1
vote
4
replies
791
views
Seeking Help for Reanalysis of Raw WES Data
WES
updated 6 months ago by 23k • written 6 months ago by • 0
1
vote
8
replies
1.0k
views
Biscuit Alignment and Index failed - WGBS - Bisulfite analysis
BWA Biscuit Bisulfite
updated 6 months ago by 45k • written 6 months ago by • 0
2
votes
3
replies
691
views
illumina: Adapter Confusion
ILLUMINA ADAPTER
updated 6 months ago by 153k • written 6 months ago by ▴ 20
2
votes
5
replies
933
views
Identifying primer sequences from raw FASTQ files
primers
5 months ago by Chandini • 0
2
votes
7
replies
1.9k
views
HTSlib threads when ingesting large bgzipped FASTA
htslib samtools bgzip c
updated 6 months ago by ▴ 390 • written 2.2 years ago by • 0
3
votes
3
replies
767
views
question for dowloading Protein fasta sequence by edirect using while
edirect
updated 6 months ago by ★ 3.9k • written 6 months ago by • 0
2
votes
2
replies
1.1k
views
Validate RNAseq Models in Microarray Data?
microarray rnaseq coxlasso fpkm
updated 6 months ago by 89k • written 3.2 years ago by ▴ 130
0
votes
0
replies
336
views
Restrict anlaysis of WGS VCF file to Refseq Select using snpeff
refseq vcf wgs
6 months ago by Gabriel • 0
0
votes
2
replies
637
views
Novel genes in model organisms
homologue genome gene
6 months ago by zizigolu ★ 4.4k
0
votes
1
reply
596
views
Understanding sctransform
sctransform Seurat
updated 6 months ago by 6.5k • written 6 months ago by ★ 4.2k
3
votes
1
reply
974
views
Subset reads based on Phred-score ranges
phred sequencing ngs
updated 6 months ago by 153k • written 6 months ago by ▴ 60
6
votes
3
replies
892
views
Reasoning - Why is it invalid to compare ratios of TPMs?
RNAseq analysis expression normalization differential TPM batch effect
updated 6 months ago by 89k • written 6 months ago by • 0
2
votes
2
replies
895
views
DEGs Analysis For a Microarray Data that has one biological replicates per cell line in each condition
Analysis DEGs Microarray
6 months ago by mete.han.celebi • 0
3
votes
6
replies
1.0k
views
The soapdenovo2 genome assembly is highly fragmented - learning genome assembly with public data
Gene soapdenovo2 assembly
updated 6 months ago by 15k • written 6 months ago by • 0
6
votes
3
replies
1.2k
views
VCF filtering with Allele Balance (AB) using FilterVcf (Picard)
Allele-Balance FilterVcf Picard GATK
updated 6 months ago by ▴ 170 • written 6 months ago by ▴ 20
1
vote
4
replies
1.1k
views
Haplotype analysis
founder haplotype-analysis
6 months ago by disha3786 • 0
3
votes
4
replies
860
views
Indica rice genome and annotation files
RNASeq
updated 6 months ago by ▴ 10 • written 6 months ago by • 0
2
votes
6
replies
1.1k
views
Question: Making primers for cloning and transfection
transfection cloning primer PCR gene
updated 6 months ago by ★ 19k • written 6 months ago by • 0
0
votes
0
replies
365
views
Help with the interpretation of Silhouette width averaging
clustering kmeans cluster silhouettemethod
6 months ago by Maria Jose • 0
0
votes
1
reply
1.0k
views
Job: Open Rank Tenure-earning Faculty in the Department of Biostatistics & Bioinformatics
Cancer Oncology Tenure-earning Faculty Bioinformatics
updated 6 months ago by 153k • written 6 months ago by • 0
121,901 results • Page 28 of 2439
Recent Votes
Shift in RNA 3' position, Term-Seq vs RNASeq
Comment: STARlong for pacbio Isoseq reads
Comment: Automatically annotating a feature using a genbank file
Answer: Is this PCA plot correct??
Answer: Is this PCA plot correct??
Comment: Is this PCA plot correct??
Subsetting before QC in Spatial Transcriptomics
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Recent Replies
Comment: STARlong for pacbio Isoseq reads by Buffo ★ 2.4k
Thanks, I'll give it a try.
Comment: STARlong for pacbio Isoseq reads by GenoMax 153k
You could give `mapPacBio.sh` from BBMap suite a try while you wait for other answers. That works for reads up to 6 kb. BBMap has a new hom…
Comment: STARlong for pacbio Isoseq reads by Buffo ★ 2.4k
The average is 1.8kb. I was planning to post it on GitHub, but it seems like the repository has been unattended for the last months, so I d…
Comment: Getting just fastqc.zip file? by GenoMax 153k
Yes, You can either use it on command line or use the graphical user interface.
Comment: STARlong for pacbio Isoseq reads by GenoMax 153k
What is the average length of your reads? That error sounds like a bug, so you may want to post that on `STAR` repo as an issue. I assume …
Comment: Getting just fastqc.zip file? by pourhangleila95 • 0
I want to perform fastQC on my data.Do you use fastQC software for doing it?
Comment: Whole Genome Sequencing IG regions by 719 • 0
Sorry for the poor wording of the original post, also should say I really appreciate people taking a moment to look at my post. My main con…
Comment: Is total miRNA in EV constant? Implications for library size normalization. by Thomas.H.Hampton ▴ 10
Thanks again for your carefully considered reply. You asked what I am hoping to learn from a DE analysis. Autologous stem cells are well …
Comment: Subsetting before QC in Spatial Transcriptomics by Kent ▴ 30
Hi ATpoint, thank you very much for the helpful insight! I'm still doing some literature reading on this myself, but have you come across a…
Comment: Whole Genome Sequencing IG regions by ATpoint 89k
The question is what you are after. Is this a disease setup, or...well, what is this all about. Generally, these regions are hotspots of hy…
Comment: Whole Genome Sequencing IG regions by Pierre Lindenbaum 166k
please define "interesting"
Comment: Hifiasm is getting killed while trying to assemble a genome by pranavdatar01 • 0
These are PacBio HiFi reads (total reads 5,237,005), and the total data size is 255 GB. I am the only user on the system.
Comment: Subsetting before QC in Spatial Transcriptomics by ATpoint 89k
Yes, absolutely. It is even (to me) a good practice to do (automated) crude celltype assignment first because QC metrics, such as number of…
Comment: Hifiasm is getting killed while trying to assemble a genome by pranavdatar01 • 0
I am expecting the genome to be around 1.5 Gb.
Comment: Hifiasm is getting killed while trying to assemble a genome by GenoMax 153k
> I even tried to decrease the number of threads and even used -f0, but nothing is working out. What kind of dataset is this? How many r…
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