Latest
Open
Jobs
Tutorials
Tags
About
FAQ
Community
Planet
New Post
Log In
New Post
Latest
Open
News
Jobs
Tutorials
Forum
Tags
Planet
Users
Log In
Sign Up
About
Limit : all time
all time
today
this week
this month
this year
121,196 results • Page
6 of 2424
Sort: Rank
Rank
Views
Votes
Replies
1
vote
3
replies
538
views
Visualise duplications and inversions
inversions
duplications
updated 4 weeks ago by
cmdcolin
★ 4.2k • written 4 weeks ago by
priya.bmg
▴ 70
1
vote
3
replies
522
views
Exon-level counts via featureCounts
diffSpliceDGE
exon
RNASeq
featureCounts
edgeR
updated 28 days ago by
rfran010
★ 1.5k • written 4 weeks ago by
n_navy
▴ 10
0
votes
5
replies
593
views
Picard : Bait_interval and Target_interval
Picard
Bed
read-depth
coverage
updated 4 weeks ago by
Ram
45k • written 4 weeks ago by
AIMAR
▴ 10
3
votes
2
replies
460
views
Does vg giraffe output secondary and supplimentary alignments?
vg
giraffe
4 weeks ago by
lushjia
• 0
0
votes
0
replies
344
views
Question about Calculation of Dietary index in the NHANES Database
index
DI-GM
Dietary
NHANES
4 weeks ago by
城玮
• 0
1
vote
1
reply
460
views
Detection SNPs and Indels Bacteria
SNPs
updated 4 weeks ago by
Istvan Albert
102k • written 4 weeks ago by
Eltercertopico
• 0
1
vote
4
replies
658
views
protease candidates for my protein of interest
cleaving-site
proteases
protein
4 weeks ago by
Fatemeh
▴ 40
1
vote
3
replies
522
views
Different number of fragments after doublets removal in each run
ArchR
4 weeks ago by
Bioinformatics_16
• 0
1
vote
2
replies
455
views
Seeking Guidance on Next Steps for DNA Methylation Biomarker Screening in Cancer Prognosis
early-cancer-screening
TCGA
Epigenomics
DNA-Methylation
cancer-prognosis
4 weeks ago by
Riley J
• 0
0
votes
1
reply
438
views
Help with Panel of Normal (PON)
somatic-mutation
PoN
updated 4 weeks ago by
Ram
45k • written 4 weeks ago by
TJ
• 0
9
votes
5
replies
827
views
6 follow
Why don't I see people calculating FPKM from normalized read counts?
normalization
RNA-seq
updated 29 days ago by
kalavattam
▴ 350 • written 4 weeks ago by
shelkmike
★ 1.6k
1
vote
9
replies
1.0k
views
Error during running wf-bacterial-genome (nextflow run epi2me-labs/wf-bacterial-genomes).
EPI2ME
MEDAKA
updated 4 weeks ago by
GenoMax
151k • written 4 weeks ago by
Anurag
• 0
0
votes
0
replies
328
views
freebayes with pools of different number of individuals
freebayes
poolseq
4 weeks ago by
Jeanlain
• 0
0
votes
3
replies
498
views
Insight into Trimmomatic
Trimmomatic
Trimming
Reads
updated 4 weeks ago by
GenoMax
151k • written 4 weeks ago by
jh5ym
• 0
1
vote
3
replies
552
views
Trouble when uploading FASTQ files on NCBI
rnaseq
ncbi
4 weeks ago by
Gerard
▴ 20
1
vote
4
replies
596
views
SyntaxError in file /snakefile, line 22: invalid decimal literal: None
python
snakemake
updated 4 weeks ago by
Jesse
▴ 870 • written 4 weeks ago by
Ana
▴ 10
3
votes
2
replies
578
views
News:
Building an mmCIF parser for enzyme structure analysis — feedback & ideas welcome!
mmcif
4 weeks ago by
Shravya
• 0
0
votes
0
replies
368
views
News:
Course: Introduction to Genome Annotation (Online, 19–22 May) - last call
Gene-Prediction
Genome-Annotation
Gene-Models
14 days ago by
Physalia-courses
★ 2.6k
0
votes
1
reply
411
views
Error with running cellranger-atac count
cellranger
atac
single-cell
updated 4 weeks ago by
Ram
45k • written 4 weeks ago by
Khôi
• 0
0
votes
2
replies
499
views
HIC-pro with Forward and reverse reads not paired even when I use their test data.
HIC-pro
4 weeks ago by
Aki
▴ 20
1
vote
1
reply
549
views
Using DEXseq on kallisto tsv outputs ?
alignement
DEXseq
kallisto
updated 5 weeks ago by
Gordon Smyth
★ 8.1k • written 5 weeks ago by
Gerard
▴ 20
0
votes
0
replies
450
views
Job:
2025 Arrowhead AI/LLM for Computational Biology and Data Science Internship
intern
updated 5 weeks ago by
GenoMax
151k • written 5 weeks ago by
Shicheng Guo
★ 9.6k
1
vote
2
replies
766
views
ChIP-seq gene blacklist for Rattus norvegicus
rat
blacklist
ChIP-seq
updated 5 weeks ago by
GenoMax
151k • written 13 months ago by
Thomas
• 0
4
votes
10
replies
1.3k
views
6 follow
Extract length of sample sequence from vcf
microsatellites
bcftools
vcf
indels
variants
updated 5 weeks ago by
cmdcolin
★ 4.2k • written 6 weeks ago by
jahnreinhard.ringger
• 0
0
votes
0
replies
387
views
Tutorial:
Fast SCENIC GRN Analysis with RegDiffusion
GRN
network
scRNA-seq
wgcna
5 weeks ago by
Hao
▴ 10
2
votes
3
replies
922
views
Is it reasonable to do "Gene Regulatory Network Inference" for each cell type and do differential GRN
GRN
scRNA-seq
updated 5 weeks ago by
Hao
▴ 10 • written 2.6 years ago by
shangguandong1996
▴ 30
1
vote
2
replies
637
views
Blastx issue
blastx
lncRNA
updated 4 weeks ago by
Ram
45k • written 5 weeks ago by
Avik
• 0
2
votes
9
replies
971
views
TPM vs FPKM divergence at high values
tpm
fpkm
normalisation
updated 4 weeks ago by
Istvan Albert
102k • written 5 weeks ago by
megancgrdnr
• 0
3
votes
0
replies
358
views
Herald:
The Biostar Herald for Monday, April 14, 2025
herald
5 weeks ago by
Biostar
3.5k
1
vote
5
replies
696
views
What is the best way to know if a pair of genes is random?
go-terms
genes
25 days ago by
Ana
▴ 10
1
vote
1
reply
481
views
How to save the AlphaFold PAE plot as an image in ChimeraX using command line ?
PAE
ChimeraX
AlphaFold
updated 5 weeks ago by
LauferVA
4.7k • written 5 weeks ago by
Picasa
▴ 680
1
vote
0
replies
286
views
News:
LAST CALL - Bioinformatics Pipeline Development with Nextflow - May 19-21, 2025 (online) - Apply Now
nextflow
rnaseq
workshop
5 weeks ago by
ecSeq Bioinformatics
▴ 20
1
vote
2
replies
459
views
RNA-seq, three comparisons - stick to one linear model, or use separate models?
statistics
mixed-effect-model
stats
RNAseq
linear-model
updated 4 weeks ago by
Ram
45k • written 5 weeks ago by
noodlejackson
▴ 40
1
vote
1
reply
343
views
Using Principal Components may muddle the detecting power of a GWAS?
q-value
covariates
genomic-inflation
pca
gwas
updated 5 weeks ago by
DBScan
▴ 480 • written 5 weeks ago by
AndrMod
• 0
0
votes
0
replies
242
views
Use Seed or Full Alignments from Pfam HMM as a filter step
hmm
sequences
metagenomic
5 weeks ago by
Jane123
• 0
3
votes
4
replies
515
views
Helicobacter pylori low-rate alignment
RNA-seq
transcriptomics
updated 4 weeks ago by
Ram
45k • written 5 weeks ago by
Ariadna
▴ 20
14
votes
3
replies
11k
views
Can The Wget Command Be Used To Download Pdbs
pdb
updated 5 weeks ago by
Calvin
• 0 • written 13.6 years ago by
Harpalss
▴ 310
1
vote
3
replies
538
views
Is a log2 transformation an essential step in preparing expression data for machine learning?
Machine-learning
preproccessing
microarray
updated 4 weeks ago by
Ram
45k • written 5 weeks ago by
Sib
▴ 70
0
votes
2
replies
1.0k
views
How to re-annotate the structure of gene against a known genomic file
RNA-Seq
updated 5 weeks ago by
Mensur Dlakic
★ 29k • written 6.0 years ago by
Kanoo
• 0
0
votes
2
replies
409
views
ONT Live-Basecalling Issue?
ONT
minKNOW
live-basecalling
updated 4 weeks ago by
LauferVA
4.7k • written 5 weeks ago by
Akshay
• 0
3
votes
4
replies
482
views
Identify gene from sequence at scale in non-model organisms
sequence
gene
updated 4 weeks ago by
Ram
45k • written 5 weeks ago by
matthewmerkin32
• 0
0
votes
0
replies
253
views
News:
Cellosaurus release 52 is available
biocuration
database
knowledgebase
cell-line
updated 4 weeks ago by
Ram
45k • written 5 weeks ago by
Amos Bairoch
▴ 130
0
votes
2
replies
462
views
WGCNA
WGCNA
updated 4 weeks ago by
Ram
45k • written 5 weeks ago by
香
• 0
0
votes
0
replies
542
views
Seeking Advice on Causal Inference for Treatment Effect Prediction (Small Sample, Genomic Covariates)
causal-inference
updated 4 weeks ago by
Ram
45k • written 5 weeks ago by
oghzzang
▴ 50
5
votes
5
replies
659
views
Batch effect
RNAseq
Batch-Effect
5 weeks ago by
Fatemeh
▴ 40
0
votes
4
replies
597
views
convert Gene ID to Gene Symbol
gene-id
updated 5 weeks ago by
pinheirofabiano
▴ 110 • written 5 weeks ago by
majorprogeria
• 0
0
votes
1
reply
333
views
News:
Structural bioinformatics workshop
Structural-bioinformatics
updated 4 weeks ago by
Ram
45k • written 5 weeks ago by
reetesh
• 0
1
vote
10
replies
893
views
How to test the similarity of two gene expression profiles
gene-sets
updated 4 weeks ago by
Ram
45k • written 5 weeks ago by
htchd
• 0
3
votes
3
replies
421
views
Which are the different kinds of GeneID that exist?
R
RNA-Seq
5 weeks ago by
pinheirofabiano
▴ 110
0
votes
0
replies
253
views
Error using DWLS
deconvolution
DWLS
RNAseq
5 weeks ago by
Ana
▴ 10
121,196 results • Page
6 of 2424
Recent Votes
Comment: How important are allele fractions when calling variants?
Answer: DESeq2 outliers-should I be concerned?
A: fasta to fastq without quality scores
C: fasta to fastq without quality scores
Answer: Cross-species differential gene expression, finding orthologs
C: FRIP score ATAC-seq
Answer: Trimmomatic command
Recent Locations •
All
UCLA,
4 minutes ago
Machhagan,
8 minutes ago
India,
11 minutes ago
United Kingdom,
31 minutes ago
United States,
46 minutes ago
Bangladesh,
59 minutes ago
Kazakhstan,
1 hour ago
Recent Awards •
All
Teacher
to
cfos4698
★ 1.1k
Popular Question
to
wiscoyogi
▴ 40
Popular Question
to
curious
▴ 860
Popular Question
to
aaaaaaa
• 0
Popular Question
to
reza
▴ 300
Popular Question
to
Madelaine Gogol
5.3k
Popular Question
to
David Langenberger
11k
Recent Replies
Comment: How common is it to split fastq files prior to bwa mem to increase parallelizati
by
dsull
★ 7.5k
Btw, for splitting, in my hands, piping `unpigz` into the unix `split` command seemed to work faster than `seqkit`.
Answer: Download GenomicSEM reference file
by
zhangmyharper
• 0
Sorry for that,I have found it.
Answer: How common is it to split fastq files prior to bwa mem to increase parallelizati
by
dsull
★ 7.5k
Splitting is a good strategy for speeding up processing. I use it all the time in my Snakemake workflows. Processing multiple files in para…
Comment: DESeq2 outliers-should I be concerned?
by
bioinfo
▴ 150
Thank you so much for the explanation!
Comment: How common is it to split fastq files prior to bwa mem to increase parallelizati
by
ATpoint
88k
4GB is in no way enough. The sort alone uses 768MB RAM per thread, so 8*768 already exceeds the memory. bwa with that many threads will use…
Comment: How common is it to split fastq files prior to bwa mem to increase parallelizati
by
curious
▴ 860
This run is running on a node with 60 cores but I only requested 4GB of RAM. Seem like it is only using 2 CPUs to near 100% if I open htop.…
Comment: How common is it to split fastq files prior to bwa mem to increase parallelizati
by
ATpoint
88k
What is your hardware and command line? Two days sounds excessive, unless you are limited by RAM and cores, and that then would make it que…
Comment: Difference in mapping rate when aligned with mapper.pl of miReep2 and bowtie1
by
LINHUI
• 0
Thanks! It worked for me
Comment: Cross-species differential gene expression, finding orthologs
by
fe94
▴ 10
I'm doing a genome-guided assembly (using publicly available genomes info) of the transcripts, we generated RNAseq data for all the species…
Comment: Cross-species differential gene expression, finding orthologs
by
GenoMax
151k
> I'm using genome and gtf info that are publicly available to map and assemble the transcripts for all 3 species. Are you assembling the …
Answer: Longest Protein per Gene from gpff file
by
lieven.sterck
15k
Have a look ar [AGAT][1] , with special focus on the `agat_sp_keep_longest_isoform.pl` subcommand. You might first need to convert the gen…
Comment: Cross-species differential gene expression, finding orthologs
by
fe94
▴ 10
Thank you for the suggestion!! Yes cross-species DEG analysis, especially for non-model species, seems pretty tricky still.
Comment: Cross-species differential gene expression, finding orthologs
by
fe94
▴ 10
Hi GenoMax, I'm not sure I got your question right. I'm using genome and gtf info that are publicly available to map and assemble the trans…
Comment: PRS result interpretation
by
Sam
★ 4.8k
Currently, we still haven't figure out how to best use PRS on individual level. What are your research question? Why do you need to use a P…
Comment: How important are allele fractions when calling variants?
by
Jeremy Leipzig
23k
The allele fraction is the main thing but only after artefactual alignments have been removed. If the AF is .2 but the other .8 of reads sh…
Traffic: 7879 users visited in the last hour
Content
Search
Users
Tags
Badges
Help
About
FAQ
Access
RSS
API
Stats
Use of this site constitutes acceptance of our
User Agreement and Privacy Policy
.
Powered by the
version 2.3.6