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1,000 results • Page 1 of 20
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7
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I need help with a methyl array data analysis
methyl_array DMPs DMRs beta_value r
updated just now by ▴ 650 • written 15 minutes ago by ▴ 10
0
votes
0
replies
16
views
Error in download library file in expression console software
expression microarray console
1 hour ago by Mohammad • 0
1
vote
2
replies
65
views
ScRNA data
Data ScRNA
updated 2 hours ago by ▴ 20 • written 3 hours ago by • 0
1
vote
2
replies
69
views
extract all fasta sequence from db v5
blast protein
updated 2 hours ago by 129k • written 4 hours ago by ▴ 80
743
votes
162
replies
113k
views
101 follow
News: The Biostar Handbook. A bioinformatics e-book for beginners.
training handbook
updated 4 weeks ago by 1.8k • written 6.5 years ago by 98k
3
votes
2
replies
84
views
Does the RNAseq data normal if the TPM value 3rd Qutile expression is near 10, but the Max expression are near 20,000
Expression RNAseq
2 hours ago by alwayshope ▴ 20
0
votes
4
replies
135
views
Demultiplexing bam file
demultiplexing bam_file Iontorrent
updated 2 hours ago by 129k • written 8 hours ago by ▴ 60
1
vote
2
replies
127
views
DEgs RNAseq
RNAseq
2 hours ago by Juan • 0
1
vote
3
replies
309
views
Counting intronic reads in bulk RNA-seq
rna-seq
updated 11 hours ago by ★ 4.2k • written 1 day ago by ▴ 300
0
votes
4
replies
230
views
Single cell chemistry
cell single chemistry
40 minutes ago by David • 0
0
votes
0
replies
83
views
Different alternatives for downloading GWAS summary statistics en masse
GWAS download data
17 hours ago by Vincent Laufer ★ 2.8k
0
votes
6
replies
362
views
Why are some WES VCFs larger than others?
WES sequencing genomics NGS exome
updated 21 hours ago by ★ 2.8k • written 1 day ago by ▴ 80
0
votes
4
replies
278
views
SyntaxError (Perhaps you forgot a comma?) in snakefile when running FastQC.
snakemake
19 hours ago by Dhatri Badri • 0
0
votes
0
replies
101
views
problem of Global Biobank Meta-analysis Initiative
GWAS
1 day ago by 1362321710 • 0
0
votes
1
reply
141
views
DownsampleSam
picard DownsampleSam
updated 23 hours ago by 154k • written 1 day ago by • 0
0
votes
2
replies
214
views
ABySS genome assembler
ABySS genome k-mers assembly
1 day ago by npavliukovec • 0
0
votes
1
reply
147
views
Error while running Megahit
Megahit
updated 11 hours ago by ★ 23k • written 1 day ago by • 0
0
votes
1
reply
168
views
How to deal with duplicated gene IDs in TCGA RNA expression data?
TCGA Expression mRNA
updated 17 hours ago by ★ 2.8k • written 1 day ago by ▴ 30
1
vote
5
replies
287
views
Cutadapt error: too many parameters.
cutadapt parallel bash
updated 1 day ago by 129k • written 2 days ago by • 0
0
votes
0
replies
133
views
corresponding clinical data from TCGA database of PSI values for CCA AS events in spliceseq database
TCGA
2 days ago by dima • 0
1
vote
0
replies
186
views
How to assess which cell population is most affected by treatmant in single cell rna seq data?
transcriptomics single-cell RNA DEG
2 days ago by abbas89 ▴ 10
0
votes
3
replies
310
views
metric informing about the similarity of gene lists in OrderedList
orderedlist
updated 2 days ago by ★ 2.8k • written 3 days ago by • 0
18
votes
6
replies
6.4k
views
VCF header line counting
vcf header
updated 2 days ago by ▴ 530 • written 8.3 years ago by ▴ 40
0
votes
1
reply
159
views
How extract specific SNP position from the bed file-Bedtools
bedtools for Help
updated 1 day ago by 129k • written 2 days ago by • 0
0
votes
0
replies
453
views
Can I send my PyMol results to a web-page as a result for a submitted query? If not, how can I get a 3d render of a protein as a result for a user su…
protein-structure pymol protein pdb python
2 days ago by Aatif Hashmi • 0
2
votes
5
replies
391
views
Design matrix to remove technical replicate effect
edgeR
2 days ago by ARD ▴ 10
0
votes
1
reply
237
views
Deferentially expressed gene with high log2foldchange by DESeq2; but not meaningful at the individual level
DESeq2 RNA-seq DGEA
updated 2 days ago by ★ 2.8k • written 2 days ago by ▴ 10
1
vote
2
replies
186
views
Abyss taking a Long time to run
abyss assembly genome fastq
2 days ago by Maliha ▴ 20
0
votes
2
replies
211
views
Import or read Snapgene .dna files into R
R .dna snapgene
2 days ago by eggrandio ▴ 40
2
votes
2
replies
207
views
Segmentation fault Biopython pairwise alignment
biopython alignment
updated 2 days ago by 98k • written 2 days ago by ▴ 20
0
votes
3
replies
299
views
Biological replicates in RNA-seq
RNA-seq biological-replicates
updated 2 days ago by ★ 1.8k • written 3 days ago by • 0
0
votes
0
replies
109
views
BEAST Divergence time Analysis
time BEAST BEAUti divergence
2 days ago by ahmadjoyyia ▴ 10
0
votes
0
replies
125
views
Differential Gene Expression analysis in heterogeneous tissues
RNA-seq deseq2
updated 2 days ago by 39k • written 2 days ago by ▴ 70
2
votes
2
replies
265
views
Plotting heat map with significance based on multiple columns
R Python Heatmap
updated 2 days ago by 3.7k • written 3 days ago by ▴ 50
1
vote
1
reply
162
views
Issue with MiSeq Demultiplexing: Unexpected Ns in I5_Index
MiSeq Demultiplexing Sequencing
updated 2 days ago by 129k • written 2 days ago by • 0
4
votes
5
replies
460
views
bitr() doesn't recognize the keys I've entered as valid gene symbols for my organism's database ('SYMBOL').
pathway-analysis clusterProfiler
2 days ago by Medeea • 0
3
votes
2
replies
277
views
vg: Mapping fewer reads to a genome graph than a linear reference
giraffe mapping pangenome vg
updated 1 day ago by ▴ 470 • written 3 days ago by ▴ 20
0
votes
1
reply
174
views
Variant Annotation
Variant-Annotation VCF R
updated 2 days ago by 39k • written 2 days ago by • 0
0
votes
0
replies
138
views
News: course - Advanced Python programming
Programming DeepLearning Python MachineLearning
2 days ago by carlopecoraro2 ★ 2.3k
0
votes
1
reply
200
views
too many zeros in 16S rRNA amplicon sequencing data
16S rRNA
updated 23 hours ago by 3.3k • written 2 days ago by ▴ 180
0
votes
0
replies
158
views
Job: Looking for a internship or master's course in Bioinformatics
masters internship career
updated 2 days ago by 39k • written 2 days ago by • 0
0
votes
1
reply
196
views
Seurat 'DoHeatmap()' hierarchical clustering
DoHeatmap Seurat clustering
updated 2 days ago by ▴ 940 • written 2 days ago by • 0
3
votes
2
replies
259
views
Bioinformatics in drug designing
pharmaceutical
2 days ago by nishimalhotra2612 ▴ 40
0
votes
1
reply
161
views
Finding Kinetic Parameters from Western Blot images
image-processing western-blot
updated 2 days ago by 39k • written 2 days ago by • 0
3
votes
1
reply
3.2k
views
How To Interpret The Numbers In The Cnvnator Genotype Output
genotyping
updated 2 days ago by • 0 • written 10.4 years ago by ▴ 150
0
votes
0
replies
137
views
Chipseq data peak calling issue
Bioinformatics sequencing chipseq RNA data
2 days ago by Adyasha • 0
0
votes
2
replies
261
views
NanoSim Error "Please specify the training reads and its reference genome!"
NanoSim RNA-seq LongRead
1 day ago by akainth • 0
3
votes
4
replies
312
views
rose identifies super enhancers, how to output PDF files
PDF rose
updated 2 days ago by 39k • written 3 days ago by • 0
0
votes
0
replies
141
views
Assigned taxonomy after dada2 only "NAs" or "Mitochondria"
16S dada2 DECIPHER ITS taxonomy
2 days ago by mattze731 • 0
0
votes
0
replies
133
views
I want to set the null hypothesis for the test of genetic correlation to rg=1.
correlation LDSC genetic
2 days ago by Apprentice ▴ 130
1,000 results • Page 1 of 20
Recent Votes
Comment: DEgs RNAseq
Answer: ScRNA data
Answer: extract all fasta sequence from db v5
Does the RNAseq data normal if the TPM value 3rd Qutile expression is near 10, but the Max expression are near 20,000
Comment: Does the RNAseq data normal if the TPM value 3rd Qutile expression is near 10, b
Answer: Does the RNAseq data normal if the TPM value 3rd Qutile expression is near 10, b
A: extract dendrogram cluster from pheatmap
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Recent Replies
Comment: Single cell chemistry by David • 0
Unlikely, this data came from a experienced sequencing team, I 'm the inexperienced data analyst. I'm not sure if these samples are 10x, …
Comment: Single cell chemistry by David • 0
Sorry, I did not explain it well. I did it before with other group of samples and different chemistry, so cellranger is working fine in my…
Comment: Do bioinformaticians often break molecular biologists' hearts by being the first by jli.ww • 0
Based on my personal experience as a computational biologist, I was not a co-first author even if I prioritized the candidates from the pub…
Comment: DEgs RNAseq by Juan • 0
Dear Delaney Sullivan, thanks, finlly I did my analisys
Answer: ScRNA data by Dr William Klubinski ▴ 20
It seems like your single cell RNAseq data does not already contain the Cell Barcodes or Unique Molecular Identifiers in the standard way -…
Comment: Demultiplexing bam file by GenoMax 129k
> Part of the header of the bam file So perhaps there are more samples. https://www.biostars.org/u/21477/: `grep "SM*" header_of_bam`. Is…
Answer: extract all fasta sequence from db v5 by GenoMax 129k
If you need the fasta sequence download from NCBI here (146 GB zip compressed file) : https://ftp.ncbi.nih.gov/blast/db/FASTA/nr.gz
Comment: ScRNA data by GenoMax 129k
Is this from a SRA dataset? If so it is possible that you can get the correct information in `DataAccess` tab. Provide the accession number…
Comment: extract all fasta sequence from db v5 by Pierre Lindenbaum 154k
is version 4 compatible with blast v5 ? try to use version 4....
Comment: Pre-processing for Agilent microarray data? by solarchan7 • 0
thank you!
Comment: Does the RNAseq data normal if the TPM value 3rd Qutile expression is near 10, b by alwayshope ▴ 20
Thanks a lot! Very helpful detailed guidance! Appreciate it a lot!
Answer: Does the RNAseq data normal if the TPM value 3rd Qutile expression is near 10, b by Dr William Klubinski ▴ 20
The values you are getting, with a third quartile of appx 10 and a max of appx 20,000, seem quite standard for RNA-Seq analysis. The RNA-se…
Comment: Demultiplexing bam file by Pierre Lindenbaum 154k
you don't talk about the size in your question
Comment: Demultiplexing bam file by hasani.iut6 ▴ 60
so why the size of bam file is 40 gig?
Comment: Demultiplexing bam file by Pierre Lindenbaum 154k
> I want to separate every sample I don't understand. In your header there is only one sample : "`13.02.1402_POOL2"`
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