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196 results • Page
3 of 4
Sort: replies
Rank
Views
Votes
Replies
3
votes
3
replies
298
views
How to get ncol = nrow?
DESeq2
updated 1 day ago by
Ram
40k • written 2 days ago by
Suha
• 0
1
vote
3
replies
1.1k
views
GenotypeGVCF too many genotypes from pooled samples
GenotypeGVCFs
GATK
VCF
updated 1 day ago by
Paula Andrea
• 0 • written 2.4 years ago by
Vic
▴ 90
1
vote
3
replies
920
views
Genewiz gave me bam files instead of fasta files- can I convert bam to fasta to have a genome assembly?
bam
samtools
fasta
updated 1 day ago by
Ram
40k • written 2.1 years ago by
kristina.mahan
▴ 160
1
vote
3
replies
962
views
How to reduce Quast stats being based on "contigs of size > 100000 bp"
quast
updated 1 day ago by
Ram
40k • written 3.1 years ago by
kristina.mahan
▴ 160
3
votes
3
replies
760
views
How to extract the first and last 2 nt from multiple sequences in a FASTA file?
perl
awk
fasta
genome
sequence
updated 1 day ago by
Ram
40k • written 2.1 years ago by
praasu
▴ 40
0
votes
3
replies
287
views
QC of genetic data
PLINK
updated 6 hours ago by
bk11
★ 1.4k • written 4 days ago by
kl
▴ 10
6
votes
3
replies
6.8k
views
Identifying discordantly mapped reads
bam
samtools
paired-end
updated 6 hours ago by
Ram
40k • written 7.9 years ago by
Linda
▴ 150
2
votes
3
replies
109
views
Forum:
How do you validate and verify your pipeline's software updates?
Validation
Verification
Quality-Assurance
updated 3 hours ago by
ATpoint
77k • written 4 hours ago by
kieran
• 0
1
vote
2
replies
300
views
OMA in AWS cloud
OMA
AWS
updated 6 days ago by
Adrian Altenhoff
★ 1.1k • written 12 days ago by
Ksel
• 0
0
votes
2
replies
246
views
Quality Control of VCFs that used different genotyping arrays
bcftools
VCF
1 day ago by
Shane
• 0
0
votes
2
replies
1.1k
views
Blobtools output files
blobtools
updated 1 day ago by
Ram
40k • written 3.0 years ago by
kristina.mahan
▴ 160
1
vote
2
replies
3.2k
views
rna-seq analysis with Salmon - how to Import and summarize using tximport
RNA-Seq
salmon
tximport
updated 1 day ago by
camillab.
▴ 140 • written 4.1 years ago by
woojoy14
▴ 10
0
votes
2
replies
115
views
Get discordant read pairs from bam
samtools
NGS
updated 7 hours ago by
benformatics
3.8k • written 8 hours ago by
paulaotero.sanchez
• 0
0
votes
2
replies
152
views
I need help with univariate logistic regression in a set of microarray data
R
microarray
odds-ratio
updated 7 hours ago by
Ram
40k • written 21 hours ago by
Pooria
• 0
1
vote
2
replies
185
views
extract first and last n bp from fasta file from multiple fasta file in R
comparative-genomics
updated 1 day ago by
Ram
40k • written 1 day ago by
praasu
▴ 40
1
vote
2
replies
302
views
functional analysis
16S
metabarcoding
functionalanalysis
1 day ago by
safeassli
• 0
0
votes
2
replies
234
views
ptt and rnt files that were created from assembly rather than a genome
rockhopper
genbank
5 days ago by
langziv
▴ 50
0
votes
2
replies
269
views
Issues with featureCounts
featurecounts
rna-seq
differential-expression
updated 11 hours ago by
biofalconch
★ 1.0k • written 4 days ago by
Aime
• 0
0
votes
2
replies
179
views
GOSt Plot
GOSt
ggplot2
updated 1 day ago by
bk11
★ 1.4k • written 1 day ago by
Sarah
• 0
0
votes
2
replies
178
views
chromosome location
visualization
updated 6 days ago by
Ram
40k • written 6 days ago by
Farzana
• 0
2
votes
2
replies
824
views
Will this de novo transcriptome assembly be useful in looking at RNAseq differential expression?
de-novo-assembly
updated 1 day ago by
Ram
40k • written 2.2 years ago by
kristina.mahan
▴ 160
3
votes
2
replies
89
views
Feature plot not applicable for RunTSNE object in seurat?
harmony
Seurat
single-cell
5 hours ago by
synat.keam
▴ 80
0
votes
2
replies
196
views
Detect STRs in illumina library
repeats
annotation
STR
5 days ago by
kirillkirilenko
▴ 20
0
votes
2
replies
226
views
Splitting VCF/BCF file into seperate gene files
genomics
bcftools
eqtl
gwas
updated 5 days ago by
Pierre Lindenbaum
157k • written 5 days ago by
Lynne-95
• 0
0
votes
2
replies
263
views
MAKER: WARNING: Could not get initialization lock. Trying Again...
gene-prediction
MAKER
updated 1 day ago by
Ram
40k • written 3 days ago by
memy
▴ 20
0
votes
2
replies
1.4k
views
Converting hdf5 file to loom file
single-cell
python
scvelo
RNA
updated 5 days ago by
bk11
★ 1.4k • written 2.4 years ago by
sidrah.maryam
▴ 50
2
votes
2
replies
251
views
Get support value of MRCA given a list of tip.label in R newick trees
phylogeny
newick
R
ape
tree
5 days ago by
pl.terzian
• 0
0
votes
2
replies
166
views
How can I compute energy from a protein PDB file?
pdb
protein
updated 13 hours ago by
Matthias Zepper
4.1k • written 1 day ago by
4fzcgueyp5
• 0
2
votes
2
replies
1.0k
views
How to check for completeness of a mitochondrial or chloroplast genome?
genome-completeness
organelles
updated 1 day ago by
Ram
40k • written 2.6 years ago by
kristina.mahan
▴ 160
0
votes
2
replies
882
views
RSEM not giving .genes.results and isoforms.results ; Plase check if you provide correct parameters/options for the pipeline!
genomics
transcriptome
rsem
ngs
5 days ago by
kat.bi
• 0
1
vote
2
replies
396
views
The bam file used for DiffBind?
DiffBind
ATAC-seq
5 days ago by
Miao Zhang
• 0
2
votes
2
replies
292
views
bedtools intersect by position & stand not working even with common regions
bedtools
genomic
intersect
bedops
intervals
1 day ago by
Alewa
▴ 140
0
votes
2
replies
149
views
Generating a 3D model from 2D slices of a nucleus
imaging
modeling
5 hours ago by
jachimowicz9002
▴ 10
2
votes
2
replies
1.9k
views
Plotting Allele Frequencies
variant-analysis
updated 1 day ago by
Ram
40k • written 4.2 years ago by
kristina.mahan
▴ 160
1
vote
1
reply
184
views
Transcription factor co-localization p-values
p-values
updated 6 days ago by
jared.andrews07
★ 15k • written 6 days ago by
Vishal
▴ 10
0
votes
1
reply
37
views
Kimura% of calcDivergenceFromAlign output
repeatlandscape
repeatmasker
2 hours ago by
睿紘
• 0
1
vote
1
reply
196
views
vcf.gz to vcf
VCF
updated 2 days ago by
Mark
★ 1.3k • written 2 days ago by
sooni
▴ 10
0
votes
1
reply
1.3k
views
Convert .sqn file to .gbk
organelle
updated 1 day ago by
Ram
40k • written 2.6 years ago by
kristina.mahan
▴ 160
0
votes
1
reply
198
views
How to import bigwig files into igvR
bigwig
igvr
updated 3 days ago by
Trivas
★ 1.5k • written 3 days ago by
ntsopoul
▴ 50
0
votes
1
reply
143
views
Calculating haplotype and nucleotide diversity with deep amplicon sequencing data
haplotype
diversity
Pegas
Arlequin
HTS_data
6 days ago by
deorugz
• 0
0
votes
1
reply
873
views
How to use gff file of old annotation to annotate new assembly
chloroplast
gff
updated 1 day ago by
Ram
40k • written 3.0 years ago by
kristina.mahan
▴ 160
0
votes
1
reply
202
views
Unable to install HorvathMammalMethylChip40manifest packages
r
programming
updated 3 days ago by
ATpoint
77k • written 3 days ago by
Nibedita
• 0
1
vote
1
reply
844
views
How to remove circular contigs from assembly.fasta
de-novo-genome-assembly
ngs
updated 1 day ago by
Ram
40k • written 3.2 years ago by
kristina.mahan
▴ 160
1
vote
1
reply
1.7k
views
How to extract a range of nucleotides from a fasta file
fasta
updated 1 day ago by
Ram
40k • written 3.2 years ago by
kristina.mahan
▴ 160
1
vote
1
reply
214
views
Imputation server failing to see samples in VCF files
imputation
VCF
updated 4 days ago by
Ram
40k • written 4 days ago by
Ben
• 0
1
vote
1
reply
1.8k
views
Using Blobtools to remove sequence contamination
blobtools
updated 1 day ago by
Ram
40k • written 3.3 years ago by
kristina.mahan
▴ 160
0
votes
1
reply
536
views
Recommendations for extending contigs from denovo assembly to identify SV insertion sites on chromosome
Nanopore
WGS
Long-Read
SV
Assembly
4 days ago by
dk0319
▴ 70
2
votes
1
reply
250
views
TPM RNA-seq data for differential expression analysis
RNA-seq
updated 4 days ago by
Ram
40k • written 4 days ago by
Catalin-George
• 0
0
votes
1
reply
192
views
Lower alignment rate when using collapsed reads
short_rna
RNA-Seq_mapping
collapsed_reads
updated 4 days ago by
LChart
3.4k • written 5 days ago by
blz
▴ 30
1
vote
1
reply
226
views
Comparing multiple RNASeq studies
RNASeq
updated 5 days ago by
Zhenyu Zhang
▴ 980 • written 5 days ago by
Luke
▴ 10
196 results • Page
3 of 4
Recent Votes
Answer: tensorQTL interaction issue
Answer: Script for getting summary statistic of any genome using GTF or GFF3 ?
Comment: How do you validate and verify your pipeline's software updates?
Comment: How do you validate and verify your pipeline's software updates?
Answer: GSEA with GO and KEGG datasets
GSEA with GO and KEGG datasets
Answer: Exporting DEGs obtained from DESeq2
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Recent Replies
Answer: tensorQTL interaction issue
by
Solal
• 0
Writing a solution for anyone interested. The issue stems from a dosage error, some variant contains only heterozygous individuals and no h…
Answer: The Biostar Handbook. A bioinformatics e-book for beginners.
by
Qboy
• 0
Great book! I love using it to help my mentees to learn bioinformatics better. It would be also fantastic to compile all other necessary Pr…
Comment: Rockhoppper - Suspiciously low percentage of perfectly aligned reads
by
langziv
▴ 50
Thanks. I'll try.
Comment: How to get sample names and genotype for SNP in multi-sample VCF file
by
avelarbio46
▴ 30
Hi! I'm trying to parallelize your code (to run on a bunch of files), but it is not working because of multiple processes spawning. Do you …
Comment: Rockhoppper - Suspiciously low percentage of perfectly aligned reads
by
GenoMax
135k
> so I don't think the genome size is available. Perhaps not for your specific strain but plenty of other Kpn genomes are available and th…
Comment: Kimura% of calcDivergenceFromAlign output
by
睿紘
• 0
For additional information, "Coverage for each repeat class and divergence (Kimura)" is also the result from calcDivergenceFromAlign.pl
Comment: Rockhoppper - Suspiciously low percentage of perfectly aligned reads
by
langziv
▴ 50
By the way, I run Rockhopper on the untrimmed files, and the full match percentage grew to 20%-30%, and there are 1552 transcripts in the u…
Comment: Rockhoppper - Suspiciously low percentage of perfectly aligned reads
by
langziv
▴ 50
Here are the total bases in 4 fastq files (after adapter removal): 1,027,852,411, 1,041,895,083, 1,121,786,520, 1,299,721,929. I just rem…
Comment: Understanding mother and father alleles in VCF file
by
c.
• 0
actually they didn't tell me which relatives were from mother side and which from father side, you are right. But how can you find related …
Comment: Rockhoppper - Suspiciously low percentage of perfectly aligned reads
by
GenoMax
135k
Correct. Data that went into the assembly.
Comment: Rockhoppper - Suspiciously low percentage of perfectly aligned reads
by
langziv
▴ 50
Total bases (number of reads * read length) should be calculated after removal of adapters, right?
Comment: Getting BLAST to give output whilst running
by
Ken
• 0
If I understand correctly, Blast can tell you how many results it has gotten but it can't tell you how many it is going to get, therefore i…
Comment: Understanding mother and father alleles in VCF file
by
Ram
40k
I don't understand your logic. You don't know what phasing is but are confident it is not required, and your proof is that relatives were d…
Comment: Understanding mother and father alleles in VCF file
by
c.
• 0
It's from Nebula genomics. It must be possible to deduce parental alleles, since I converted the VCF file into a Myheritage VCF file and th…
Comment: Deseq2 error
by
sgadila
• 0
Sorry, I just edited my post.
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