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892 results • Page
6 of 18
Sort: Rank
Rank
Views
Votes
Replies
1
vote
7
replies
474
views
Downsampling long-read BAM files
nanopore
BAM
QC
ONT
downsampling
11 days ago by
eebloom
▴ 80
3
votes
3
replies
355
views
What analysis suitable to identify similarly expressed genes between two samples (treated vs untreated). Contrary to DGE analysis,
Gene-Expression
RNA
NOISeq
DGE
updated 11 days ago by
ATpoint
82k • written 12 days ago by
alifafiq1
• 0
0
votes
0
replies
104
views
Use of annotation of integrated Seurat object in single sample
Seurat
12 days ago by
Bine
▴ 60
0
votes
0
replies
112
views
News:
Beyond beginner R: Advancing your coding skills - September 16-19
Advanced-Coding
Programming
R
updated 12 days ago by
Ram
43k • written 12 days ago by
carlopecoraro2
★ 2.5k
0
votes
1
reply
247
views
Subsetting and merging back Seurat Object brings different results
Seurat
12 days ago by
Bine
▴ 60
0
votes
0
replies
169
views
Job:
Postdoctoral Position in Bioinformatics - Mainz, Germany
NGS
Genomics
Post-doc
R-loops
updated 12 days ago by
Ram
43k • written 12 days ago by
4r-rtg
• 0
1
vote
1
reply
152
views
Discrepancy in read counts from fastq
downsampling
fastq
updated 12 days ago by
Ram
43k • written 12 days ago by
marco.barr
▴ 110
0
votes
0
replies
103
views
Successfully identified and Masked rpeats using RM. What Next?
sequence
annotation
repeatmasker
illumina
assembly
12 days ago by
Vijith
▴ 30
10
votes
14
replies
715
views
6 follow
Bacterial plasmid analysis
bacteria
plasmid
wgs
hybridassembly
sequencing
updated 7 days ago by
GenoMax
142k • written 13 days ago by
nicole.kavanagh
• 0
0
votes
1
reply
291
views
Is there a way to increase the automatic label text size in Cytoscape?
Cytoscape
updated 12 days ago by
Scooter
▴ 280 • written 19 days ago by
avocado123
• 0
0
votes
0
replies
107
views
Combining Methylation Microarray data from different platforms
Microarray
DNA
EPIC
450K
Methylation
12 days ago by
James
▴ 10
0
votes
1
reply
600
views
Basic stats in hierfstat
Fst
stats
hierfstat
updated 12 days ago by
mdav
• 0 • written 19 months ago by
Zoe
• 0
1
vote
3
replies
267
views
Odd alignment question/finding
Alignment
updated 11 days ago by
barslmn
★ 2.1k • written 12 days ago by
poordumbsillyidiot
• 0
0
votes
1
reply
222
views
GATK won't produce figures while analyzing covariates to generate 'recal_plots.pdf'
GATK
recalibration
updated 12 days ago by
GenoMax
142k • written 12 days ago by
mgranada3
▴ 30
0
votes
0
replies
132
views
Looking for the best way to interpret these data
RNA-seq
ontology
GO
updated 12 days ago by
Ram
43k • written 12 days ago by
Oscar
▴ 10
0
votes
7
replies
608
views
Post-imputation plot
michigan-imputation-server
quality-control
updated 11 days ago by
LChart
3.9k • written 15 days ago by
kl
▴ 10
0
votes
1
reply
237
views
How to find overlap of clusters in two seurat porject
clustering
seurat
updated 13 days ago by
Adam
▴ 30 • written 14 days ago by
J
• 0
1
vote
3
replies
342
views
DESeq2 processing problems
deseq2
R
updated 13 days ago by
ATpoint
82k • written 13 days ago by
jagdish7921
• 0
0
votes
1
reply
790
views
cnetplot category names are too long
clusterprofiler
RNA-Seq
enrichplot
cnetplot
updated 12 days ago by
Ram
43k • written 2.7 years ago by
carov
• 0
0
votes
1
reply
168
views
Mutect2 for mitochondria variant discovery
mutect2
mitochondria
updated 13 days ago by
Ram
43k • written 13 days ago by
ernestine.kubi
• 0
0
votes
0
replies
114
views
Tools to circularise the ion torrent bacterial genome assembly
iontorrent
bacteria
circularise
13 days ago by
VITALA
• 0
1
vote
2
replies
280
views
POSSUM not working due to incompatible pssm file
fasta
pse-pssm
pssm
POSSUM
12 days ago by
rianna.collins
• 0
0
votes
1
reply
198
views
Alternatives to metanalyisis. P integration. Horiztonal integration RNA-seq
rna-seq
updated 13 days ago by
i.sudbery
19k • written 13 days ago by
Edmond
• 0
0
votes
0
replies
156
views
What open-source tools can determine the coverage/completion of a pathway based on EC numbers?
metagenomics
metacyc
enzyme
pathway
genomics
13 days ago by
O.rka
▴ 720
0
votes
1
reply
697
views
after selecting specific GO terms to plot, cneplot is not showing any color for genes, GO terms and only showing lines without nodes.
cneplot
updated 13 days ago by
Ram
43k • written 13 days ago by
Dr Huma Naz
• 0
2
votes
2
replies
299
views
p-value in CIBERSORT
CIBERSORT
p-value
13 days ago by
michelafrancesconi9
▴ 20
0
votes
5
replies
411
views
Telescope issue
Telescope
RNA-seq
updated 13 days ago by
GenoMax
142k • written 14 days ago by
eleven11
• 0
5
votes
6
replies
569
views
Programmatically retrieving positions of protein active site residues
Uniprot
PDB
Proteins
updated 14 days ago by
me
▴ 760 • written 15 days ago by
Mariana
▴ 40
1
vote
1
reply
250
views
Adjust pvalue in R with different approach give me all different outcome
statistics
R
p-value
updated 13 days ago by
Ram
43k • written 14 days ago by
Jonathan Yoou
▴ 60
1
vote
2
replies
314
views
Minimum RAM and Storage requirement for creating PSSM using ncbi-blast-2.2.30+-x64-linux
blast
pssm
13 days ago by
Nafi
• 0
1
vote
3
replies
314
views
What does 'bundle_uuid' refer to in this metadata sheet?
SRA
illumina
GEO
metadata
updated 13 days ago by
GenoMax
142k • written 14 days ago by
jeffrey.maurer.informatics
• 0
1
vote
3
replies
310
views
ERROR When Using Ensembl Plants Biomart Wget API
biomart
plant
ensembl
8 days ago by
Dora
▴ 10
0
votes
0
replies
178
views
News:
Goodbye, Slow Code? How BioNumpy can Redefine Python’s Role in Bioinformatics!
BioNumPy
Python
NumPy
updated 14 days ago by
Ram
43k • written 14 days ago by
Chakri
• 0
0
votes
2
replies
245
views
RNA editing sites based on DNA seq and RNA seq
RNA-editing
7 days ago by
Ahiad Chen Zion
• 0
1
vote
5
replies
361
views
Introduce SNPs on FASTA
simulation
snps
haplotypes
pharmacogenetics
11 days ago by
Riccardo
▴ 10
0
votes
0
replies
139
views
News:
Online course: Meta-analysis in R - last 2 seats left
Meta-Analysis
Statistics
R
updated 14 days ago by
Ram
43k • written 14 days ago by
carlopecoraro2
★ 2.5k
0
votes
0
replies
135
views
Somatic variant calling with Mutect2 using 2 matched-normals for one sample
cancer
mutect
variant-calling
GATK
somatic-variants
updated 14 days ago by
Ram
43k • written 14 days ago by
Alexandros
• 0
0
votes
1
reply
182
views
unable to get feature count results
featureCounts
updated 14 days ago by
Ram
43k • written 14 days ago by
Ravita
• 0
0
votes
3
replies
309
views
KEGG Pathways
r
RNA-seq
updated 14 days ago by
Ram
43k • written 14 days ago by
Sudip
• 0
4
votes
6
replies
3.9k
views
Taking the difference of two VCFs (or removing singletons)
genome
sequencing
singleton
SNP
filter
updated 14 days ago by
Andres
▴ 20 • written 9.6 years ago by
hermathena
▴ 40
0
votes
0
replies
146
views
Seurat object reductions (PCA, UMAP,..) after subsetting data
Seurat
14 days ago by
Bine
▴ 60
0
votes
1
reply
205
views
Deseq2
Deseq2
updated 14 days ago by
Jack Tierney
▴ 360 • written 14 days ago by
Sudip
• 0
1
vote
1
reply
258
views
Polishing genome assembly
polishing
Nanopore
PacBio
updated 14 days ago by
Michael
54k • written 14 days ago by
Lissa Cruz Saavedra
• 0
5
votes
3
replies
307
views
6 follow
Integrate transcriptomic data and proteomics data.
OMICS
updated 14 days ago by
Lluís R.
★ 1.2k • written 14 days ago by
이민경[학생](대학원 융합의과학과)
• 0
2
votes
2
replies
212
views
MA plot of shrunken fold change
MA-plot
Deseq2
updated 14 days ago by
ATpoint
82k • written 14 days ago by
Sudip
• 0
1
vote
2
replies
236
views
Need help for downloading arabdopsis thaliana reference genome fasta file and gtf file
Arabidopsis-thaliana
gtf
reference-genome
updated 14 days ago by
Ram
43k • written 14 days ago by
Ravita
• 0
3
votes
3
replies
254
views
Gene Specific coverage from WGS data
WGS
SARS-CoV2
updated 21 hours ago by
Ram
43k • written 14 days ago by
Adyasha
• 0
0
votes
0
replies
120
views
human gut metagenome taxonomy profile table and KO profile table from big cohort
metagenome
gut
human
14 days ago by
fanglujing
▴ 60
0
votes
0
replies
141
views
Are some metagenomes too complex for MAGs?
metagenomics
metagenome
MAGs
assembly
14 days ago by
Jacob
• 0
0
votes
3
replies
760
views
Why cd-hit-est not work when sequence identity threshold<0.95?
genome
cluster
sequence
updated 14 days ago by
weidonglu
• 0 • written 21 months ago by
JZX
• 0
892 results • Page
6 of 18
Recent Votes
A: Best de novo assembler for insect genome ?
Comment: sci-RNA-seq
The Biostar Herald for Monday, May 13, 2024
Answer: Finding variants within a subset of a BAM file
Answer: Extracting only 4-fold degenerate sites from gene sequences/alignments?
Answer: Extracting only 4-fold degenerate sites from gene sequences/alignments?
Answer: Help with IGV abbreviation
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Recent Replies
Answer: RNAseq RNA content
by
noodle
▴ 580
There are many tools for this. I recommend [gatk (picard) CollectRnaSeqMetrics][1] or alternatively [RSeQC][2] and then run [multiqc][3…
Comment: Where to get the following bed file?
by
wyuan37
• 0
Hi, I emailed them and did get the bed file! Thank you so much.
Comment: Where to get the following bed file?
by
wyuan37
• 0
Thanks! Super helpful.
Answer: Post-imputation QC for input into GWAS analyses
by
LauferVA
4.2k
1. Imputation algorithms will output log files indicating, among other things, accuracy of imputation. We can't comment on specifics as we …
Comment: Using Cutadapt to trim adapters from paired-end small RNA sequence data
by
GenoMax
142k
You can simply ignore read 2. It is not adding any information since your small RNA's are going to be small and were completely sequenced b…
Comment: Overlapping Ranges within Granges object
by
ntsopoul
▴ 60
does this work with a single .bed file?
Comment: Finding variants within a subset of a BAM file
by
ramiro.barrantes
• 0
Actually, I found a solution in downloading "bam slices" from TCGA ([https://docs.gdc.cancer.gov/API/Users_Guide/BAM_Slicing/][1]) , which …
Comment: Overlapping Ranges within Granges object
by
ntsopoul
▴ 60
![enter image description here][1]Here is some representative data and an image in igv of one gene. I have loaded the .bed file in igv. As …
Answer: RNAseq RNA content
by
ntsopoul
▴ 60
I guess he/she means raw RNA. Roughly 2% of all RNA is mRNA the rest is rRNA. I think the first thing you need to figure out is with which …
Comment: RNAseq RNA content
by
ATpoint
82k
What is "row RNA"?
Comment: Overlapping Ranges within Granges object
by
ATpoint
82k
I would probably collapse overlapping regions with `reduce` to create the second GRanges. Can you add some representative data? Using dput?
Comment: Overlapping Ranges within Granges object
by
ntsopoul
▴ 60
I have one Granges object and not two. Every IRanges entry is the critical region of the gRNA. Can this still work?
Answer: Extracting only 4-fold degenerate sites from gene sequences/alignments?
by
J.
▴ 40
FYI if you also have this problem, this seems to be a pretty good solution: https://github.com/harvardinformatics/degenotate
Comment: Biomart issue, why so few 3'utrs?
by
GenoMax
142k
AFAIK "MANE" project is only for human protein-coding genes. One potential explanation. Out of the 2000 ID's only 125 may be MANE. https:…
Answer: Biomart issue, why so few 3'utrs?
by
i.sudbery
19k
The MANE-select transcripts are pairs of identically annotated transcripts in ReqSeq and Ensembl. The 3' UTR is the region where otherwise …
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