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Recent Replies
Comment: Raw counts using stringtie
by
ahmad.sajad4541
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![stringtie error][1]
[1]: /media/images/6475a009-e1a6-412b-8515-a8aec173
Answer: Differential Expression Analysis using DESeq2 in R
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![The below image contains the code and result also.][1]
[1]: /media/images/96562b96-d508-4f67-96d1-7c71aa17
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My response above was actually partly inspired off of a reading of your paper :)
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> everything is showing equal in environment section. But when I am checking for equalities, it’s showing False
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You can calculate the percentage of intronic reads for each cell using the [DropletQC][1] package for R.
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I did say almost - obviously things are going to get tricky at gene ends - this is why we have the concept of effective length. Its interst…
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Malat1 correlates with the intronic content and can be used as a nuclear indicator. In this preprint we discuss about this artifact and the…
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I wouldn't say it's true that number of reads is linearly proportional to the length of the gene. That's just an assumption that people mak…
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Have you tried
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I didn't use the same tool as in the paper...
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Yes good idea. I guess the quality of the SV calls and as a proxy for quality the length and distribution of variants called might be infor…
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I just realised I didn't align my reads with the tools they used and used bowtie2 instead. I can't download the older version that is menti…
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