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Is this what you used ultimately? Did this tool satisfy the "specific average depth" requirement?
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Thank you. My budget is around 20 thousand dollars.
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Prefetch files using sratoolkit **prefetch** command and then use **fasterq-dump**. There you can see the number of reads. For validation, …
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random downsampling of bam files is now possible using [rasusa][1] [v1.0.0][2] with the `aln` subcommand
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Use `vdb-validate` included in `sratoolkit` to check your `*.sra` files for integrity.
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