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I have one Granges object and not two. Every IRanges entry is the critical region of the gRNA. Can this still work?
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FYI if you also have this problem, this seems to be a pretty good solution: https://github.com/harvardinformatics/degenotate
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Not found in https://ftp.sanger.ac.uk/resources/software/gwava/
![][1]
[1]: /media/images/f69695ec-e047-44f6-95ce-cd4edf1c
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https://www.sanger.ac.uk/tool/gwava/
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